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Tissue plasminogen activator (tPA) attenuates propofol-induced apoptosis in developing hippocampal neurons.

Liang C, Ding M, Du F, Cang J, Xue Z - Springerplus (2016)

Bottom Line: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.Propofol decreased tPA level in the media of developing hippocampal neurons.The addition of tPA could partially reverse the apoptotic effect of propofol.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Fenglin Road 180, Shanghai, 200032 China.

ABSTRACT

Background: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.

Methods: Primary hippocampal neurons isolated from neonatal Sprague-Dawley rats were exposed to propofol (20, 50, and 100 μM) for 6 h either one time or three times. Finally, neurons were pretreated with exogenous tPA (5 µg/ml), followed by propofol exposure (100 μM, 6 h). The neuron apoptosis was detected by terminal transferase deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and the protein expression of cleaved caspase-3 (Cl-Csp3) was analyzed by western blot, the tPA in media was tested by enzyme-linked immunosorbent assay.

Results: Propofol exposure significantly increased the number of TUNEL-positive neurons and Cl-Csp3 expression in developing hippocampal neurons. Propofol decreased tPA level in the media of developing hippocampal neurons. The neuron appotosis induced by propofol was attenuated by pretreatment of tPA.

Conclusion: Propofol exposure decreased tPA release in developing hippocampal neurons. The addition of tPA could partially reverse the apoptotic effect of propofol.

No MeSH data available.


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The effects of propofol on tPA release
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Fig2: The effects of propofol on tPA release

Mentions: The neurons were exposed one and three times to 6 h of 20, 50, and 100 µM propofol. The tPA in the media was tested by ELISA. Compared with the control group, neurons exposed to propofol had less tPA in the media (P < 0.05) (Fig. 2).Fig. 2


Tissue plasminogen activator (tPA) attenuates propofol-induced apoptosis in developing hippocampal neurons.

Liang C, Ding M, Du F, Cang J, Xue Z - Springerplus (2016)

The effects of propofol on tPA release
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835406&req=5

Fig2: The effects of propofol on tPA release
Mentions: The neurons were exposed one and three times to 6 h of 20, 50, and 100 µM propofol. The tPA in the media was tested by ELISA. Compared with the control group, neurons exposed to propofol had less tPA in the media (P < 0.05) (Fig. 2).Fig. 2

Bottom Line: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.Propofol decreased tPA level in the media of developing hippocampal neurons.The addition of tPA could partially reverse the apoptotic effect of propofol.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Fenglin Road 180, Shanghai, 200032 China.

ABSTRACT

Background: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.

Methods: Primary hippocampal neurons isolated from neonatal Sprague-Dawley rats were exposed to propofol (20, 50, and 100 μM) for 6 h either one time or three times. Finally, neurons were pretreated with exogenous tPA (5 µg/ml), followed by propofol exposure (100 μM, 6 h). The neuron apoptosis was detected by terminal transferase deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and the protein expression of cleaved caspase-3 (Cl-Csp3) was analyzed by western blot, the tPA in media was tested by enzyme-linked immunosorbent assay.

Results: Propofol exposure significantly increased the number of TUNEL-positive neurons and Cl-Csp3 expression in developing hippocampal neurons. Propofol decreased tPA level in the media of developing hippocampal neurons. The neuron appotosis induced by propofol was attenuated by pretreatment of tPA.

Conclusion: Propofol exposure decreased tPA release in developing hippocampal neurons. The addition of tPA could partially reverse the apoptotic effect of propofol.

No MeSH data available.


Related in: MedlinePlus