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Identification of Leishmania Species Isolated from Human Cutaneous Leishmaniasis in Mehran, Western Iran Using Nested PCR.

Feiz Haddad MH, Ghasemi E, Maraghi S, Tavala M - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: About 56.3% of patients had a single lesion and a significant correlation was observed between age and number of lesions (P > 0.05).The nested PCR technique was shown to be an effective method with high sensitivity and specificity for identification of human Leishmania parasites.Molecular analysis revealed that parasites isolated from Mehran were identified as L. major and the disease was rural in form.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Dept. of Parasitology, Faculty of Medical Sciences, Ahvaz University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background: The incidence of cutaneous leishmaniasis in the city of Mehran has risen sharply in recent years because the city borders Iraq, which has allowed entrance of different Leishmania strains. These strains have different shapes, periods of disease, and healing of lesions. The present study identified and determined cutaneous leishmaniasis species in this region.

Methods: This cross-sectional study was carried out by preparing slides from 92 patients with suspected cutaneous leishmaniasis lesions from Mehran during 2012-2013. Parasite genomic DNA was extracted and CSB2XF and CSB1XR primers were used to amplify the Leishmania minicircle kDNA regions. The parasite species were detected by specific 13Z and LIR primers by applying nested PCR technique.

Results: All banding patterns were diagnosed as L. major parasite by comparison of standard models with amplified fragments 560 bp in length from bands. The patients were 56.5% male and 43.5% female. The most frequently-infected age group was the 21-30 years group at a rate of 27.2%. About 56.3% of patients had a single lesion and a significant correlation was observed between age and number of lesions (P > 0.05).

Conclusion: The nested PCR technique was shown to be an effective method with high sensitivity and specificity for identification of human Leishmania parasites. Molecular analysis revealed that parasites isolated from Mehran were identified as L. major and the disease was rural in form.

No MeSH data available.


Related in: MedlinePlus

Map of Ilam and its counties in 2014 from Iran Meteorological Organization (13).
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Figure 3: Map of Ilam and its counties in 2014 from Iran Meteorological Organization (13).

Mentions: The results showed that the age group most affected by disease was the 21–30 yr. This concurs with the results from Kasiri et al. for Ilam Province (13) and Ahmadi et al. for Borojerd (24) who both found the highest incidence in the 20–29 yr age group. By contrast, Maraghi et al. reported that in Shush, most cases appeared in patients of less than 10 years of age (25). Although the age pattern for cutaneous leishmaniasis varied, rural disease endemicity, parasite species, and host genome patterns increased in incidence in the younger age groups, which indicates high endemicity for this part of the country (26). Evidence suggests that the epidemiological focus in Mehran can be categorized as hyper endemic (Fig. 3).


Identification of Leishmania Species Isolated from Human Cutaneous Leishmaniasis in Mehran, Western Iran Using Nested PCR.

Feiz Haddad MH, Ghasemi E, Maraghi S, Tavala M - Iran J Parasitol (2016 Jan-Mar)

Map of Ilam and its counties in 2014 from Iran Meteorological Organization (13).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835471&req=5

Figure 3: Map of Ilam and its counties in 2014 from Iran Meteorological Organization (13).
Mentions: The results showed that the age group most affected by disease was the 21–30 yr. This concurs with the results from Kasiri et al. for Ilam Province (13) and Ahmadi et al. for Borojerd (24) who both found the highest incidence in the 20–29 yr age group. By contrast, Maraghi et al. reported that in Shush, most cases appeared in patients of less than 10 years of age (25). Although the age pattern for cutaneous leishmaniasis varied, rural disease endemicity, parasite species, and host genome patterns increased in incidence in the younger age groups, which indicates high endemicity for this part of the country (26). Evidence suggests that the epidemiological focus in Mehran can be categorized as hyper endemic (Fig. 3).

Bottom Line: About 56.3% of patients had a single lesion and a significant correlation was observed between age and number of lesions (P > 0.05).The nested PCR technique was shown to be an effective method with high sensitivity and specificity for identification of human Leishmania parasites.Molecular analysis revealed that parasites isolated from Mehran were identified as L. major and the disease was rural in form.

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Dept. of Parasitology, Faculty of Medical Sciences, Ahvaz University of Medical Sciences, Ahvaz, Iran.

ABSTRACT

Background: The incidence of cutaneous leishmaniasis in the city of Mehran has risen sharply in recent years because the city borders Iraq, which has allowed entrance of different Leishmania strains. These strains have different shapes, periods of disease, and healing of lesions. The present study identified and determined cutaneous leishmaniasis species in this region.

Methods: This cross-sectional study was carried out by preparing slides from 92 patients with suspected cutaneous leishmaniasis lesions from Mehran during 2012-2013. Parasite genomic DNA was extracted and CSB2XF and CSB1XR primers were used to amplify the Leishmania minicircle kDNA regions. The parasite species were detected by specific 13Z and LIR primers by applying nested PCR technique.

Results: All banding patterns were diagnosed as L. major parasite by comparison of standard models with amplified fragments 560 bp in length from bands. The patients were 56.5% male and 43.5% female. The most frequently-infected age group was the 21-30 years group at a rate of 27.2%. About 56.3% of patients had a single lesion and a significant correlation was observed between age and number of lesions (P > 0.05).

Conclusion: The nested PCR technique was shown to be an effective method with high sensitivity and specificity for identification of human Leishmania parasites. Molecular analysis revealed that parasites isolated from Mehran were identified as L. major and the disease was rural in form.

No MeSH data available.


Related in: MedlinePlus