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Identification of HSPA8 as a candidate biomarker for endometrial carcinoma by using iTRAQ-based proteomic analysis.

Shan N, Zhou W, Zhang S, Zhang Y - Onco Targets Ther (2016)

Bottom Line: Totally, we screened 1,266 proteins.We further validated the overexpression of HSPA8 by using immunoblot analysis.The depletion of HSPA8 siRNAs significantly reduced cell proliferation, promoted cell apoptosis, and suppressed cell growth in both cell lines.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetric and Gynecology, Central South University, Changsha, Hunan, People's Republic of China.

ABSTRACT
Although there are advances in diagnostic, predictive, and therapeutic strategies, discovering protein biomarker for early detection is required for improving the survival rate of the patients with endometrial carcinoma. In this study, we identify proteins that are differentially expressed between the Stage I endometrial carcinoma and the normal pericarcinous tissues by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. Totally, we screened 1,266 proteins. Among them, 103 proteins were significantly overexpressed, and 30 were significantly downexpressed in endometrial carcinoma. Using the bioinformatics analysis, we identified a list of proteins that might be closely associated with endometrial carcinoma, including CCT7, HSPA8, PCBP2, LONP1, PFN1, and EEF2. We validated the gene overexpression of these molecules in the endometrial carcinoma tissues and found that HSPA8 was most significantly upregulated. We further validated the overexpression of HSPA8 by using immunoblot analysis. Then, HSPA8 siRNA was transferred into the endometrial cancer cells RL-95-2 and HEC-1B. The depletion of HSPA8 siRNAs significantly reduced cell proliferation, promoted cell apoptosis, and suppressed cell growth in both cell lines. Taken together, HSPA8 plays a vital role in the development of endometrial carcinoma. HSPA8 is a candidate biomarker for early diagnosis and therapy of Stage I endometrial carcinoma.

No MeSH data available.


Related in: MedlinePlus

Classification of the identified proteins by GO database.Notes: (A) Cellular component, (B) molecular function, and (C) biological process.Abbreviation: GO, gene ontology.
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f1-ott-9-2169: Classification of the identified proteins by GO database.Notes: (A) Cellular component, (B) molecular function, and (C) biological process.Abbreviation: GO, gene ontology.

Mentions: To understand the functions of differentially expressed proteins, Gene Ontology enrichment analysis was performed to analyze the functions of these proteins in the following three categories: biological processes, molecular functions, and cellular components (Figure 1). We found six significant biological processes associated with these differentially expressed proteins (Figure 1A), including response to metabolic process (30.8%), cellular process (23.1%), immune system process (15.4%), stimulus (15.4%), biological regulation (7.7%), and localization (7.7%) and nine molecular functions (Figure 1B) associated with them, including binding (31.7%), catalytic activity (36.6%), structural molecule activity (17.1%), nucleic acid-binding transcription factor activity (3.7%), translation regulator activity (3.7%), enzyme regulator activity (2.4%), transporter activity (2.4%), receptor activity (1.2%), and antioxidant activity (1.2%). Cell part (50%) and organelle (50%) were the enriched cellular components (Figure 1C).


Identification of HSPA8 as a candidate biomarker for endometrial carcinoma by using iTRAQ-based proteomic analysis.

Shan N, Zhou W, Zhang S, Zhang Y - Onco Targets Ther (2016)

Classification of the identified proteins by GO database.Notes: (A) Cellular component, (B) molecular function, and (C) biological process.Abbreviation: GO, gene ontology.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835145&req=5

f1-ott-9-2169: Classification of the identified proteins by GO database.Notes: (A) Cellular component, (B) molecular function, and (C) biological process.Abbreviation: GO, gene ontology.
Mentions: To understand the functions of differentially expressed proteins, Gene Ontology enrichment analysis was performed to analyze the functions of these proteins in the following three categories: biological processes, molecular functions, and cellular components (Figure 1). We found six significant biological processes associated with these differentially expressed proteins (Figure 1A), including response to metabolic process (30.8%), cellular process (23.1%), immune system process (15.4%), stimulus (15.4%), biological regulation (7.7%), and localization (7.7%) and nine molecular functions (Figure 1B) associated with them, including binding (31.7%), catalytic activity (36.6%), structural molecule activity (17.1%), nucleic acid-binding transcription factor activity (3.7%), translation regulator activity (3.7%), enzyme regulator activity (2.4%), transporter activity (2.4%), receptor activity (1.2%), and antioxidant activity (1.2%). Cell part (50%) and organelle (50%) were the enriched cellular components (Figure 1C).

Bottom Line: Totally, we screened 1,266 proteins.We further validated the overexpression of HSPA8 by using immunoblot analysis.The depletion of HSPA8 siRNAs significantly reduced cell proliferation, promoted cell apoptosis, and suppressed cell growth in both cell lines.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetric and Gynecology, Central South University, Changsha, Hunan, People's Republic of China.

ABSTRACT
Although there are advances in diagnostic, predictive, and therapeutic strategies, discovering protein biomarker for early detection is required for improving the survival rate of the patients with endometrial carcinoma. In this study, we identify proteins that are differentially expressed between the Stage I endometrial carcinoma and the normal pericarcinous tissues by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. Totally, we screened 1,266 proteins. Among them, 103 proteins were significantly overexpressed, and 30 were significantly downexpressed in endometrial carcinoma. Using the bioinformatics analysis, we identified a list of proteins that might be closely associated with endometrial carcinoma, including CCT7, HSPA8, PCBP2, LONP1, PFN1, and EEF2. We validated the gene overexpression of these molecules in the endometrial carcinoma tissues and found that HSPA8 was most significantly upregulated. We further validated the overexpression of HSPA8 by using immunoblot analysis. Then, HSPA8 siRNA was transferred into the endometrial cancer cells RL-95-2 and HEC-1B. The depletion of HSPA8 siRNAs significantly reduced cell proliferation, promoted cell apoptosis, and suppressed cell growth in both cell lines. Taken together, HSPA8 plays a vital role in the development of endometrial carcinoma. HSPA8 is a candidate biomarker for early diagnosis and therapy of Stage I endometrial carcinoma.

No MeSH data available.


Related in: MedlinePlus