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Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization

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ABSTRACT

The present investigation focused on screening of a new potent strain for laccase production and optimizing the process parameters to achieve the maximum enzymatic decolourization of textile azo dye Congo red. Seven hydrocarbonoclastic bacterial strains were selected as positive in laccase production in solid medium using 2,6 dimethoxyphenol as an enzyme activity indicator. The best enzyme producer Pseudomonasextremorientalis BU118 showed a maximum laccase activity of about 7000 U/L of wheat bran under solid-state conditions. The influence of different concentrations of dye, enzyme, salt and various incubation times on Congo red decolourization was studied using response surface methodology to find the optimum conditions required for maximum decolourization by P.extremorientalis laccase. The enzyme exhibited a remarkable colour removal capability over a wide range of dye and salt concentrations. The above results show the potential use of this bacterial laccase in the biological treatment of the textile effluent.

No MeSH data available.


Laccase production by Pseudomonasextremorientalis BU118 grown on wheat bran-based solid medium
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Fig2: Laccase production by Pseudomonasextremorientalis BU118 grown on wheat bran-based solid medium

Mentions: The production of laccase by bacteria under solid-state cultivation is found to be economical (Muthukumarasamy and Murugan 2014). As shown in Fig. 2, laccase production by P.extremorientalis first appeared on the first day (630 U/L) and increased afterwards, peaking on the 6th day (6980U/L). This result is in agreement with that of Osma et al. (2006) and El-Batal et al. (2015), who reported high microbial laccase activities on wheat bran under solid-state fermentation. The authors pointed out that the inductive laccase capability of wheat bran may be directly related to its phenolic compounds such as ferulic, coumaric and syringic acids.Fig. 2


Pseudomonas extremorientalis BU118: a new salt-tolerant laccase-secreting bacterium with biotechnological potential in textile azo dye decolourization
Laccase production by Pseudomonasextremorientalis BU118 grown on wheat bran-based solid medium
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835423&req=5

Fig2: Laccase production by Pseudomonasextremorientalis BU118 grown on wheat bran-based solid medium
Mentions: The production of laccase by bacteria under solid-state cultivation is found to be economical (Muthukumarasamy and Murugan 2014). As shown in Fig. 2, laccase production by P.extremorientalis first appeared on the first day (630 U/L) and increased afterwards, peaking on the 6th day (6980U/L). This result is in agreement with that of Osma et al. (2006) and El-Batal et al. (2015), who reported high microbial laccase activities on wheat bran under solid-state fermentation. The authors pointed out that the inductive laccase capability of wheat bran may be directly related to its phenolic compounds such as ferulic, coumaric and syringic acids.Fig. 2

View Article: PubMed Central - PubMed

ABSTRACT

The present investigation focused on screening of a new potent strain for laccase production and optimizing the process parameters to achieve the maximum enzymatic decolourization of textile azo dye Congo red. Seven hydrocarbonoclastic bacterial strains were selected as positive in laccase production in solid medium using 2,6 dimethoxyphenol as an enzyme activity indicator. The best enzyme producer Pseudomonasextremorientalis BU118 showed a maximum laccase activity of about 7000 U/L of wheat bran under solid-state conditions. The influence of different concentrations of dye, enzyme, salt and various incubation times on Congo red decolourization was studied using response surface methodology to find the optimum conditions required for maximum decolourization by P.extremorientalis laccase. The enzyme exhibited a remarkable colour removal capability over a wide range of dye and salt concentrations. The above results show the potential use of this bacterial laccase in the biological treatment of the textile effluent.

No MeSH data available.