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Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis (PCA) score plot of metabolite finger printing of tomato shoots 7 days after treatment applications. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.
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Figure 5: Principal component analysis (PCA) score plot of metabolite finger printing of tomato shoots 7 days after treatment applications. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.

Mentions: As expected, the most represented categories included metabolites involved in defense pathways, metabolites involved in cell communication and signaling, and metabolites implicated in primary and secondary metabolism. The PCA scores plot revealed a clear separation of the all different treatments and demonstrating the significant and differential effect of the treatments on the metabolic level (Figure 5). Quantities of more than 40 metabolites appeared significantly increased or repressed in treatments receiving PAA in either combinations as compared to rest of the treatments.


Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Principal component analysis (PCA) score plot of metabolite finger printing of tomato shoots 7 days after treatment applications. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835451&req=5

Figure 5: Principal component analysis (PCA) score plot of metabolite finger printing of tomato shoots 7 days after treatment applications. FOL, F. oxysporum f.sp. lycopersici. PAA, phenylacetic acid. UC, non-treated control.
Mentions: As expected, the most represented categories included metabolites involved in defense pathways, metabolites involved in cell communication and signaling, and metabolites implicated in primary and secondary metabolism. The PCA scores plot revealed a clear separation of the all different treatments and demonstrating the significant and differential effect of the treatments on the metabolic level (Figure 5). Quantities of more than 40 metabolites appeared significantly increased or repressed in treatments receiving PAA in either combinations as compared to rest of the treatments.

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus