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Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran.

Maghsoodloorad S, Haghighi A, Sharifi Sarasiabi K, Taghipoor N, Hosseinzadeh N, Gachkar L, Nazemalhosseini Mojarrad E, Maghsoodloorad E - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene.However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

ABSTRACT

Background: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine.

Method: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing.

Results: Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.

Conclusion: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

No MeSH data available.


Electrophoresis image of bp705 section of Pvdhps gene with nine isolates of P. vivax in Hormozgan ProvinceM: 100bp DNA Ladder Numbers: The isolated numbers PC: Positive Control NC: Negative Control
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Figure 1: Electrophoresis image of bp705 section of Pvdhps gene with nine isolates of P. vivax in Hormozgan ProvinceM: 100bp DNA Ladder Numbers: The isolated numbers PC: Positive Control NC: Negative Control

Mentions: Of 118 samples infested by P. vivax in Minab and Jask in Hormozgan Province, 46 and 72 samples belonged to Minab and Jask, respectively. Seventy-nine patients (67%) were male. The average age was 22 yr and the subjects were mostly Iranians (91.5%). Nested-PCR products of nine samples were shown in (Fig. 1) with 705 nucleotides. All isolates showed identical bands to the ones in (Fig. 1).


Genetic Diversity of Dihydropteroate synthetase Gene (dhps) of Plasmodium vivax in Hormozgan Province, Iran.

Maghsoodloorad S, Haghighi A, Sharifi Sarasiabi K, Taghipoor N, Hosseinzadeh N, Gachkar L, Nazemalhosseini Mojarrad E, Maghsoodloorad E - Iran J Parasitol (2016 Jan-Mar)

Electrophoresis image of bp705 section of Pvdhps gene with nine isolates of P. vivax in Hormozgan ProvinceM: 100bp DNA Ladder Numbers: The isolated numbers PC: Positive Control NC: Negative Control
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835476&req=5

Figure 1: Electrophoresis image of bp705 section of Pvdhps gene with nine isolates of P. vivax in Hormozgan ProvinceM: 100bp DNA Ladder Numbers: The isolated numbers PC: Positive Control NC: Negative Control
Mentions: Of 118 samples infested by P. vivax in Minab and Jask in Hormozgan Province, 46 and 72 samples belonged to Minab and Jask, respectively. Seventy-nine patients (67%) were male. The average age was 22 yr and the subjects were mostly Iranians (91.5%). Nested-PCR products of nine samples were shown in (Fig. 1) with 705 nucleotides. All isolates showed identical bands to the ones in (Fig. 1).

Bottom Line: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene.However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

ABSTRACT

Background: The present study was formulated in order to determine polymorphism of dihydropteroate synthetase gene (dhps) of Plasmodium vivax (P. vivax) in Hormozgan Province, southern Iran and mutations at codons 382, 383, 512, 553, and 585 associated with resistance of P. vivax to sulfadoxine.

Method: One-hundred eighteen isolates of P. vivax were prepared within 2007-2008 to determine dihydrofolate reductase-thymidylate synthase (dhfr-ts) gene. The isolates were determined in the study of genetic diversity of dihydropteroate synthetase gene (dhps) of P. vivax. The study was performed via PCR test and nucleotide sequencing.

Results: Of 118 blood samples infected by P. vivax, 46 and 72 samples belonged to Minab and Jask, respectively. No mutation was detected at 5 target codons. However, among these 118 samples, three isolates (2.54%) were found to have a mutation at the new codon 421.

Conclusion: Since mutation was detected in dihydrofolate reductase (Pvdhfr) gene in the same samples but no mutation was found at five main codons of Pvdhps gene, it can be concluded that P. vivax, considering their mutations in Pvdhfr, is still susceptible to sulfadoxine and therefore, to fansidar in Hormozgan Province, Southern Iran.

No MeSH data available.