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Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.

Liu M, Xu J, Lou H, Fan W, Yang J, Zheng S - Front Plant Sci (2016)

Bottom Line: The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status.We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition.These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural SciencesHangzhou, China.

ABSTRACT
Rice bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

No MeSH data available.


Related in: MedlinePlus

Citrate secretion from rice bean roots in response to Fe deficiency and Al stress. One-week-old seedlings were subject to nutrient solution with 20 μM Fe (+Fe) or without (-Fe) for 12 days after treatment, root exudates were collected in 0.5 mM CaCl2 solution (pH 4.5) in the absence (-Al) or presence of 25 μM Al (+Al) for 6 h. Data are expressed as means ± SD (n = 4). Columns with different letters indicate significant difference at P < 0.05.
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Figure 5: Citrate secretion from rice bean roots in response to Fe deficiency and Al stress. One-week-old seedlings were subject to nutrient solution with 20 μM Fe (+Fe) or without (-Fe) for 12 days after treatment, root exudates were collected in 0.5 mM CaCl2 solution (pH 4.5) in the absence (-Al) or presence of 25 μM Al (+Al) for 6 h. Data are expressed as means ± SD (n = 4). Columns with different letters indicate significant difference at P < 0.05.

Mentions: Citrate anion secretion from roots can also complex with Fe in the rhizosphere to improve its mobilization and uptake by roots (Jones et al., 1996). The localization of VuMATE1 in the epidermis cells of the root led us to investigate its possible role in mobilizing Fe in the rhizosphere through enhanced citrate secretion. Therefore, we analyzed citrate secretion from whole roots under either +Fe or -Fe conditions and found that Fe deficiency did not increase citrate secretion. However, Al treatment significantly increased citrate secretion regardless of Fe supply (Figure 5).


Characterization of VuMATE1 Expression in Response to Iron Nutrition and Aluminum Stress Reveals Adaptation of Rice Bean (Vigna umbellata) to Acid Soils through Cis Regulation.

Liu M, Xu J, Lou H, Fan W, Yang J, Zheng S - Front Plant Sci (2016)

Citrate secretion from rice bean roots in response to Fe deficiency and Al stress. One-week-old seedlings were subject to nutrient solution with 20 μM Fe (+Fe) or without (-Fe) for 12 days after treatment, root exudates were collected in 0.5 mM CaCl2 solution (pH 4.5) in the absence (-Al) or presence of 25 μM Al (+Al) for 6 h. Data are expressed as means ± SD (n = 4). Columns with different letters indicate significant difference at P < 0.05.
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Related In: Results  -  Collection

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Figure 5: Citrate secretion from rice bean roots in response to Fe deficiency and Al stress. One-week-old seedlings were subject to nutrient solution with 20 μM Fe (+Fe) or without (-Fe) for 12 days after treatment, root exudates were collected in 0.5 mM CaCl2 solution (pH 4.5) in the absence (-Al) or presence of 25 μM Al (+Al) for 6 h. Data are expressed as means ± SD (n = 4). Columns with different letters indicate significant difference at P < 0.05.
Mentions: Citrate anion secretion from roots can also complex with Fe in the rhizosphere to improve its mobilization and uptake by roots (Jones et al., 1996). The localization of VuMATE1 in the epidermis cells of the root led us to investigate its possible role in mobilizing Fe in the rhizosphere through enhanced citrate secretion. Therefore, we analyzed citrate secretion from whole roots under either +Fe or -Fe conditions and found that Fe deficiency did not increase citrate secretion. However, Al treatment significantly increased citrate secretion regardless of Fe supply (Figure 5).

Bottom Line: The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status.We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition.These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural SciencesHangzhou, China.

ABSTRACT
Rice bean (Vigna umbellata) VuMATE1 appears to be constitutively expressed at vascular system but root apex, and Al stress extends its expression to root apex. Whether VuMATE1 participates in both Al tolerance and Fe nutrition, and how VuMATE1 expression is regulated is of great interest. In this study, the role of VuMATE1 in Fe nutrition was characterized through in planta complementation assays. The transcriptional regulation of VuMATE1 was investigated through promoter analysis and promoter-GUS reporter assays. The results showed that the expression of VuMATE1 was regulated by Al stress but not Fe status. Complementation of frd3-1 with VuMATE1 under VuMATE1 promoter could not restore phenotype, but restored with 35SCaMV promoter. Immunostaining of VuMATE1 revealed abnormal localization of VuMATE1 in vasculature. In planta GUS reporter assay identified Al-responsive cis-acting elements resided between -1228 and -574 bp. Promoter analysis revealed several cis-acting elements, but transcription is not simply regulated by one of these elements. We demonstrated that cis regulation of VuMATE1 expression is involved in Al tolerance mechanism, while not involved in Fe nutrition. These results reveal the evolution of VuMATE1 expression for better adaptation of rice bean to acid soils where Al stress imposed but Fe deficiency pressure released.

No MeSH data available.


Related in: MedlinePlus