Limits...
Protective Effect of Puerarin Against Oxidative Stress Injury of Neural Cells and Related Mechanisms.

Cheng Y, Leng W, Zhang J - Med. Sci. Monit. (2016)

Bottom Line: Cell apoptosis was determined by Annexin-V/7-AAD double labelling.Reactive oxidative species (ROS) and lactate dehydrogenase (LDH) activities were then measured.Cellular levels of caspase-3 and caspase-9 were also determined.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China (mainland).

ABSTRACT
BACKGROUND Parkinson's disease (PD) is manifested as degeneration of dopaminergic neurons in substantia nigra compacta. The mitochondrial dysfunction induced by oxidative stress is believed to a major cause of PD. Puerarin has been widely applied due to its estrogen nature and anti-oxidative function. This study thus investigated the protective role of puerarin against oxidative stress injury on PC12 neural cells, in addition to related mechanisms. MATERIAL AND METHODS PC12 cells were pre-treated with gradient concentrations of puerarin, followed by the induction of 0.5 mM H2O2. MTT assay was used to detect cell viability. Enzyme-linked immunosorbent assay (ELISA) was employed to detect intracellular level of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH). Cell apoptosis was determined by Annexin-V/7-AAD double labelling. Reactive oxidative species (ROS) and lactate dehydrogenase (LDH) activities were then measured. Cellular levels of caspase-3 and caspase-9 were also determined. RESULTS The pre-treatment using puerarin significantly reversed H2O2-induced oxidative stress injury, as it can increase proliferation, SOD and GSH activities, decrease MDA activity, suppress apoptosis of PC12 cells, and decrease ROS and LDH production (p<0.05 in all cases). Further assays showed depressed up-regulation of caspase-3 and caspase-9 after puerarin pretreatment. CONCLUSIONS Puerarin pretreatment can decrease activity of caspase-3 and caspase-9 activity in PC12 cells, thus protecting cells from oxidative injury.

No MeSH data available.


Related in: MedlinePlus

ROS level and LDH production in PC 12 cells. ** p<0.01 compared to control group; ## p<0.01 compared to H2O2 group.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4835157&req=5

f3-medscimonit-22-1244: ROS level and LDH production in PC 12 cells. ** p<0.01 compared to control group; ## p<0.01 compared to H2O2 group.

Mentions: Figure 3 shows the significantly elevated ROS and LDH production in PC12 cells after H2O2 induction (p<0.01). The pre-treatment using 100 mg/L puerarin, however, significantly depressed ROS and LDH levels (p<0.01).


Protective Effect of Puerarin Against Oxidative Stress Injury of Neural Cells and Related Mechanisms.

Cheng Y, Leng W, Zhang J - Med. Sci. Monit. (2016)

ROS level and LDH production in PC 12 cells. ** p<0.01 compared to control group; ## p<0.01 compared to H2O2 group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4835157&req=5

f3-medscimonit-22-1244: ROS level and LDH production in PC 12 cells. ** p<0.01 compared to control group; ## p<0.01 compared to H2O2 group.
Mentions: Figure 3 shows the significantly elevated ROS and LDH production in PC12 cells after H2O2 induction (p<0.01). The pre-treatment using 100 mg/L puerarin, however, significantly depressed ROS and LDH levels (p<0.01).

Bottom Line: Cell apoptosis was determined by Annexin-V/7-AAD double labelling.Reactive oxidative species (ROS) and lactate dehydrogenase (LDH) activities were then measured.Cellular levels of caspase-3 and caspase-9 were also determined.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, The Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China (mainland).

ABSTRACT
BACKGROUND Parkinson's disease (PD) is manifested as degeneration of dopaminergic neurons in substantia nigra compacta. The mitochondrial dysfunction induced by oxidative stress is believed to a major cause of PD. Puerarin has been widely applied due to its estrogen nature and anti-oxidative function. This study thus investigated the protective role of puerarin against oxidative stress injury on PC12 neural cells, in addition to related mechanisms. MATERIAL AND METHODS PC12 cells were pre-treated with gradient concentrations of puerarin, followed by the induction of 0.5 mM H2O2. MTT assay was used to detect cell viability. Enzyme-linked immunosorbent assay (ELISA) was employed to detect intracellular level of superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH). Cell apoptosis was determined by Annexin-V/7-AAD double labelling. Reactive oxidative species (ROS) and lactate dehydrogenase (LDH) activities were then measured. Cellular levels of caspase-3 and caspase-9 were also determined. RESULTS The pre-treatment using puerarin significantly reversed H2O2-induced oxidative stress injury, as it can increase proliferation, SOD and GSH activities, decrease MDA activity, suppress apoptosis of PC12 cells, and decrease ROS and LDH production (p<0.05 in all cases). Further assays showed depressed up-regulation of caspase-3 and caspase-9 after puerarin pretreatment. CONCLUSIONS Puerarin pretreatment can decrease activity of caspase-3 and caspase-9 activity in PC12 cells, thus protecting cells from oxidative injury.

No MeSH data available.


Related in: MedlinePlus