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Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus

Heat map illustrating the different metabolite levels in tomato plants under different treatments. Each row represents differentially expressed metabolites while each column represents a treatment. Mean values of two independent experiments are presented here. Metabolites are clustered using average linkage hierarchical clustering. The colors in the heat map represent the intensity of the log2-fold change in metabolite levels. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.
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Figure 7: Heat map illustrating the different metabolite levels in tomato plants under different treatments. Each row represents differentially expressed metabolites while each column represents a treatment. Mean values of two independent experiments are presented here. Metabolites are clustered using average linkage hierarchical clustering. The colors in the heat map represent the intensity of the log2-fold change in metabolite levels. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.

Mentions: Metabolites are considered as signaling molecules as they are associated with physiological processes. To elucidate ISR process that may be involved in PAA-mediated resistance to fusarium wilt disease, we analyzed the whole metabolome of tomato plants inoculated with the pathogen and PAA in either combination. Central metabolites changed in response to PAA + F. oxysporum, PAA, and F. oxysporum alone were normalized to respective control and were expressed in fold change (Figure 4). Here we identified more than 60 metabolites whose levels were seemed to be altered in response to different treatments (Figure 7).


Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Heat map illustrating the different metabolite levels in tomato plants under different treatments. Each row represents differentially expressed metabolites while each column represents a treatment. Mean values of two independent experiments are presented here. Metabolites are clustered using average linkage hierarchical clustering. The colors in the heat map represent the intensity of the log2-fold change in metabolite levels. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835451&req=5

Figure 7: Heat map illustrating the different metabolite levels in tomato plants under different treatments. Each row represents differentially expressed metabolites while each column represents a treatment. Mean values of two independent experiments are presented here. Metabolites are clustered using average linkage hierarchical clustering. The colors in the heat map represent the intensity of the log2-fold change in metabolite levels. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.
Mentions: Metabolites are considered as signaling molecules as they are associated with physiological processes. To elucidate ISR process that may be involved in PAA-mediated resistance to fusarium wilt disease, we analyzed the whole metabolome of tomato plants inoculated with the pathogen and PAA in either combination. Central metabolites changed in response to PAA + F. oxysporum, PAA, and F. oxysporum alone were normalized to respective control and were expressed in fold change (Figure 4). Here we identified more than 60 metabolites whose levels were seemed to be altered in response to different treatments (Figure 7).

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus