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Assessing the geographic scale of genetic population management with microsatellites and introns in the clam Ruditapes decussatus.

Arias-Pérez A, Cordero D, Borrell Y, Sánchez JA, Blanco G, Freire R, Insua A, Saavedra C - Ecol Evol (2016)

Bottom Line: Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations.Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean.This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioloxía Celular e Molecular Universidade da Coruña A Zapateira s/n 15071 A Coruña Spain.

ABSTRACT
The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila clam (R. philippinarum). Previous studies with intronic markers showed a genetic subdivision of the species in three races (Atlantic, West Mediterranean, and Adriatic-Aegean). However, detailed population genetic studies to help management of the main production areas in the southwest of Europe are missing. We have analyzed eight Atlantic and two Mediterranean populations from the Spanish coasts using 14 microsatellites and six intronic markers. Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations. Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean. This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

No MeSH data available.


Related in: MedlinePlus

Tests for isolation by distance. (A) and (C) include all samples, and (B) and (D) Atlantic populations only. The charts on the left are based on intronic data and those in the right are based on microsatellite data.
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ece32052-fig-0006: Tests for isolation by distance. (A) and (C) include all samples, and (B) and (D) Atlantic populations only. The charts on the left are based on intronic data and those in the right are based on microsatellite data.

Mentions: No significant correlation was observed between geographic distance and the test statistic for an IBD model for intronic markers (r = 0.518, P = 0.075) (Fig. 6). Correlation was even lower when only the Atlantic populations were taken in account (r = 0.017, P = 0.395). However, for microsatellites, the Mantel test indicated that the degree of genetic differentiation increased significantly with distance (r = 0.704, P = 0.002) indicating support for an isolation by distance (IBD) model (Fig. 6). When the two Mediterranean samples were removed from the analysis, an IBD model continued to be supported for the remaining samples, showing even a higher correlation (r = 0.886, P < 0.001).


Assessing the geographic scale of genetic population management with microsatellites and introns in the clam Ruditapes decussatus.

Arias-Pérez A, Cordero D, Borrell Y, Sánchez JA, Blanco G, Freire R, Insua A, Saavedra C - Ecol Evol (2016)

Tests for isolation by distance. (A) and (C) include all samples, and (B) and (D) Atlantic populations only. The charts on the left are based on intronic data and those in the right are based on microsatellite data.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835341&req=5

ece32052-fig-0006: Tests for isolation by distance. (A) and (C) include all samples, and (B) and (D) Atlantic populations only. The charts on the left are based on intronic data and those in the right are based on microsatellite data.
Mentions: No significant correlation was observed between geographic distance and the test statistic for an IBD model for intronic markers (r = 0.518, P = 0.075) (Fig. 6). Correlation was even lower when only the Atlantic populations were taken in account (r = 0.017, P = 0.395). However, for microsatellites, the Mantel test indicated that the degree of genetic differentiation increased significantly with distance (r = 0.704, P = 0.002) indicating support for an isolation by distance (IBD) model (Fig. 6). When the two Mediterranean samples were removed from the analysis, an IBD model continued to be supported for the remaining samples, showing even a higher correlation (r = 0.886, P < 0.001).

Bottom Line: Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations.Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean.This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioloxía Celular e Molecular Universidade da Coruña A Zapateira s/n 15071 A Coruña Spain.

ABSTRACT
The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila clam (R. philippinarum). Previous studies with intronic markers showed a genetic subdivision of the species in three races (Atlantic, West Mediterranean, and Adriatic-Aegean). However, detailed population genetic studies to help management of the main production areas in the southwest of Europe are missing. We have analyzed eight Atlantic and two Mediterranean populations from the Spanish coasts using 14 microsatellites and six intronic markers. Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations. Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean. This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

No MeSH data available.


Related in: MedlinePlus