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Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus

Influence of root treatment with chemicals present in ISR active sub-fraction on the disease development on tomato plants after inoculation with fusarium wilt pathogen. Sterile distilled water was used as positive control. ISR eliciting sub-fraction was subjected to GC/MS analysis and chemicals present were purchased and subjected to ISR bioassay. Vertical bar represents standard errors. Asterisks indicate statistically significant reduction in disease index as compared to pathogen control as governed by ANOVA at P ≤ 0.05.
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Figure 3: Influence of root treatment with chemicals present in ISR active sub-fraction on the disease development on tomato plants after inoculation with fusarium wilt pathogen. Sterile distilled water was used as positive control. ISR eliciting sub-fraction was subjected to GC/MS analysis and chemicals present were purchased and subjected to ISR bioassay. Vertical bar represents standard errors. Asterisks indicate statistically significant reduction in disease index as compared to pathogen control as governed by ANOVA at P ≤ 0.05.

Mentions: To finally confirm the potential of ISR determinant/s, use of pure biochemicals as present in ISR active sub-fraction was focused upon. In these ISR bioassays, only PAA was found to suppress fusarium wilt (Figures 1 and 3). Thus the results indicated that 0.1 and 1.0 mM PAA significantly (P ≤ 0.05) reduced disease index up to 58.4 and 76.7%, respectively, as compared to that in the pathogen control (Figure 3). The other compounds failed to elicit significant (P ≤ 0.05) reduction in DI (Figures 1 and 3). Conspicuously, tomato plants receiving PAA provided better growth as compared to the rest of the treatments in this ISR bioassay that can be attributed toward plant growth promoting capability of PAA (Figure 1).


Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Influence of root treatment with chemicals present in ISR active sub-fraction on the disease development on tomato plants after inoculation with fusarium wilt pathogen. Sterile distilled water was used as positive control. ISR eliciting sub-fraction was subjected to GC/MS analysis and chemicals present were purchased and subjected to ISR bioassay. Vertical bar represents standard errors. Asterisks indicate statistically significant reduction in disease index as compared to pathogen control as governed by ANOVA at P ≤ 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835451&req=5

Figure 3: Influence of root treatment with chemicals present in ISR active sub-fraction on the disease development on tomato plants after inoculation with fusarium wilt pathogen. Sterile distilled water was used as positive control. ISR eliciting sub-fraction was subjected to GC/MS analysis and chemicals present were purchased and subjected to ISR bioassay. Vertical bar represents standard errors. Asterisks indicate statistically significant reduction in disease index as compared to pathogen control as governed by ANOVA at P ≤ 0.05.
Mentions: To finally confirm the potential of ISR determinant/s, use of pure biochemicals as present in ISR active sub-fraction was focused upon. In these ISR bioassays, only PAA was found to suppress fusarium wilt (Figures 1 and 3). Thus the results indicated that 0.1 and 1.0 mM PAA significantly (P ≤ 0.05) reduced disease index up to 58.4 and 76.7%, respectively, as compared to that in the pathogen control (Figure 3). The other compounds failed to elicit significant (P ≤ 0.05) reduction in DI (Figures 1 and 3). Conspicuously, tomato plants receiving PAA provided better growth as compared to the rest of the treatments in this ISR bioassay that can be attributed toward plant growth promoting capability of PAA (Figure 1).

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus