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Molecular Evaluation of a Case of Visceral Leishmaniasis Due to Leishmania tropica in Southwestern Iran.

Sarkari B, Bavarsad Ahmadpour N, Moshfe A, Hajjaran H - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: The patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms.Sequence analysis of the PCR product demonstrated that the case has 99% identity with those of available sequences of L. tropica.Intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of L. major and L. infantum was significantly higher.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
We describe a case of visceral leishmaniasis (VL) due to Leishmania tropica in a 50-year-old Iranian man lived in a VL-endemic area in southwest of Iran. The patient presented with a 3-month history of fever and splenomegaly. Clinical signs and serological findings were suggestive of VL. Spleen biopsy was taken from the patient and intracellular forms of Leishmania amastigotes was seen in Giemsa stained smears. The patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms. DNA was extracted from the microscopic slides of the spleen biopsy and the nagt (N-Acetylglucosamine-1-Phosphate Transferase) gene of Leishmania was PCR-amplified. Sequence analysis of the PCR product demonstrated that the case has 99% identity with those of available sequences of L. tropica. Intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of L. major and L. infantum was significantly higher.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic relationship of nagt sequence of L. tropica isolated from VL patient and L. trpoica, L. major and L. infantum from Iran, using Maximum Likelihood method
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Related In: Results  -  Collection


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Figure 1: Phylogenetic relationship of nagt sequence of L. tropica isolated from VL patient and L. trpoica, L. major and L. infantum from Iran, using Maximum Likelihood method


Molecular Evaluation of a Case of Visceral Leishmaniasis Due to Leishmania tropica in Southwestern Iran.

Sarkari B, Bavarsad Ahmadpour N, Moshfe A, Hajjaran H - Iran J Parasitol (2016 Jan-Mar)

Phylogenetic relationship of nagt sequence of L. tropica isolated from VL patient and L. trpoica, L. major and L. infantum from Iran, using Maximum Likelihood method
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835463&req=5

Figure 1: Phylogenetic relationship of nagt sequence of L. tropica isolated from VL patient and L. trpoica, L. major and L. infantum from Iran, using Maximum Likelihood method
Bottom Line: The patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms.Sequence analysis of the PCR product demonstrated that the case has 99% identity with those of available sequences of L. tropica.Intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of L. major and L. infantum was significantly higher.

View Article: PubMed Central - PubMed

Affiliation: Dept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

ABSTRACT
We describe a case of visceral leishmaniasis (VL) due to Leishmania tropica in a 50-year-old Iranian man lived in a VL-endemic area in southwest of Iran. The patient presented with a 3-month history of fever and splenomegaly. Clinical signs and serological findings were suggestive of VL. Spleen biopsy was taken from the patient and intracellular forms of Leishmania amastigotes was seen in Giemsa stained smears. The patient was treated with pentavalent antimonial compound with complete resolution of his systemic signs and symptoms. DNA was extracted from the microscopic slides of the spleen biopsy and the nagt (N-Acetylglucosamine-1-Phosphate Transferase) gene of Leishmania was PCR-amplified. Sequence analysis of the PCR product demonstrated that the case has 99% identity with those of available sequences of L. tropica. Intra-species variation within isolate was 0-0.1%; whereas, inter-species differences of the isolate with those of L. major and L. infantum was significantly higher.

No MeSH data available.


Related in: MedlinePlus