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Metastasis-associated lung adenocarcinoma transcript 1 promotes the proliferation of chondrosarcoma cell via activating Notch-1 signaling pathway.

Xu F, Zhang ZQ, Fang YC, Li XL, Sun Y, Xiong CZ, Yan LQ, Wang Q - Onco Targets Ther (2016)

Bottom Line: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is identified to be overexpressed in several cancers.A subcutaneous chondrosarcoma cells xenograft model was used to confirm the effect of MALAT-1 on tumor growth in vivo.Finally, we found that MALAT-1 promoted the tumor growth in a subcutaneous chondrosarcoma cells xenograft model, which confirmed the promoted effect of MALAT-1 on the tumor growth in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Hongquan Hospital, Yangzhou, Jiangsu Province, People's Republic of China.

ABSTRACT

Background: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is identified to be overexpressed in several cancers. However, the role of MALAT-1 in chondrosarcoma is poorly understood.

Methods: The expression of MALAT-1 and Notch-1 signaling pathway was detected in chondrosarcoma tissues and chondrosarcoma cells by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was performed to examine the cell viability of chondrosarcoma cells transfected with si-MALAT-1 or pcDNA-MALAT-1. Then the expression of Notch-1 signaling pathway was detected when MALAT-1 was upregulated or downregulated in chondrosarcoma cells. A subcutaneous chondrosarcoma cells xenograft model was used to confirm the effect of MALAT-1 on tumor growth in vivo.

Results: We found the increased expression of MALAT-1 and Notch-1 signaling pathway in chondrosarcoma tissue and cells. MALAT-1 promoted the proliferation of chondrosarcoma cells. In addition, MALAT-1 activated the Notch-1 signaling pathway at posttranscriptional level in chondrosarcoma cells. Meanwhile, overexpression of Notch-1 reversed the effect of si-MALAT-1 on the proliferation of chondrosarcoma cells. Finally, we found that MALAT-1 promoted the tumor growth in a subcutaneous chondrosarcoma cells xenograft model, which confirmed the promoted effect of MALAT-1 on the tumor growth in vivo.

Conclusion: Taken together, our study demonstrated that MALAT-1 promoted the proliferation of chondrosarcoma cell via activating Notch-1 signaling pathway.

No MeSH data available.


Related in: MedlinePlus

The effect of MALAT-1 on the tumor growth in nude mice.Notes: (A) The tumor growth in nude mice (six mice per group) inoculated with JJ012 cells treated with si-MALAT-1 or si-control. (B) The tumor growth in nude mice (six mice per group) inoculated with CH2879 cells treated with pcDNA-MALAT-1 or pcDNA. Each value represents the mean ± SD of triplicate wells. *P<0.05, vs control; **P<0.01, vs control.Abbreviations: MALAT-1, metastasis-associated lung adenocarcinoma transcript 1; SD, standard deviation.
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f6-ott-9-2143: The effect of MALAT-1 on the tumor growth in nude mice.Notes: (A) The tumor growth in nude mice (six mice per group) inoculated with JJ012 cells treated with si-MALAT-1 or si-control. (B) The tumor growth in nude mice (six mice per group) inoculated with CH2879 cells treated with pcDNA-MALAT-1 or pcDNA. Each value represents the mean ± SD of triplicate wells. *P<0.05, vs control; **P<0.01, vs control.Abbreviations: MALAT-1, metastasis-associated lung adenocarcinoma transcript 1; SD, standard deviation.

Mentions: To determine the effect of MALAT-1 in vivo, we evaluated its effect in a nude mouse xenograft model of JJ012 cells or CH2879 cells. JJ012 cells were transfected with si-MALAT-1 or si-control and subcutaneously inoculated into the nude mice (six mice per group). Then, the tumor volume was measured. Si-MALAT-1 inhibited tumor growth significantly after inoculation for 28 days (Figure 6A). CH2879 cells transfected with pcDNA-MALAT-1 or pcDNA were subcutaneously inoculated into the nude mice (six mice per group) and the tumor volume was measured. As shown in Figure 6B, the tumor volume was increased in xenograft tumors of mice treated with CH2879 cells containing pcDNA-MALAT-1 compared with the control. Results in vivo further confirmed the promoted effect of MALAT-1 on the tumor growth.


Metastasis-associated lung adenocarcinoma transcript 1 promotes the proliferation of chondrosarcoma cell via activating Notch-1 signaling pathway.

Xu F, Zhang ZQ, Fang YC, Li XL, Sun Y, Xiong CZ, Yan LQ, Wang Q - Onco Targets Ther (2016)

The effect of MALAT-1 on the tumor growth in nude mice.Notes: (A) The tumor growth in nude mice (six mice per group) inoculated with JJ012 cells treated with si-MALAT-1 or si-control. (B) The tumor growth in nude mice (six mice per group) inoculated with CH2879 cells treated with pcDNA-MALAT-1 or pcDNA. Each value represents the mean ± SD of triplicate wells. *P<0.05, vs control; **P<0.01, vs control.Abbreviations: MALAT-1, metastasis-associated lung adenocarcinoma transcript 1; SD, standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835120&req=5

f6-ott-9-2143: The effect of MALAT-1 on the tumor growth in nude mice.Notes: (A) The tumor growth in nude mice (six mice per group) inoculated with JJ012 cells treated with si-MALAT-1 or si-control. (B) The tumor growth in nude mice (six mice per group) inoculated with CH2879 cells treated with pcDNA-MALAT-1 or pcDNA. Each value represents the mean ± SD of triplicate wells. *P<0.05, vs control; **P<0.01, vs control.Abbreviations: MALAT-1, metastasis-associated lung adenocarcinoma transcript 1; SD, standard deviation.
Mentions: To determine the effect of MALAT-1 in vivo, we evaluated its effect in a nude mouse xenograft model of JJ012 cells or CH2879 cells. JJ012 cells were transfected with si-MALAT-1 or si-control and subcutaneously inoculated into the nude mice (six mice per group). Then, the tumor volume was measured. Si-MALAT-1 inhibited tumor growth significantly after inoculation for 28 days (Figure 6A). CH2879 cells transfected with pcDNA-MALAT-1 or pcDNA were subcutaneously inoculated into the nude mice (six mice per group) and the tumor volume was measured. As shown in Figure 6B, the tumor volume was increased in xenograft tumors of mice treated with CH2879 cells containing pcDNA-MALAT-1 compared with the control. Results in vivo further confirmed the promoted effect of MALAT-1 on the tumor growth.

Bottom Line: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is identified to be overexpressed in several cancers.A subcutaneous chondrosarcoma cells xenograft model was used to confirm the effect of MALAT-1 on tumor growth in vivo.Finally, we found that MALAT-1 promoted the tumor growth in a subcutaneous chondrosarcoma cells xenograft model, which confirmed the promoted effect of MALAT-1 on the tumor growth in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, Hongquan Hospital, Yangzhou, Jiangsu Province, People's Republic of China.

ABSTRACT

Background: Metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) is identified to be overexpressed in several cancers. However, the role of MALAT-1 in chondrosarcoma is poorly understood.

Methods: The expression of MALAT-1 and Notch-1 signaling pathway was detected in chondrosarcoma tissues and chondrosarcoma cells by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was performed to examine the cell viability of chondrosarcoma cells transfected with si-MALAT-1 or pcDNA-MALAT-1. Then the expression of Notch-1 signaling pathway was detected when MALAT-1 was upregulated or downregulated in chondrosarcoma cells. A subcutaneous chondrosarcoma cells xenograft model was used to confirm the effect of MALAT-1 on tumor growth in vivo.

Results: We found the increased expression of MALAT-1 and Notch-1 signaling pathway in chondrosarcoma tissue and cells. MALAT-1 promoted the proliferation of chondrosarcoma cells. In addition, MALAT-1 activated the Notch-1 signaling pathway at posttranscriptional level in chondrosarcoma cells. Meanwhile, overexpression of Notch-1 reversed the effect of si-MALAT-1 on the proliferation of chondrosarcoma cells. Finally, we found that MALAT-1 promoted the tumor growth in a subcutaneous chondrosarcoma cells xenograft model, which confirmed the promoted effect of MALAT-1 on the tumor growth in vivo.

Conclusion: Taken together, our study demonstrated that MALAT-1 promoted the proliferation of chondrosarcoma cell via activating Notch-1 signaling pathway.

No MeSH data available.


Related in: MedlinePlus