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In Vitro Susceptibility of Iranian Isolates of Trichomonas vaginalis to Metronidazole.

Matini M, Maghsood AH, Mohebali M, Rabiee S, Fallah M, Rezaie S, Rezaeian M - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the trichomonads were determined after 48 h incubation at 35.5 °C.Drug susceptibility assays of the all isolates were carried out two times in triplicate under aerobic and anaerobic conditions.Metronidazole resistance was defined as aerobic MIC ≥50 μg/ml, detected in one isolate.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Metronidazole, a 5-nitroimidazole derivative, is the main antitrichomonal agent of choice for treatment of trichomoniasis. Since 1962, some cases of treatment failure with metronidazole have been reported and recently drug resistance is now on the rise in the world. This study was aimed to determine current susceptibility of Iranian isolates of Trichomonas vaginalis to metronidazole.

Methods: This study was performed on 50 T. vaginalis isolates collected from west and central areas of Iran. After axenisation of the parasites, susceptibility testing was carried out by using serial twofold dilutions of metronidazole (400 to 0.1 μg/ml). The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the trichomonads were determined after 48 h incubation at 35.5 °C. Drug susceptibility assays of the all isolates were carried out two times in triplicate under aerobic and anaerobic conditions.

Results: Ninety-eight percent of the T. vaginalis isolates (49/50) were sensitive to metronidazole. Metronidazole resistance was defined as aerobic MIC ≥50 μg/ml, detected in one isolate. The means of aerobic MICs and MLCs and that of anaerobic MICs of the parasites were 2.91, 1.95 and 0.28 μg/ml, respectively.

Conclusion: This investigation showed in vitro low-level tolerance to metronidazole in a few T. vaginalis isolates that may be leading to the development of drug resistance.

No MeSH data available.


Related in: MedlinePlus

Comparison of metronidazole MICs and MLCs of Iranian T. vaginalis isolates under both aerobic and anaerobic environments
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Related In: Results  -  Collection


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Figure 1: Comparison of metronidazole MICs and MLCs of Iranian T. vaginalis isolates under both aerobic and anaerobic environments

Mentions: Aerobic MIC of metronidazole equal or more than 50 μg/ml considered as metronidazole resistance (12), detected in one isolate. Aerobic MLCs of metronidazole were in the range of 0.1 to 25 μg/ml with a mean of 1.45 μg/ml and a standard deviation of 4.19 μg/ml. Ninety four percent of parasite isolates (47/50) showed aerobic MLCs of metronidazole less than 1.6 μg/ml, two MLCs were 12.8 μg/ml and metronidazole resistant isolate had a MLC equal 25 μg/ml (Fig.1). Aerobic MICs after 24 h were at least one dilution higher than those after 48 h.


In Vitro Susceptibility of Iranian Isolates of Trichomonas vaginalis to Metronidazole.

Matini M, Maghsood AH, Mohebali M, Rabiee S, Fallah M, Rezaie S, Rezaeian M - Iran J Parasitol (2016 Jan-Mar)

Comparison of metronidazole MICs and MLCs of Iranian T. vaginalis isolates under both aerobic and anaerobic environments
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835469&req=5

Figure 1: Comparison of metronidazole MICs and MLCs of Iranian T. vaginalis isolates under both aerobic and anaerobic environments
Mentions: Aerobic MIC of metronidazole equal or more than 50 μg/ml considered as metronidazole resistance (12), detected in one isolate. Aerobic MLCs of metronidazole were in the range of 0.1 to 25 μg/ml with a mean of 1.45 μg/ml and a standard deviation of 4.19 μg/ml. Ninety four percent of parasite isolates (47/50) showed aerobic MLCs of metronidazole less than 1.6 μg/ml, two MLCs were 12.8 μg/ml and metronidazole resistant isolate had a MLC equal 25 μg/ml (Fig.1). Aerobic MICs after 24 h were at least one dilution higher than those after 48 h.

Bottom Line: The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the trichomonads were determined after 48 h incubation at 35.5 °C.Drug susceptibility assays of the all isolates were carried out two times in triplicate under aerobic and anaerobic conditions.Metronidazole resistance was defined as aerobic MIC ≥50 μg/ml, detected in one isolate.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran; Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Metronidazole, a 5-nitroimidazole derivative, is the main antitrichomonal agent of choice for treatment of trichomoniasis. Since 1962, some cases of treatment failure with metronidazole have been reported and recently drug resistance is now on the rise in the world. This study was aimed to determine current susceptibility of Iranian isolates of Trichomonas vaginalis to metronidazole.

Methods: This study was performed on 50 T. vaginalis isolates collected from west and central areas of Iran. After axenisation of the parasites, susceptibility testing was carried out by using serial twofold dilutions of metronidazole (400 to 0.1 μg/ml). The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the trichomonads were determined after 48 h incubation at 35.5 °C. Drug susceptibility assays of the all isolates were carried out two times in triplicate under aerobic and anaerobic conditions.

Results: Ninety-eight percent of the T. vaginalis isolates (49/50) were sensitive to metronidazole. Metronidazole resistance was defined as aerobic MIC ≥50 μg/ml, detected in one isolate. The means of aerobic MICs and MLCs and that of anaerobic MICs of the parasites were 2.91, 1.95 and 0.28 μg/ml, respectively.

Conclusion: This investigation showed in vitro low-level tolerance to metronidazole in a few T. vaginalis isolates that may be leading to the development of drug resistance.

No MeSH data available.


Related in: MedlinePlus