Limits...
Assessing the geographic scale of genetic population management with microsatellites and introns in the clam Ruditapes decussatus.

Arias-Pérez A, Cordero D, Borrell Y, Sánchez JA, Blanco G, Freire R, Insua A, Saavedra C - Ecol Evol (2016)

Bottom Line: Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations.Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean.This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioloxía Celular e Molecular Universidade da Coruña A Zapateira s/n 15071 A Coruña Spain.

ABSTRACT
The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila clam (R. philippinarum). Previous studies with intronic markers showed a genetic subdivision of the species in three races (Atlantic, West Mediterranean, and Adriatic-Aegean). However, detailed population genetic studies to help management of the main production areas in the southwest of Europe are missing. We have analyzed eight Atlantic and two Mediterranean populations from the Spanish coasts using 14 microsatellites and six intronic markers. Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations. Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean. This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

No MeSH data available.


Related in: MedlinePlus

Bayesian analysis of genetic structure from intronic (A and B) and microsatellite (C and D) data. (A and C) Distribution of the Estimated log Likelihood of K, L(K). (B and D) ΔK as a function of K. For L(K) each point corresponds to the mean L(K) ± SD across 20 independent runs.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835341&req=5

ece32052-fig-0003: Bayesian analysis of genetic structure from intronic (A and B) and microsatellite (C and D) data. (A and C) Distribution of the Estimated log Likelihood of K, L(K). (B and D) ΔK as a function of K. For L(K) each point corresponds to the mean L(K) ± SD across 20 independent runs.

Mentions: Figures 3 and 4 show the results of the Bayesian clustering analysis for three different number of clusters (K). Overall Obstruct R2 values for K = 2–4 varied between 0.91 and 0.98 and were highly significant (P < 0.001), indicating strong correlation between inferred ancestries and predefined populations and therefore strong population structure. The values of ln P (X/K Pritchard et al. (2000) and ΔK (Evanno et al. 2005) were highest for K = 3 (Fig. 3), but high values at K = 4 indicated additional structure in the Atlantic. A model with K = 2 showed two clusters that clearly divided samples belonging to the Mediterranean from the Atlantic samples, according to the differences in cluster frequencies. With K = 3, the populations from Rías Baixas were distinguished from the remaining Atlantic, and with K = 4, four geographic regions could be distinguished: Cantabrian Sea, Rías Baixas, Gulf of Cadiz, and Mediterranean (Fig. 4).


Assessing the geographic scale of genetic population management with microsatellites and introns in the clam Ruditapes decussatus.

Arias-Pérez A, Cordero D, Borrell Y, Sánchez JA, Blanco G, Freire R, Insua A, Saavedra C - Ecol Evol (2016)

Bayesian analysis of genetic structure from intronic (A and B) and microsatellite (C and D) data. (A and C) Distribution of the Estimated log Likelihood of K, L(K). (B and D) ΔK as a function of K. For L(K) each point corresponds to the mean L(K) ± SD across 20 independent runs.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835341&req=5

ece32052-fig-0003: Bayesian analysis of genetic structure from intronic (A and B) and microsatellite (C and D) data. (A and C) Distribution of the Estimated log Likelihood of K, L(K). (B and D) ΔK as a function of K. For L(K) each point corresponds to the mean L(K) ± SD across 20 independent runs.
Mentions: Figures 3 and 4 show the results of the Bayesian clustering analysis for three different number of clusters (K). Overall Obstruct R2 values for K = 2–4 varied between 0.91 and 0.98 and were highly significant (P < 0.001), indicating strong correlation between inferred ancestries and predefined populations and therefore strong population structure. The values of ln P (X/K Pritchard et al. (2000) and ΔK (Evanno et al. 2005) were highest for K = 3 (Fig. 3), but high values at K = 4 indicated additional structure in the Atlantic. A model with K = 2 showed two clusters that clearly divided samples belonging to the Mediterranean from the Atlantic samples, according to the differences in cluster frequencies. With K = 3, the populations from Rías Baixas were distinguished from the remaining Atlantic, and with K = 4, four geographic regions could be distinguished: Cantabrian Sea, Rías Baixas, Gulf of Cadiz, and Mediterranean (Fig. 4).

Bottom Line: Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations.Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean.This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioloxía Celular e Molecular Universidade da Coruña A Zapateira s/n 15071 A Coruña Spain.

ABSTRACT
The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila clam (R. philippinarum). Previous studies with intronic markers showed a genetic subdivision of the species in three races (Atlantic, West Mediterranean, and Adriatic-Aegean). However, detailed population genetic studies to help management of the main production areas in the southwest of Europe are missing. We have analyzed eight Atlantic and two Mediterranean populations from the Spanish coasts using 14 microsatellites and six intronic markers. Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations. Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean. This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

No MeSH data available.


Related in: MedlinePlus