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Characterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance.

Duan X, Zhang Z, Wang J, Zuo K - PLoS ONE (2016)

Bottom Line: Verticillium pathogens secrete various disease-causing effectors in cotton.Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments.In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, School of Agriculture and Life Sciences, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Verticillium wilt is a disastrous vascular disease in plants caused by Verticillium dahliae. Verticillium pathogens secrete various disease-causing effectors in cotton. This study identified a subtilase gene GbSBT1 from Gossypium babardense and investigated the roles against V. dahliae infection. GbSBT1 gene expression is responsive to V. dahliae defense signals, jasmonic acid, and ethylene treatments. Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments. Silencing GbSBT1 gene expression through virus-induced GbSBT1 gene silencing reduced the tolerance of Pima-90 (resistant genotype), but not facilitated the infection process of V. dahliae in Coker-312 (sensitive genotype). Moreover, the ectopically expressed GbSBT1 gene enhanced the resistance of Arabidopsis to Fusarium oxysporum and V. dahliae infection and activated the expression levels of defense-related genes. Furthermore, pull-down, yeast two-hybrid assay, and BiFC analysis revealed that GbSBT1 interacts with a prohibitin (PHB)-like protein expressed in V. dahliae pathogens during infection. In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

No MeSH data available.


Related in: MedlinePlus

Induced GbSBT1 expression following V. dahliae V991 infection.(A) Expression analysis of the GbSBT1 gene in vegetative tissues (roots, stems, and leaves) via qPCR. The Ubiquitin gene is used as an internal control. At least three biological replicates were performed. (B) Expression analysis of GbSBT1 in roots following V. dahliae strain V991 infection. The comparative CT method is adopted, and the expression is normalized. Each sample was repeated at least thrice. Error bars represent SE. Double asterisks represent a significant expression change of GbSBT1 compared to uninfected condition (P < 0.01) in t-test.
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pone.0153988.g003: Induced GbSBT1 expression following V. dahliae V991 infection.(A) Expression analysis of the GbSBT1 gene in vegetative tissues (roots, stems, and leaves) via qPCR. The Ubiquitin gene is used as an internal control. At least three biological replicates were performed. (B) Expression analysis of GbSBT1 in roots following V. dahliae strain V991 infection. The comparative CT method is adopted, and the expression is normalized. Each sample was repeated at least thrice. Error bars represent SE. Double asterisks represent a significant expression change of GbSBT1 compared to uninfected condition (P < 0.01) in t-test.

Mentions: qPCR was used to analyze the expression pattern of GbSBT1 in various tissues of sea-island cotton. Fig 3A shows that the GbSBT1 gene was ubiquitously expressed in all of the tested tissues. Among the tested tissues, GbSBT1 expressed much higher in root where the V. dahliae enters through the roots more often than other organs.


Characterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance.

Duan X, Zhang Z, Wang J, Zuo K - PLoS ONE (2016)

Induced GbSBT1 expression following V. dahliae V991 infection.(A) Expression analysis of the GbSBT1 gene in vegetative tissues (roots, stems, and leaves) via qPCR. The Ubiquitin gene is used as an internal control. At least three biological replicates were performed. (B) Expression analysis of GbSBT1 in roots following V. dahliae strain V991 infection. The comparative CT method is adopted, and the expression is normalized. Each sample was repeated at least thrice. Error bars represent SE. Double asterisks represent a significant expression change of GbSBT1 compared to uninfected condition (P < 0.01) in t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835097&req=5

pone.0153988.g003: Induced GbSBT1 expression following V. dahliae V991 infection.(A) Expression analysis of the GbSBT1 gene in vegetative tissues (roots, stems, and leaves) via qPCR. The Ubiquitin gene is used as an internal control. At least three biological replicates were performed. (B) Expression analysis of GbSBT1 in roots following V. dahliae strain V991 infection. The comparative CT method is adopted, and the expression is normalized. Each sample was repeated at least thrice. Error bars represent SE. Double asterisks represent a significant expression change of GbSBT1 compared to uninfected condition (P < 0.01) in t-test.
Mentions: qPCR was used to analyze the expression pattern of GbSBT1 in various tissues of sea-island cotton. Fig 3A shows that the GbSBT1 gene was ubiquitously expressed in all of the tested tissues. Among the tested tissues, GbSBT1 expressed much higher in root where the V. dahliae enters through the roots more often than other organs.

Bottom Line: Verticillium pathogens secrete various disease-causing effectors in cotton.Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments.In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, School of Agriculture and Life Sciences, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Verticillium wilt is a disastrous vascular disease in plants caused by Verticillium dahliae. Verticillium pathogens secrete various disease-causing effectors in cotton. This study identified a subtilase gene GbSBT1 from Gossypium babardense and investigated the roles against V. dahliae infection. GbSBT1 gene expression is responsive to V. dahliae defense signals, jasmonic acid, and ethylene treatments. Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments. Silencing GbSBT1 gene expression through virus-induced GbSBT1 gene silencing reduced the tolerance of Pima-90 (resistant genotype), but not facilitated the infection process of V. dahliae in Coker-312 (sensitive genotype). Moreover, the ectopically expressed GbSBT1 gene enhanced the resistance of Arabidopsis to Fusarium oxysporum and V. dahliae infection and activated the expression levels of defense-related genes. Furthermore, pull-down, yeast two-hybrid assay, and BiFC analysis revealed that GbSBT1 interacts with a prohibitin (PHB)-like protein expressed in V. dahliae pathogens during infection. In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

No MeSH data available.


Related in: MedlinePlus