Limits...
Inhibition Effect of pH on the Hatchability of Fasciola Miracidia under Laboratory Conditions.

Yakhchali M, Bahramnejad K - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: The present study was aimed to assess the effects of different pH values on hatching rate of Fasciola miracidia.There were significant differences between the hatching rate of the treatments and that of the control group.Water pH is proven to be a crucial factor affecting the life cycle of Fasciola and its epidemiology.

View Article: PubMed Central - PubMed

Affiliation: Dep. of Pathobiology, Parasitology division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

ABSTRACT

Background: Fasciolosis, caused by the liver flukes of the genus Fasciola, is one of the most prevalent diseases of domestic livestock and human throughout the world, imposing considerable economic losses. The present study was aimed to assess the effects of different pH values on hatching rate of Fasciola miracidia.

Methods: The flukes were isolated from the infected livers of the slaughtered ruminants at the abattoir of Urmia City, Iran, crushed thoroughly and sieved for isolation of the Fasciola eggs. The eggs were washed up several times by PBS (0.01N, pH 7.2). They were incubated at different pH values of 7±0.1 (control) and 3-9.5 (treatments) at 28°C for 16 days.

Results: The maximum hatching rate was observed at pH 7 (14.93±0.65%), while no miracidia were hatched at pH 3 and/or pH 9-9.5. There were significant differences between the hatching rate of the treatments and that of the control group.

Conclusion: Water pH is proven to be a crucial factor affecting the life cycle of Fasciola and its epidemiology.

No MeSH data available.


Related in: MedlinePlus

The growing stages of miracidia in Fasciola eggs (original): first day (a); sixth day (b); ninth day (c); twelfth day (d); sixteenth day (e) (magnification: 400×)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4835467&req=5

Figure 1: The growing stages of miracidia in Fasciola eggs (original): first day (a); sixth day (b); ninth day (c); twelfth day (d); sixteenth day (e) (magnification: 400×)

Mentions: The process of the miracidia formation in the control group was prolonged for 16 d (Fig. 1). On the first day of the study, it was clear and a tangible cellular growth. The cellular mass division was not observed, but the cells began to amass from the second day. Developed embryonic cells were observed as large spots alongside of egg on the 6th day. They became uniformed and seen as large brown spots on the 9th day. On the 12th day, the spots amassed in a corner of the egg. The embryonic tissue differentiation was completed and the miracidia stage of Fasciola was observed inside the egg. The miracidia were emerged through light stress for 20 min.


Inhibition Effect of pH on the Hatchability of Fasciola Miracidia under Laboratory Conditions.

Yakhchali M, Bahramnejad K - Iran J Parasitol (2016 Jan-Mar)

The growing stages of miracidia in Fasciola eggs (original): first day (a); sixth day (b); ninth day (c); twelfth day (d); sixteenth day (e) (magnification: 400×)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835467&req=5

Figure 1: The growing stages of miracidia in Fasciola eggs (original): first day (a); sixth day (b); ninth day (c); twelfth day (d); sixteenth day (e) (magnification: 400×)
Mentions: The process of the miracidia formation in the control group was prolonged for 16 d (Fig. 1). On the first day of the study, it was clear and a tangible cellular growth. The cellular mass division was not observed, but the cells began to amass from the second day. Developed embryonic cells were observed as large spots alongside of egg on the 6th day. They became uniformed and seen as large brown spots on the 9th day. On the 12th day, the spots amassed in a corner of the egg. The embryonic tissue differentiation was completed and the miracidia stage of Fasciola was observed inside the egg. The miracidia were emerged through light stress for 20 min.

Bottom Line: The present study was aimed to assess the effects of different pH values on hatching rate of Fasciola miracidia.There were significant differences between the hatching rate of the treatments and that of the control group.Water pH is proven to be a crucial factor affecting the life cycle of Fasciola and its epidemiology.

View Article: PubMed Central - PubMed

Affiliation: Dep. of Pathobiology, Parasitology division, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

ABSTRACT

Background: Fasciolosis, caused by the liver flukes of the genus Fasciola, is one of the most prevalent diseases of domestic livestock and human throughout the world, imposing considerable economic losses. The present study was aimed to assess the effects of different pH values on hatching rate of Fasciola miracidia.

Methods: The flukes were isolated from the infected livers of the slaughtered ruminants at the abattoir of Urmia City, Iran, crushed thoroughly and sieved for isolation of the Fasciola eggs. The eggs were washed up several times by PBS (0.01N, pH 7.2). They were incubated at different pH values of 7±0.1 (control) and 3-9.5 (treatments) at 28°C for 16 days.

Results: The maximum hatching rate was observed at pH 7 (14.93±0.65%), while no miracidia were hatched at pH 3 and/or pH 9-9.5. There were significant differences between the hatching rate of the treatments and that of the control group.

Conclusion: Water pH is proven to be a crucial factor affecting the life cycle of Fasciola and its epidemiology.

No MeSH data available.


Related in: MedlinePlus