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Assessing the geographic scale of genetic population management with microsatellites and introns in the clam Ruditapes decussatus.

Arias-Pérez A, Cordero D, Borrell Y, Sánchez JA, Blanco G, Freire R, Insua A, Saavedra C - Ecol Evol (2016)

Bottom Line: Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations.Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean.This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioloxía Celular e Molecular Universidade da Coruña A Zapateira s/n 15071 A Coruña Spain.

ABSTRACT
The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila clam (R. philippinarum). Previous studies with intronic markers showed a genetic subdivision of the species in three races (Atlantic, West Mediterranean, and Adriatic-Aegean). However, detailed population genetic studies to help management of the main production areas in the southwest of Europe are missing. We have analyzed eight Atlantic and two Mediterranean populations from the Spanish coasts using 14 microsatellites and six intronic markers. Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations. Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean. This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

No MeSH data available.


Related in: MedlinePlus

Map showing the localities sampled in this study. Car: Carasa; Pon: Pontejos; Vil: Villaviciosa; Eo: Ría del Eo; Cam: Cambados; Red: Redondela; Isl: Isla Cristina; Rio: Río Piedras; Mur: Murcia; Del: Ebro Delta.
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ece32052-fig-0001: Map showing the localities sampled in this study. Car: Carasa; Pon: Pontejos; Vil: Villaviciosa; Eo: Ría del Eo; Cam: Cambados; Red: Redondela; Isl: Isla Cristina; Rio: Río Piedras; Mur: Murcia; Del: Ebro Delta.

Mentions: A total of 513 individuals were collected from 10 Spanish natural populations distributed in four coastal regions: Cantabrian Sea, Rías Baixas, Gulf of Cadiz, and Mediterranean Sea (Fig. 1). Data for two samples, Eo and Vil, were the same used in Borrell et al. (2014). Genomic DNA was obtained from a small piece of adductor muscle using the Zymobead TM Genomic DNA Kit (Zymo Research Corp., Irvine, CA), the method of Fernández‐Tajes and Méndez (2007), or by boiling during 20 min in a 10% preparation of the cation exchange resin Chelex 100, 200–400 mesh (Bio‐Rad, Hercules, CA).


Assessing the geographic scale of genetic population management with microsatellites and introns in the clam Ruditapes decussatus.

Arias-Pérez A, Cordero D, Borrell Y, Sánchez JA, Blanco G, Freire R, Insua A, Saavedra C - Ecol Evol (2016)

Map showing the localities sampled in this study. Car: Carasa; Pon: Pontejos; Vil: Villaviciosa; Eo: Ría del Eo; Cam: Cambados; Red: Redondela; Isl: Isla Cristina; Rio: Río Piedras; Mur: Murcia; Del: Ebro Delta.
© Copyright Policy - creativeCommonsBy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835341&req=5

ece32052-fig-0001: Map showing the localities sampled in this study. Car: Carasa; Pon: Pontejos; Vil: Villaviciosa; Eo: Ría del Eo; Cam: Cambados; Red: Redondela; Isl: Isla Cristina; Rio: Río Piedras; Mur: Murcia; Del: Ebro Delta.
Mentions: A total of 513 individuals were collected from 10 Spanish natural populations distributed in four coastal regions: Cantabrian Sea, Rías Baixas, Gulf of Cadiz, and Mediterranean Sea (Fig. 1). Data for two samples, Eo and Vil, were the same used in Borrell et al. (2014). Genomic DNA was obtained from a small piece of adductor muscle using the Zymobead TM Genomic DNA Kit (Zymo Research Corp., Irvine, CA), the method of Fernández‐Tajes and Méndez (2007), or by boiling during 20 min in a 10% preparation of the cation exchange resin Chelex 100, 200–400 mesh (Bio‐Rad, Hercules, CA).

Bottom Line: Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations.Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean.This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioloxía Celular e Molecular Universidade da Coruña A Zapateira s/n 15071 A Coruña Spain.

ABSTRACT
The clam Ruditapes decussatus is commercially important in southwestern Europe, suffering from population decline and hybridization with exotic Manila clam (R. philippinarum). Previous studies with intronic markers showed a genetic subdivision of the species in three races (Atlantic, West Mediterranean, and Adriatic-Aegean). However, detailed population genetic studies to help management of the main production areas in the southwest of Europe are missing. We have analyzed eight Atlantic and two Mediterranean populations from the Spanish coasts using 14 microsatellites and six intronic markers. Microsatellites confirmed the Atlantic and West Mediterranean races detected with introns and showed that genetic variability was higher in Mediterranean than in Atlantic populations. Both marker types showed that genetic differentiation of Atlantic populations was low and indicated that populations could be managed at the regional level in the case of Cantabrian and Gulf of Cadiz areas, but not in the case of Rias Baixas and the Mediterranean. This study shows the interest of including different types of markers in studies of genetic population structure of marine organisms.

No MeSH data available.


Related in: MedlinePlus