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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.

Liu M, Xu J, Lou H, Fan W, Yang J, Zheng S - Front Plant Sci (2016)

Bottom Line: The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status.We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition.These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural SciencesHangzhou, China.

ABSTRACT
Rice bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

No MeSH data available.


VuMATE1p::GUS expression analysis in transgenic Arabidopsis plants. Transgenic seedlings were exposed to 10 μM Al, 10 μM ABA, or 10 μM SA for 9 h. Activation of the VuMATE1 promoter was observed by GUS staining (blue). At least two independent transgenic lines were used to analyze GUS expression. Bars, 100 μm.
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Figure 7: VuMATE1p::GUS expression analysis in transgenic Arabidopsis plants. Transgenic seedlings were exposed to 10 μM Al, 10 μM ABA, or 10 μM SA for 9 h. Activation of the VuMATE1 promoter was observed by GUS staining (blue). At least two independent transgenic lines were used to analyze GUS expression. Bars, 100 μm.

Mentions: In order to examine whether phytohormones, ABA and SA, are involved in the transcriptional regulation of VuMATE1, we compared GUS activities among different treatments, i.e., ABA (10 μM; pH 5.5), SA (10 μM; pH 5.5), or Al (10 μM; pH 5.5). Previous study showed that apart from Al, low pH (5.0) also affect VuMATE1 expression in GUS transgenic lines (Fan et al., 2015). Therefore, in order to clarify the regulatory role of hormones, we used the pH5.5 as normal growth conditions to eliminate the impact of pH. As shown in Figure 7, under normal growth conditions (pH 5.5), GUS activity was undetectable in the entire root apex region. Both ABA and SA could slightly induce GUS activity restricted to elongation zone. Addition of Al resulted in the strong induction of GUS activity at entire root apex region.


Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.

Liu M, Xu J, Lou H, Fan W, Yang J, Zheng S - Front Plant Sci (2016)

VuMATE1p::GUS expression analysis in transgenic Arabidopsis plants. Transgenic seedlings were exposed to 10 μM Al, 10 μM ABA, or 10 μM SA for 9 h. Activation of the VuMATE1 promoter was observed by GUS staining (blue). At least two independent transgenic lines were used to analyze GUS expression. Bars, 100 μm.
© Copyright Policy
Related In: Results  -  Collection

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Figure 7: VuMATE1p::GUS expression analysis in transgenic Arabidopsis plants. Transgenic seedlings were exposed to 10 μM Al, 10 μM ABA, or 10 μM SA for 9 h. Activation of the VuMATE1 promoter was observed by GUS staining (blue). At least two independent transgenic lines were used to analyze GUS expression. Bars, 100 μm.
Mentions: In order to examine whether phytohormones, ABA and SA, are involved in the transcriptional regulation of VuMATE1, we compared GUS activities among different treatments, i.e., ABA (10 μM; pH 5.5), SA (10 μM; pH 5.5), or Al (10 μM; pH 5.5). Previous study showed that apart from Al, low pH (5.0) also affect VuMATE1 expression in GUS transgenic lines (Fan et al., 2015). Therefore, in order to clarify the regulatory role of hormones, we used the pH5.5 as normal growth conditions to eliminate the impact of pH. As shown in Figure 7, under normal growth conditions (pH 5.5), GUS activity was undetectable in the entire root apex region. Both ABA and SA could slightly induce GUS activity restricted to elongation zone. Addition of Al resulted in the strong induction of GUS activity at entire root apex region.

Bottom Line: The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status.We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition.These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural SciencesHangzhou, China.

ABSTRACT
Rice bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

No MeSH data available.