Limits...
Microscopic and Molecular Detection of Theileria (Babesia) Equi Infection in Equids of Kurdistan Province, Iran.

Habibi G, Esmaeilnia K, Hablolvarid MH, Afshari A, Zamen M, Bozorgi S - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: The results clearly showed the presence of B. (Theileria) equi DNA in 30 of 31 blood samples (96.77%), but the microscopic examination revealed the 3 of 31 positive Babesia like organisms in the red blood cells (9.67%).The obtained results demonstrated the presence of hidden B. (Theileria) equi infection in horses with previous habitance in Kurdistan Province of Iran.Therefore, hidden infection might be considered as a health threatening and limiting factor in animals used in therapeutic antisera research and production centers.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasite Vaccine Research and Production of Razi Vaccine and Serum Research Institute, Karaj, Iran.

ABSTRACT

Background: Equine piroplasmosis (EP) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of EP is due to the persistent carrier state. Carrier animals to Babesia (Theileria) equi (Laveran 1901) and B. caballi (Nuttall, 1910) infestation could be identified by extremely sensitive PCR-based method. The purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from Kurdistan Province, Iran.

Methods: Thirty one horse and mule blood samples were used with history of living in Kurdistan Province of Iran. The blood specimens were utilized for T. equi and B. caballi DNA identification by PCR and Giemsa stained smears for microscopic observation.

Results: The results clearly showed the presence of B. (Theileria) equi DNA in 30 of 31 blood samples (96.77%), but the microscopic examination revealed the 3 of 31 positive Babesia like organisms in the red blood cells (9.67%).

Conclusion: The obtained results demonstrated the presence of hidden B. (Theileria) equi infection in horses with previous habitance in Kurdistan Province of Iran. The carrier animals became a main source of infection and can transmit the disease. Therefore, hidden infection might be considered as a health threatening and limiting factor in animals used in therapeutic antisera research and production centers.

No MeSH data available.


Related in: MedlinePlus

Gel agarose electrophoresis of PCR targeting Theileria equi 18S rRNA. 1.5% agorose gel showing 18S rRNA amplicons from 8 T. equi infected blood samples. Negative and positive controls as well as molecular size markers are indicated on the right
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4835474&req=5

Figure 2: Gel agarose electrophoresis of PCR targeting Theileria equi 18S rRNA. 1.5% agorose gel showing 18S rRNA amplicons from 8 T. equi infected blood samples. Negative and positive controls as well as molecular size markers are indicated on the right

Mentions: The PCR test showed the isolated DNAs were successfully amplified specifically by using Equine piroplasmosis primers. The outcomes of amplification demonstrated 30 positive out of 31 samples for B. (Theileria) equi (96.77%) but no amplification for B. caballi (Fig. 2).


Microscopic and Molecular Detection of Theileria (Babesia) Equi Infection in Equids of Kurdistan Province, Iran.

Habibi G, Esmaeilnia K, Hablolvarid MH, Afshari A, Zamen M, Bozorgi S - Iran J Parasitol (2016 Jan-Mar)

Gel agarose electrophoresis of PCR targeting Theileria equi 18S rRNA. 1.5% agorose gel showing 18S rRNA amplicons from 8 T. equi infected blood samples. Negative and positive controls as well as molecular size markers are indicated on the right
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835474&req=5

Figure 2: Gel agarose electrophoresis of PCR targeting Theileria equi 18S rRNA. 1.5% agorose gel showing 18S rRNA amplicons from 8 T. equi infected blood samples. Negative and positive controls as well as molecular size markers are indicated on the right
Mentions: The PCR test showed the isolated DNAs were successfully amplified specifically by using Equine piroplasmosis primers. The outcomes of amplification demonstrated 30 positive out of 31 samples for B. (Theileria) equi (96.77%) but no amplification for B. caballi (Fig. 2).

Bottom Line: The results clearly showed the presence of B. (Theileria) equi DNA in 30 of 31 blood samples (96.77%), but the microscopic examination revealed the 3 of 31 positive Babesia like organisms in the red blood cells (9.67%).The obtained results demonstrated the presence of hidden B. (Theileria) equi infection in horses with previous habitance in Kurdistan Province of Iran.Therefore, hidden infection might be considered as a health threatening and limiting factor in animals used in therapeutic antisera research and production centers.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasite Vaccine Research and Production of Razi Vaccine and Serum Research Institute, Karaj, Iran.

ABSTRACT

Background: Equine piroplasmosis (EP) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of EP is due to the persistent carrier state. Carrier animals to Babesia (Theileria) equi (Laveran 1901) and B. caballi (Nuttall, 1910) infestation could be identified by extremely sensitive PCR-based method. The purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from Kurdistan Province, Iran.

Methods: Thirty one horse and mule blood samples were used with history of living in Kurdistan Province of Iran. The blood specimens were utilized for T. equi and B. caballi DNA identification by PCR and Giemsa stained smears for microscopic observation.

Results: The results clearly showed the presence of B. (Theileria) equi DNA in 30 of 31 blood samples (96.77%), but the microscopic examination revealed the 3 of 31 positive Babesia like organisms in the red blood cells (9.67%).

Conclusion: The obtained results demonstrated the presence of hidden B. (Theileria) equi infection in horses with previous habitance in Kurdistan Province of Iran. The carrier animals became a main source of infection and can transmit the disease. Therefore, hidden infection might be considered as a health threatening and limiting factor in animals used in therapeutic antisera research and production centers.

No MeSH data available.


Related in: MedlinePlus