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Characterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance.

Duan X, Zhang Z, Wang J, Zuo K - PLoS ONE (2016)

Bottom Line: Verticillium pathogens secrete various disease-causing effectors in cotton.Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments.In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, School of Agriculture and Life Sciences, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Verticillium wilt is a disastrous vascular disease in plants caused by Verticillium dahliae. Verticillium pathogens secrete various disease-causing effectors in cotton. This study identified a subtilase gene GbSBT1 from Gossypium babardense and investigated the roles against V. dahliae infection. GbSBT1 gene expression is responsive to V. dahliae defense signals, jasmonic acid, and ethylene treatments. Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments. Silencing GbSBT1 gene expression through virus-induced GbSBT1 gene silencing reduced the tolerance of Pima-90 (resistant genotype), but not facilitated the infection process of V. dahliae in Coker-312 (sensitive genotype). Moreover, the ectopically expressed GbSBT1 gene enhanced the resistance of Arabidopsis to Fusarium oxysporum and V. dahliae infection and activated the expression levels of defense-related genes. Furthermore, pull-down, yeast two-hybrid assay, and BiFC analysis revealed that GbSBT1 interacts with a prohibitin (PHB)-like protein expressed in V. dahliae pathogens during infection. In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

No MeSH data available.


Related in: MedlinePlus

Multiple sequence alignment analysis of GbSBT1 and other plant subtilases.GbSBT1 (KT336228, Gossypium barbadense), subtilase-like (XP_007017870, Theobroma cacao), SBT5.2 (NP_564107, Arabidopsis thaliana), SBT-like (AAM65424, A. thaliana). SP: signal peptide; Inhibitor I9: serine protease inhibitor domain; black triangles indicate conserved active sites; blank triangles indicate catalytic triads of serine protease; and blank box A indicates PA/protease or protease-like domain interface.
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pone.0153988.g001: Multiple sequence alignment analysis of GbSBT1 and other plant subtilases.GbSBT1 (KT336228, Gossypium barbadense), subtilase-like (XP_007017870, Theobroma cacao), SBT5.2 (NP_564107, Arabidopsis thaliana), SBT-like (AAM65424, A. thaliana). SP: signal peptide; Inhibitor I9: serine protease inhibitor domain; black triangles indicate conserved active sites; blank triangles indicate catalytic triads of serine protease; and blank box A indicates PA/protease or protease-like domain interface.

Mentions: The GbSBT1 gene encodes a protein containing 769 aa, corresponding to a molecular mass of 81.78 kDa and a pI of 6.34. The GbSBT1 protein contains a putative signal peptide, an inhibitor domain, five conserved active sites (Ser-544, Asp-145, and His-223), and two catalytic triads of serine protease (Fig 1). A neighbor-joining phylogenetic tree of 19 subtilases indicated that that GbSBT1 is closely related to AtSBT5.2 (Fig 2). In Arabidopsis, AtSBT5.2 exhibits oxido-reductase activity and responds to an external biotic stimulus [10], indicating that the GbSBT1 gene may function in biotic stress response.


Characterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance.

Duan X, Zhang Z, Wang J, Zuo K - PLoS ONE (2016)

Multiple sequence alignment analysis of GbSBT1 and other plant subtilases.GbSBT1 (KT336228, Gossypium barbadense), subtilase-like (XP_007017870, Theobroma cacao), SBT5.2 (NP_564107, Arabidopsis thaliana), SBT-like (AAM65424, A. thaliana). SP: signal peptide; Inhibitor I9: serine protease inhibitor domain; black triangles indicate conserved active sites; blank triangles indicate catalytic triads of serine protease; and blank box A indicates PA/protease or protease-like domain interface.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835097&req=5

pone.0153988.g001: Multiple sequence alignment analysis of GbSBT1 and other plant subtilases.GbSBT1 (KT336228, Gossypium barbadense), subtilase-like (XP_007017870, Theobroma cacao), SBT5.2 (NP_564107, Arabidopsis thaliana), SBT-like (AAM65424, A. thaliana). SP: signal peptide; Inhibitor I9: serine protease inhibitor domain; black triangles indicate conserved active sites; blank triangles indicate catalytic triads of serine protease; and blank box A indicates PA/protease or protease-like domain interface.
Mentions: The GbSBT1 gene encodes a protein containing 769 aa, corresponding to a molecular mass of 81.78 kDa and a pI of 6.34. The GbSBT1 protein contains a putative signal peptide, an inhibitor domain, five conserved active sites (Ser-544, Asp-145, and His-223), and two catalytic triads of serine protease (Fig 1). A neighbor-joining phylogenetic tree of 19 subtilases indicated that that GbSBT1 is closely related to AtSBT5.2 (Fig 2). In Arabidopsis, AtSBT5.2 exhibits oxido-reductase activity and responds to an external biotic stimulus [10], indicating that the GbSBT1 gene may function in biotic stress response.

Bottom Line: Verticillium pathogens secrete various disease-causing effectors in cotton.Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments.In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

View Article: PubMed Central - PubMed

Affiliation: Plant Biotechnology Research Center, School of Agriculture and Life Sciences, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT
Verticillium wilt is a disastrous vascular disease in plants caused by Verticillium dahliae. Verticillium pathogens secrete various disease-causing effectors in cotton. This study identified a subtilase gene GbSBT1 from Gossypium babardense and investigated the roles against V. dahliae infection. GbSBT1 gene expression is responsive to V. dahliae defense signals, jasmonic acid, and ethylene treatments. Moreover, the GbSBT1 protein is mainly localized in the cell membrane and moves into the cytoplasm following jasmonic acid and ethylene treatments. Silencing GbSBT1 gene expression through virus-induced GbSBT1 gene silencing reduced the tolerance of Pima-90 (resistant genotype), but not facilitated the infection process of V. dahliae in Coker-312 (sensitive genotype). Moreover, the ectopically expressed GbSBT1 gene enhanced the resistance of Arabidopsis to Fusarium oxysporum and V. dahliae infection and activated the expression levels of defense-related genes. Furthermore, pull-down, yeast two-hybrid assay, and BiFC analysis revealed that GbSBT1 interacts with a prohibitin (PHB)-like protein expressed in V. dahliae pathogens during infection. In summary, GbSBT1 recognizes the effector PHB protein secreted from V. dahliae and is involved in Verticillium-induced resistance in cotton.

No MeSH data available.


Related in: MedlinePlus