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Conventional and modified hydrodistillation method for the extraction of glucosinolate hydrolytic products: a comparative account.

Arora R, Singh B, Vig AP, Arora S - Springerplus (2016)

Bottom Line: Eruca sativa is extensively used as raw and its oil is also used for cooking due to its exceptional flavour.The volatile nature of the hydrolytic products of glucosinolates makes the extraction difficult.The yield and composition of hydrolytic products in the extract with the modified method was increased along with an increase in the amount of major hydrolytic products as seen by GC-MS.

View Article: PubMed Central - PubMed

Affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.

ABSTRACT
Eruca sativa is extensively used as raw and its oil is also used for cooking due to its exceptional flavour. The volatile nature of the hydrolytic products of glucosinolates makes the extraction difficult. The hydrodistillation method used previously yield very less amount of the extract as well as the absence of stirring in the round bottom flask causes burning of both the crushed seeds and the flask. To overcome these drawbacks, a method has been developed using magnetic stirrer and hot plate. The yield and composition of hydrolytic products in the extract with the modified method was increased along with an increase in the amount of major hydrolytic products as seen by GC-MS. This method thus has immense potential in pharmaceutical industries, due to the ease of extraction and isolation.

No MeSH data available.


Related in: MedlinePlus

The different hydrodistillations methods where a conventional method and b modified method
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Fig1: The different hydrodistillations methods where a conventional method and b modified method

Mentions: The conventional hydrodistillation was done following the method given by Blazevic and Mastelic (Blazevic and Mastelic 2009). In this, 50 g seeds of Eruca sativa (Mill.) Thell. var. RTM-1212 (procured from Sri Karan Narendra College of Agriculture, Jobner, Rajasthan, India), were crushed and added to 1000 ml distilled water (DW) in a round bottom flask. The flask was then kept in a heating mantle and the Clevenger apparatus was attached (Fig. 1a). The mixture was allowed to boil at 100 °C and then the temperature was reduced to 60 °C and kept for 3 h. The oil and water obtained was collected in a separation flask and the GHPs were obtained by fractionation using AR grade dichloromethane (DCM). The solvent was passed through anhydrous sodium sulphate and evaporated under vacuum at 30 °C, using rotary evaporator (Buchi R210). It was redissolved in GC grade DCM and passed through 0.22 μm filter prior to GC–MS injection.Fig. 1


Conventional and modified hydrodistillation method for the extraction of glucosinolate hydrolytic products: a comparative account.

Arora R, Singh B, Vig AP, Arora S - Springerplus (2016)

The different hydrodistillations methods where a conventional method and b modified method
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835399&req=5

Fig1: The different hydrodistillations methods where a conventional method and b modified method
Mentions: The conventional hydrodistillation was done following the method given by Blazevic and Mastelic (Blazevic and Mastelic 2009). In this, 50 g seeds of Eruca sativa (Mill.) Thell. var. RTM-1212 (procured from Sri Karan Narendra College of Agriculture, Jobner, Rajasthan, India), were crushed and added to 1000 ml distilled water (DW) in a round bottom flask. The flask was then kept in a heating mantle and the Clevenger apparatus was attached (Fig. 1a). The mixture was allowed to boil at 100 °C and then the temperature was reduced to 60 °C and kept for 3 h. The oil and water obtained was collected in a separation flask and the GHPs were obtained by fractionation using AR grade dichloromethane (DCM). The solvent was passed through anhydrous sodium sulphate and evaporated under vacuum at 30 °C, using rotary evaporator (Buchi R210). It was redissolved in GC grade DCM and passed through 0.22 μm filter prior to GC–MS injection.Fig. 1

Bottom Line: Eruca sativa is extensively used as raw and its oil is also used for cooking due to its exceptional flavour.The volatile nature of the hydrolytic products of glucosinolates makes the extraction difficult.The yield and composition of hydrolytic products in the extract with the modified method was increased along with an increase in the amount of major hydrolytic products as seen by GC-MS.

View Article: PubMed Central - PubMed

Affiliation: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005 India.

ABSTRACT
Eruca sativa is extensively used as raw and its oil is also used for cooking due to its exceptional flavour. The volatile nature of the hydrolytic products of glucosinolates makes the extraction difficult. The hydrodistillation method used previously yield very less amount of the extract as well as the absence of stirring in the round bottom flask causes burning of both the crushed seeds and the flask. To overcome these drawbacks, a method has been developed using magnetic stirrer and hot plate. The yield and composition of hydrolytic products in the extract with the modified method was increased along with an increase in the amount of major hydrolytic products as seen by GC-MS. This method thus has immense potential in pharmaceutical industries, due to the ease of extraction and isolation.

No MeSH data available.


Related in: MedlinePlus