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Severe Diarrhea Due To Cystoisospora belli Infection in an HTLV-1 Woman.

Shafiei R, Najjari M, Kargar Kheirabad A, Hatam G - Iran J Parasitol (2016 Jan-Mar)

Bottom Line: Serologic testing was negative for HIV but she showed positivity for HTLV-1 infection.Treatment with TMP/SMX led to improvement of her diarrhea but she died after one year due to malabsorption syndrome.Adequate detection of C. belli diarrhea in immunocompromise patients of HTLV1 in endemic area can be cured by TMP/SMX.

View Article: PubMed Central - PubMed

Affiliation: Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran; Dept. of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Cystoisospora belli, formerly Isospora belli, as an opportunistic infection agent, is seen in immunocompromised patients like HTLV-1. We describe here cystoisosporiasis in an HTLV1 Iranian female in Mashhad, northwestern Iran in 2012 who presented with a debilitating diarrheal illness and great weight loss. C. belli was detected in her stool by modified acid-fast staining and then by molecular detection. Serologic testing was negative for HIV but she showed positivity for HTLV-1 infection. Treatment with TMP/SMX led to improvement of her diarrhea but she died after one year due to malabsorption syndrome. Adequate detection of C. belli diarrhea in immunocompromise patients of HTLV1 in endemic area can be cured by TMP/SMX.

No MeSH data available.


Related in: MedlinePlus

Gel electrophoresis of PCR product from a Cystoisospora belli infected patient. L. 100-bp DNA ladder. Lane 1. Negative control. Lane 2. 450bp PCR product of Cystoisospora belli
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Figure 2: Gel electrophoresis of PCR product from a Cystoisospora belli infected patient. L. 100-bp DNA ladder. Lane 1. Negative control. Lane 2. 450bp PCR product of Cystoisospora belli

Mentions: For DNA sequencing, PCR products (450 bp) were purified from the agarose gel (Fig. 2), using PCR purification kit (Bioneer, Korea), and sequenced from both directions (Applied Biosystems, DNA Analyzers Sequencing, Bioneer, Korea, Sanger method), using the same primers as those used in primary PCR. ITS1, SSU- rRNA, of Cystoisospora sp. was analyzed and deposited in GenBank (Accession numbers: KF686747). Ninety-nine percent similarities (representative GenBank accession HM630353: 99% identity, 99% query coverage, BLAST E value 0.0) were obtained in comparison of these sequences with all available data of C. belli in GenBank.


Severe Diarrhea Due To Cystoisospora belli Infection in an HTLV-1 Woman.

Shafiei R, Najjari M, Kargar Kheirabad A, Hatam G - Iran J Parasitol (2016 Jan-Mar)

Gel electrophoresis of PCR product from a Cystoisospora belli infected patient. L. 100-bp DNA ladder. Lane 1. Negative control. Lane 2. 450bp PCR product of Cystoisospora belli
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835462&req=5

Figure 2: Gel electrophoresis of PCR product from a Cystoisospora belli infected patient. L. 100-bp DNA ladder. Lane 1. Negative control. Lane 2. 450bp PCR product of Cystoisospora belli
Mentions: For DNA sequencing, PCR products (450 bp) were purified from the agarose gel (Fig. 2), using PCR purification kit (Bioneer, Korea), and sequenced from both directions (Applied Biosystems, DNA Analyzers Sequencing, Bioneer, Korea, Sanger method), using the same primers as those used in primary PCR. ITS1, SSU- rRNA, of Cystoisospora sp. was analyzed and deposited in GenBank (Accession numbers: KF686747). Ninety-nine percent similarities (representative GenBank accession HM630353: 99% identity, 99% query coverage, BLAST E value 0.0) were obtained in comparison of these sequences with all available data of C. belli in GenBank.

Bottom Line: Serologic testing was negative for HIV but she showed positivity for HTLV-1 infection.Treatment with TMP/SMX led to improvement of her diarrhea but she died after one year due to malabsorption syndrome.Adequate detection of C. belli diarrhea in immunocompromise patients of HTLV1 in endemic area can be cured by TMP/SMX.

View Article: PubMed Central - PubMed

Affiliation: Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran; Dept. of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

ABSTRACT
Cystoisospora belli, formerly Isospora belli, as an opportunistic infection agent, is seen in immunocompromised patients like HTLV-1. We describe here cystoisosporiasis in an HTLV1 Iranian female in Mashhad, northwestern Iran in 2012 who presented with a debilitating diarrheal illness and great weight loss. C. belli was detected in her stool by modified acid-fast staining and then by molecular detection. Serologic testing was negative for HIV but she showed positivity for HTLV-1 infection. Treatment with TMP/SMX led to improvement of her diarrhea but she died after one year due to malabsorption syndrome. Adequate detection of C. belli diarrhea in immunocompromise patients of HTLV1 in endemic area can be cured by TMP/SMX.

No MeSH data available.


Related in: MedlinePlus