Limits...
The CRISPR/Cas Genome-Editing Tool: Application in Improvement of Crops.

Khatodia S, Bhatotia K, Passricha N, Khurana SM, Tuteja N - Front Plant Sci (2016)

Bottom Line: The Clustered Regularly Interspaced Short Palindromic Repeats associated Cas9/sgRNA system is a novel targeted genome-editing technique derived from bacterial immune system.The regulatory uncertainty and social acceptance of plant breeding by Cas9 genome editing have also been described.With this powerful and innovative technique the designer GE non-GM plants could further advance climate resilient and sustainable agriculture in the future and maximizing yield by combating abiotic and biotic stresses.

View Article: PubMed Central - PubMed

Affiliation: Amity Institute of Biotechnology, Amity University Haryana Gurgaon, India.

ABSTRACT
The Clustered Regularly Interspaced Short Palindromic Repeats associated Cas9/sgRNA system is a novel targeted genome-editing technique derived from bacterial immune system. It is an inexpensive, easy, most user friendly and rapidly adopted genome editing tool transforming to revolutionary paradigm. This technique enables precise genomic modifications in many different organisms and tissues. Cas9 protein is an RNA guided endonuclease utilized for creating targeted double-stranded breaks with only a short RNA sequence to confer recognition of the target in animals and plants. Development of genetically edited (GE) crops similar to those developed by conventional or mutation breeding using this potential technique makes it a promising and extremely versatile tool for providing sustainable productive agriculture for better feeding of rapidly growing population in a changing climate. The emerging areas of research for the genome editing in plants include interrogating gene function, rewiring the regulatory signaling networks and sgRNA library for high-throughput loss-of-function screening. In this review, we have described the broad applicability of the Cas9 nuclease mediated targeted plant genome editing for development of designer crops. The regulatory uncertainty and social acceptance of plant breeding by Cas9 genome editing have also been described. With this powerful and innovative technique the designer GE non-GM plants could further advance climate resilient and sustainable agriculture in the future and maximizing yield by combating abiotic and biotic stresses.

No MeSH data available.


Related in: MedlinePlus

The types, applications and future possibilities of CRISPR/Cas9 system for development of GE crops for crop improvement.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835450&req=5

Figure 6: The types, applications and future possibilities of CRISPR/Cas9 system for development of GE crops for crop improvement.

Mentions: The potential future crops for sustainable productive agriculture by genome editing are those which have better pest resistance, with enhanced nutritional value, and that are able to survive in changing climate. Climate resilient agriculture for combating abiotic and biotic stress is the future of crop improvement using genome editing for both the targeted mutagenesis mediated manipulation and study of transcriptional control by dissection of physiological and molecular cross talk under combined stress (Kissoudis et al., 2014; Jain, 2015). Genome editing will play very important role in developing new bio-energy crops, which could give maximum yield on wastelands and changing climate (Bosch and Hazen, 2013). This technology could offer any possible novel genome-editing concept for plants in order to improve crops for better nutrition and food security. We here suggest some of the possible concepts, which could be utilized for crop improvement and plant biotechnology applications (Liu and Fan, 2014) (Figure 6). Further, direct delivery methods of Cas9 and gRNA using Agrobacterium and Viral replicons by using nanoparticles can be very useful for simplifying the genome editing technology (Hiei et al., 2014; Khatodia et al., 2014; Nonaka and Ezura, 2014). Inducible Cas9 system for transcription modulation like split-Cas9 for chemically inducible system and light activated Cas9 effector (LACE) could be utilized for crop improvement in future (Polstein and Gersbach, 2015; Zetsche et al., 2015). The generation of large-scale whole-genome targeted sgRNA library for high-throughput loss-of-function screening applications based on the CRISPRi system like that of RNAi is particularly feasible for model plants in future (Heintze et al., 2013).


The CRISPR/Cas Genome-Editing Tool: Application in Improvement of Crops.

Khatodia S, Bhatotia K, Passricha N, Khurana SM, Tuteja N - Front Plant Sci (2016)

The types, applications and future possibilities of CRISPR/Cas9 system for development of GE crops for crop improvement.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835450&req=5

Figure 6: The types, applications and future possibilities of CRISPR/Cas9 system for development of GE crops for crop improvement.
Mentions: The potential future crops for sustainable productive agriculture by genome editing are those which have better pest resistance, with enhanced nutritional value, and that are able to survive in changing climate. Climate resilient agriculture for combating abiotic and biotic stress is the future of crop improvement using genome editing for both the targeted mutagenesis mediated manipulation and study of transcriptional control by dissection of physiological and molecular cross talk under combined stress (Kissoudis et al., 2014; Jain, 2015). Genome editing will play very important role in developing new bio-energy crops, which could give maximum yield on wastelands and changing climate (Bosch and Hazen, 2013). This technology could offer any possible novel genome-editing concept for plants in order to improve crops for better nutrition and food security. We here suggest some of the possible concepts, which could be utilized for crop improvement and plant biotechnology applications (Liu and Fan, 2014) (Figure 6). Further, direct delivery methods of Cas9 and gRNA using Agrobacterium and Viral replicons by using nanoparticles can be very useful for simplifying the genome editing technology (Hiei et al., 2014; Khatodia et al., 2014; Nonaka and Ezura, 2014). Inducible Cas9 system for transcription modulation like split-Cas9 for chemically inducible system and light activated Cas9 effector (LACE) could be utilized for crop improvement in future (Polstein and Gersbach, 2015; Zetsche et al., 2015). The generation of large-scale whole-genome targeted sgRNA library for high-throughput loss-of-function screening applications based on the CRISPRi system like that of RNAi is particularly feasible for model plants in future (Heintze et al., 2013).

Bottom Line: The Clustered Regularly Interspaced Short Palindromic Repeats associated Cas9/sgRNA system is a novel targeted genome-editing technique derived from bacterial immune system.The regulatory uncertainty and social acceptance of plant breeding by Cas9 genome editing have also been described.With this powerful and innovative technique the designer GE non-GM plants could further advance climate resilient and sustainable agriculture in the future and maximizing yield by combating abiotic and biotic stresses.

View Article: PubMed Central - PubMed

Affiliation: Amity Institute of Biotechnology, Amity University Haryana Gurgaon, India.

ABSTRACT
The Clustered Regularly Interspaced Short Palindromic Repeats associated Cas9/sgRNA system is a novel targeted genome-editing technique derived from bacterial immune system. It is an inexpensive, easy, most user friendly and rapidly adopted genome editing tool transforming to revolutionary paradigm. This technique enables precise genomic modifications in many different organisms and tissues. Cas9 protein is an RNA guided endonuclease utilized for creating targeted double-stranded breaks with only a short RNA sequence to confer recognition of the target in animals and plants. Development of genetically edited (GE) crops similar to those developed by conventional or mutation breeding using this potential technique makes it a promising and extremely versatile tool for providing sustainable productive agriculture for better feeding of rapidly growing population in a changing climate. The emerging areas of research for the genome editing in plants include interrogating gene function, rewiring the regulatory signaling networks and sgRNA library for high-throughput loss-of-function screening. In this review, we have described the broad applicability of the Cas9 nuclease mediated targeted plant genome editing for development of designer crops. The regulatory uncertainty and social acceptance of plant breeding by Cas9 genome editing have also been described. With this powerful and innovative technique the designer GE non-GM plants could further advance climate resilient and sustainable agriculture in the future and maximizing yield by combating abiotic and biotic stresses.

No MeSH data available.


Related in: MedlinePlus