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Tissue plasminogen activator (tPA) attenuates propofol-induced apoptosis in developing hippocampal neurons.

Liang C, Ding M, Du F, Cang J, Xue Z - Springerplus (2016)

Bottom Line: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.Propofol decreased tPA level in the media of developing hippocampal neurons.The addition of tPA could partially reverse the apoptotic effect of propofol.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Fenglin Road 180, Shanghai, 200032 China.

ABSTRACT

Background: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.

Methods: Primary hippocampal neurons isolated from neonatal Sprague-Dawley rats were exposed to propofol (20, 50, and 100 μM) for 6 h either one time or three times. Finally, neurons were pretreated with exogenous tPA (5 µg/ml), followed by propofol exposure (100 μM, 6 h). The neuron apoptosis was detected by terminal transferase deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and the protein expression of cleaved caspase-3 (Cl-Csp3) was analyzed by western blot, the tPA in media was tested by enzyme-linked immunosorbent assay.

Results: Propofol exposure significantly increased the number of TUNEL-positive neurons and Cl-Csp3 expression in developing hippocampal neurons. Propofol decreased tPA level in the media of developing hippocampal neurons. The neuron appotosis induced by propofol was attenuated by pretreatment of tPA.

Conclusion: Propofol exposure decreased tPA release in developing hippocampal neurons. The addition of tPA could partially reverse the apoptotic effect of propofol.

No MeSH data available.


Related in: MedlinePlus

Propofol induces appotosis in cultured developing hippocampal neurons. The neurons were exposed one or three times to 6 h of 20, 50, and 100 µM propofol. After treatments, the TUNEL-positive neurons and the protein expression of Cl-Csp3 were analyzed by TUNEL staining (a) and western blot (b), respectively. Densitometric analysis of Cl-Csp3 protein was performed after normalization with β-actin (b). *,▲P < 0.05 vs C1 or C2. C1 control group of single exposure to propofol, C2 control group of multiple exposure to propofol
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Fig1: Propofol induces appotosis in cultured developing hippocampal neurons. The neurons were exposed one or three times to 6 h of 20, 50, and 100 µM propofol. After treatments, the TUNEL-positive neurons and the protein expression of Cl-Csp3 were analyzed by TUNEL staining (a) and western blot (b), respectively. Densitometric analysis of Cl-Csp3 protein was performed after normalization with β-actin (b). *,▲P < 0.05 vs C1 or C2. C1 control group of single exposure to propofol, C2 control group of multiple exposure to propofol

Mentions: The neurons were exposed one and three times to 6 h of 20, 50, and 100 µM propofol. The number of TUNEL-positive neurons in propofol-treated group was significantly increased when compared with these of control group (P < 0.05), following one exposure to 50 and 100 µM propofol but not after a single exposure to 20 µM propofol (P < 0.05) (Fig. 1a). The number of TUNEL-positive neurons was significantly increased in all propofol-treated groups after three exposures to propofol (P < 0.05) (Fig. 1a). Consistently, the protein expression levels of the apoptosis executor cleaved caspase-3 (Cl-Csp3) were also significantly increased by propofol exposure (P < 0.05) (Fig. 1b).Fig. 1


Tissue plasminogen activator (tPA) attenuates propofol-induced apoptosis in developing hippocampal neurons.

Liang C, Ding M, Du F, Cang J, Xue Z - Springerplus (2016)

Propofol induces appotosis in cultured developing hippocampal neurons. The neurons were exposed one or three times to 6 h of 20, 50, and 100 µM propofol. After treatments, the TUNEL-positive neurons and the protein expression of Cl-Csp3 were analyzed by TUNEL staining (a) and western blot (b), respectively. Densitometric analysis of Cl-Csp3 protein was performed after normalization with β-actin (b). *,▲P < 0.05 vs C1 or C2. C1 control group of single exposure to propofol, C2 control group of multiple exposure to propofol
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835406&req=5

Fig1: Propofol induces appotosis in cultured developing hippocampal neurons. The neurons were exposed one or three times to 6 h of 20, 50, and 100 µM propofol. After treatments, the TUNEL-positive neurons and the protein expression of Cl-Csp3 were analyzed by TUNEL staining (a) and western blot (b), respectively. Densitometric analysis of Cl-Csp3 protein was performed after normalization with β-actin (b). *,▲P < 0.05 vs C1 or C2. C1 control group of single exposure to propofol, C2 control group of multiple exposure to propofol
Mentions: The neurons were exposed one and three times to 6 h of 20, 50, and 100 µM propofol. The number of TUNEL-positive neurons in propofol-treated group was significantly increased when compared with these of control group (P < 0.05), following one exposure to 50 and 100 µM propofol but not after a single exposure to 20 µM propofol (P < 0.05) (Fig. 1a). The number of TUNEL-positive neurons was significantly increased in all propofol-treated groups after three exposures to propofol (P < 0.05) (Fig. 1a). Consistently, the protein expression levels of the apoptosis executor cleaved caspase-3 (Cl-Csp3) were also significantly increased by propofol exposure (P < 0.05) (Fig. 1b).Fig. 1

Bottom Line: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.Propofol decreased tPA level in the media of developing hippocampal neurons.The addition of tPA could partially reverse the apoptotic effect of propofol.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, Zhongshan Hospital, Fudan University, Fenglin Road 180, Shanghai, 200032 China.

ABSTRACT

Background: We investigated the effect of propofol on the tissue plasminogen activator (tPA) release in developing hippocampal neurons, and explored the effects of exogenous tPA on the propofol-induced neuron apoptosis.

Methods: Primary hippocampal neurons isolated from neonatal Sprague-Dawley rats were exposed to propofol (20, 50, and 100 μM) for 6 h either one time or three times. Finally, neurons were pretreated with exogenous tPA (5 µg/ml), followed by propofol exposure (100 μM, 6 h). The neuron apoptosis was detected by terminal transferase deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) and the protein expression of cleaved caspase-3 (Cl-Csp3) was analyzed by western blot, the tPA in media was tested by enzyme-linked immunosorbent assay.

Results: Propofol exposure significantly increased the number of TUNEL-positive neurons and Cl-Csp3 expression in developing hippocampal neurons. Propofol decreased tPA level in the media of developing hippocampal neurons. The neuron appotosis induced by propofol was attenuated by pretreatment of tPA.

Conclusion: Propofol exposure decreased tPA release in developing hippocampal neurons. The addition of tPA could partially reverse the apoptotic effect of propofol.

No MeSH data available.


Related in: MedlinePlus