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Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran.

Maghsoodloorad S, Haghighi A, Sharifi Sarasiabi K, Taghipoor N, Hosseinzadeh N, Gachkar L, Nazemalhosseini Mojarrad E, Maghsoodloorad E - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene.However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

ABSTRACT

Background: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine.

Method: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing.

Results: Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.

Conclusion: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

No MeSH data available.


Schematic representation and nucleotide sequences of 705 bp product of the semi-nested PCR of the Pvdhps gene. The primer’s VDHPS-NF and VDHPS-NR annealing sites were underlined. Bold and underlined letters indicate Codon 421 where mutation occurred
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Figure 2: Schematic representation and nucleotide sequences of 705 bp product of the semi-nested PCR of the Pvdhps gene. The primer’s VDHPS-NF and VDHPS-NR annealing sites were underlined. Bold and underlined letters indicate Codon 421 where mutation occurred

Mentions: PCR products derived from all samples and 705-nucleotide sections were sequenced. No mutations were detected by PCR and sequencing in the samples. However, among 118 samples, three isolates (2.54%) had an as-yet-unreported mutation in the new codon 421 (Table 1). The isolates were on the same position and had only one mutation. A difference in codon 421 was first detected in the present study between the sequence of three isolates obtained from P. vivax and nucleotide sequence of the gens in GenBank (Fig. 2). Sequence of this codon was in the non-mutant isolate GTG where mutant turned into GAG. Because of this mutation, valine is turned into glutamic acid. Sequence of Minab and Jask samples were registered in GenBank bearing no. AB609599 and AB609600, respectively.


Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran.

Maghsoodloorad S, Haghighi A, Sharifi Sarasiabi K, Taghipoor N, Hosseinzadeh N, Gachkar L, Nazemalhosseini Mojarrad E, Maghsoodloorad E - Iran J Parasitol (2016 Jan-Mar)

Schematic representation and nucleotide sequences of 705 bp product of the semi-nested PCR of the Pvdhps gene. The primer’s VDHPS-NF and VDHPS-NR annealing sites were underlined. Bold and underlined letters indicate Codon 421 where mutation occurred
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835476&req=5

Figure 2: Schematic representation and nucleotide sequences of 705 bp product of the semi-nested PCR of the Pvdhps gene. The primer’s VDHPS-NF and VDHPS-NR annealing sites were underlined. Bold and underlined letters indicate Codon 421 where mutation occurred
Mentions: PCR products derived from all samples and 705-nucleotide sections were sequenced. No mutations were detected by PCR and sequencing in the samples. However, among 118 samples, three isolates (2.54%) had an as-yet-unreported mutation in the new codon 421 (Table 1). The isolates were on the same position and had only one mutation. A difference in codon 421 was first detected in the present study between the sequence of three isolates obtained from P. vivax and nucleotide sequence of the gens in GenBank (Fig. 2). Sequence of this codon was in the non-mutant isolate GTG where mutant turned into GAG. Because of this mutation, valine is turned into glutamic acid. Sequence of Minab and Jask samples were registered in GenBank bearing no. AB609599 and AB609600, respectively.

Bottom Line: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene.However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

ABSTRACT

Background: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine.

Method: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing.

Results: Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.

Conclusion: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

No MeSH data available.