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Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus

Elucidation of biochemicals presents in ISR active sub-fraction in cell free culture filtrates of B. fortis IAGS162. (A) Chromatogram of Gas Chromatography and Mass Spectrometry analysis (GC/MS) for identification of the ISR determinant/s of B. fortis IAGS162 present in the ISR-active sub-fraction. (B) The mass spectrum analysis obtained by electrospray ionization of Phenylacetic acid.
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Figure 2: Elucidation of biochemicals presents in ISR active sub-fraction in cell free culture filtrates of B. fortis IAGS162. (A) Chromatogram of Gas Chromatography and Mass Spectrometry analysis (GC/MS) for identification of the ISR determinant/s of B. fortis IAGS162 present in the ISR-active sub-fraction. (B) The mass spectrum analysis obtained by electrospray ionization of Phenylacetic acid.

Mentions: In search for the ISR determinant/s, CFCF of B. fortis IAGS162 was extracted by using organic solvents system and ISR bioassays were performed by using extracts obtained. Compared to the rest of the treatments, chloroform fraction elicited ISR in tomato plants against fusarium wilt disease (Figure 1). Biochemcials present in this fraction were further sub-divided by performing silica gel chromatography, and the sub-fractions were again used in ISR bioassays. GC/MS analysis identified five compounds in ISR active sub-fraction including, acetic acid methyl ester, PAA, palmitic acid, propanol, and tyrosine (Figure 2).


Phenylacetic Acid Is ISR Determinant Produced by Bacillus fortis IAGS162, Which Involves Extensive Re-modulation in Metabolomics of Tomato to Protect against Fusarium Wilt.

Akram W, Anjum T, Ali B - Front Plant Sci (2016)

Elucidation of biochemicals presents in ISR active sub-fraction in cell free culture filtrates of B. fortis IAGS162. (A) Chromatogram of Gas Chromatography and Mass Spectrometry analysis (GC/MS) for identification of the ISR determinant/s of B. fortis IAGS162 present in the ISR-active sub-fraction. (B) The mass spectrum analysis obtained by electrospray ionization of Phenylacetic acid.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835451&req=5

Figure 2: Elucidation of biochemicals presents in ISR active sub-fraction in cell free culture filtrates of B. fortis IAGS162. (A) Chromatogram of Gas Chromatography and Mass Spectrometry analysis (GC/MS) for identification of the ISR determinant/s of B. fortis IAGS162 present in the ISR-active sub-fraction. (B) The mass spectrum analysis obtained by electrospray ionization of Phenylacetic acid.
Mentions: In search for the ISR determinant/s, CFCF of B. fortis IAGS162 was extracted by using organic solvents system and ISR bioassays were performed by using extracts obtained. Compared to the rest of the treatments, chloroform fraction elicited ISR in tomato plants against fusarium wilt disease (Figure 1). Biochemcials present in this fraction were further sub-divided by performing silica gel chromatography, and the sub-fractions were again used in ISR bioassays. GC/MS analysis identified five compounds in ISR active sub-fraction including, acetic acid methyl ester, PAA, palmitic acid, propanol, and tyrosine (Figure 2).

Bottom Line: Tomato plants were treated with PAA and fungal pathogen in various combinations.In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA.This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biology and Biotechnology, University of Lahore Lahore, Pakistan.

ABSTRACT
Bacillus fortis IAGS162 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the first phase of current study, the ISR determinant was isolated from extracellular metabolites of this bacterium. ISR bioassays combined with solvent extraction, column chromatography and GC/MS analysis proved that phenylacetic acid (PAA) was the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.1 and 1 mM. In the second phase, the biochemical basis of the induced systemic resistance (ISR) under influence of PAA was elucidated by performing non-targeted whole metabolomics through GC/MS analysis. Tomato plants were treated with PAA and fungal pathogen in various combinations. Exposure to PAA and subsequent pathogen challenge extensively re-modulated tomato metabolic networks along with defense related pathways. In addition, various phenylpropanoid precursors were significantly up-regulated in treatments receiving PAA. This work suggests that ISR elicitor released from B. fortis IAGS162 contributes to resistance against fungal pathogens through dynamic reprogramming of plant pathways that are functionally correlated with defense responses.

No MeSH data available.


Related in: MedlinePlus