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Daboxin P, a Major Phospholipase A2 Enzyme from the Indian Daboia russelii russelii Venom Targets Factor X and Factor Xa for Its Anticoagulant Activity.

Sharma M, Iyer JK, Shih N, Majumder M, Mattaparthi VS, Mukhopadhyay R, Doley R - PLoS ONE (2016)

Bottom Line: It exhibits strong anticoagulant and phospholipase A2 activity but is devoid of any cytotoxic effect on the tested normal or cancerous cell lines.It is found to be stable at acidic (pH 3.0) and neutral pH (pH 7.0) and has a Tm value of 71.59 ± 0.46°C.Molecular docking analysis reveals the interaction of the Ca+2 binding loop; helix C; anticoagulant region and C-terminal region of daboxin P with the heavy chain of factor Xa.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur-784028, Assam, India.

ABSTRACT
In the present study a major protein has been purified from the venom of Indian Daboia russelii russelii using gel filtration, ion exchange and Rp-HPLC techniques. The purified protein, named daboxin P accounts for ~24% of the total protein of the crude venom and has a molecular mass of 13.597 kDa. It exhibits strong anticoagulant and phospholipase A2 activity but is devoid of any cytotoxic effect on the tested normal or cancerous cell lines. Its primary structure was deduced by N-terminal sequencing and chemical cleavage using Edman degradation and tandem mass spectrometry. It is composed of 121 amino acids with 14 cysteine residues and catalytically active His48 -Asp49 pair. The secondary structure of daboxin P constitutes 42.73% of α-helix and 12.36% of β-sheet. It is found to be stable at acidic (pH 3.0) and neutral pH (pH 7.0) and has a Tm value of 71.59 ± 0.46°C. Daboxin P exhibits anticoagulant effect under in-vitro and in-vivo conditions. It does not inhibit the catalytic activity of the serine proteases but inhibits the activation of factor X to factor Xa by the tenase complexes both in the presence and absence of phospholipids. It also inhibits the tenase complexes when active site residue (His48) was alkylated suggesting its non-enzymatic mode of anticoagulant activity. Moreover, it also inhibits prothrombinase complex when pre-incubated with factor Xa prior to factor Va addition. Fluorescence emission spectroscopy and affinity chromatography suggest the probable interaction of daboxin P with factor X and factor Xa. Molecular docking analysis reveals the interaction of the Ca+2 binding loop; helix C; anticoagulant region and C-terminal region of daboxin P with the heavy chain of factor Xa. This is the first report of a phospholipase A2 enzyme from Indian viper venom which targets both factor X and factor Xa for its anticoagulant activity.

No MeSH data available.


Related in: MedlinePlus

Effect of daboxin P on the time to occlusion (TTO) in FeCl3 induced carotid artery thrombosis in mice.C57BL/6 male mice anesthetized with ketamine (75 mg/kg) and medetomidine (1 mg/kg) (i.p) were injected (i.p.) with daboxin P (10 mg/kg) in tail vein. Saline treated mice were considered as negative control. Each data-point represents the time-to-occlusion (TTO) of a single mouse. Maximum experimental time was considered for 60 min after FeCl3 induction.
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pone.0153770.g008: Effect of daboxin P on the time to occlusion (TTO) in FeCl3 induced carotid artery thrombosis in mice.C57BL/6 male mice anesthetized with ketamine (75 mg/kg) and medetomidine (1 mg/kg) (i.p) were injected (i.p.) with daboxin P (10 mg/kg) in tail vein. Saline treated mice were considered as negative control. Each data-point represents the time-to-occlusion (TTO) of a single mouse. Maximum experimental time was considered for 60 min after FeCl3 induction.

Mentions: Daboxin P exhibited anticoagulant effect in the carotid artery of mouse treated with FeCl3. Mice administered with saline had a time-to-occlusion within 10 min. However, daboxin P administration resulted in ~4-fold increase (35.18 ± 21.58 min) in the time-to-occlusion in comparison to the saline treated mice (8.29 ± 2.61 min) (Fig 8). This result was in accordance with the in-vitro anticoagulant assays on human plasma and highlighted the anticoagulant property of daboxin P under in-vivo condition.


Daboxin P, a Major Phospholipase A2 Enzyme from the Indian Daboia russelii russelii Venom Targets Factor X and Factor Xa for Its Anticoagulant Activity.

Sharma M, Iyer JK, Shih N, Majumder M, Mattaparthi VS, Mukhopadhyay R, Doley R - PLoS ONE (2016)

Effect of daboxin P on the time to occlusion (TTO) in FeCl3 induced carotid artery thrombosis in mice.C57BL/6 male mice anesthetized with ketamine (75 mg/kg) and medetomidine (1 mg/kg) (i.p) were injected (i.p.) with daboxin P (10 mg/kg) in tail vein. Saline treated mice were considered as negative control. Each data-point represents the time-to-occlusion (TTO) of a single mouse. Maximum experimental time was considered for 60 min after FeCl3 induction.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835082&req=5

pone.0153770.g008: Effect of daboxin P on the time to occlusion (TTO) in FeCl3 induced carotid artery thrombosis in mice.C57BL/6 male mice anesthetized with ketamine (75 mg/kg) and medetomidine (1 mg/kg) (i.p) were injected (i.p.) with daboxin P (10 mg/kg) in tail vein. Saline treated mice were considered as negative control. Each data-point represents the time-to-occlusion (TTO) of a single mouse. Maximum experimental time was considered for 60 min after FeCl3 induction.
Mentions: Daboxin P exhibited anticoagulant effect in the carotid artery of mouse treated with FeCl3. Mice administered with saline had a time-to-occlusion within 10 min. However, daboxin P administration resulted in ~4-fold increase (35.18 ± 21.58 min) in the time-to-occlusion in comparison to the saline treated mice (8.29 ± 2.61 min) (Fig 8). This result was in accordance with the in-vitro anticoagulant assays on human plasma and highlighted the anticoagulant property of daboxin P under in-vivo condition.

Bottom Line: It exhibits strong anticoagulant and phospholipase A2 activity but is devoid of any cytotoxic effect on the tested normal or cancerous cell lines.It is found to be stable at acidic (pH 3.0) and neutral pH (pH 7.0) and has a Tm value of 71.59 ± 0.46°C.Molecular docking analysis reveals the interaction of the Ca+2 binding loop; helix C; anticoagulant region and C-terminal region of daboxin P with the heavy chain of factor Xa.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur-784028, Assam, India.

ABSTRACT
In the present study a major protein has been purified from the venom of Indian Daboia russelii russelii using gel filtration, ion exchange and Rp-HPLC techniques. The purified protein, named daboxin P accounts for ~24% of the total protein of the crude venom and has a molecular mass of 13.597 kDa. It exhibits strong anticoagulant and phospholipase A2 activity but is devoid of any cytotoxic effect on the tested normal or cancerous cell lines. Its primary structure was deduced by N-terminal sequencing and chemical cleavage using Edman degradation and tandem mass spectrometry. It is composed of 121 amino acids with 14 cysteine residues and catalytically active His48 -Asp49 pair. The secondary structure of daboxin P constitutes 42.73% of α-helix and 12.36% of β-sheet. It is found to be stable at acidic (pH 3.0) and neutral pH (pH 7.0) and has a Tm value of 71.59 ± 0.46°C. Daboxin P exhibits anticoagulant effect under in-vitro and in-vivo conditions. It does not inhibit the catalytic activity of the serine proteases but inhibits the activation of factor X to factor Xa by the tenase complexes both in the presence and absence of phospholipids. It also inhibits the tenase complexes when active site residue (His48) was alkylated suggesting its non-enzymatic mode of anticoagulant activity. Moreover, it also inhibits prothrombinase complex when pre-incubated with factor Xa prior to factor Va addition. Fluorescence emission spectroscopy and affinity chromatography suggest the probable interaction of daboxin P with factor X and factor Xa. Molecular docking analysis reveals the interaction of the Ca+2 binding loop; helix C; anticoagulant region and C-terminal region of daboxin P with the heavy chain of factor Xa. This is the first report of a phospholipase A2 enzyme from Indian viper venom which targets both factor X and factor Xa for its anticoagulant activity.

No MeSH data available.


Related in: MedlinePlus