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Geographical and Ethnic Distributions of the MTHFR C677T, A1298C and MTRR A66G Gene Polymorphisms in Chinese Populations: A Meta-Analysis.

Wang X, Fu J, Li Q, Zeng D - PLoS ONE (2016)

Bottom Line: The frequency estimates were stratified by geography, ethnicity and sex.Sixty-six studies were identified with a total of 92277 study participants.Our meta-analysis strongly indicates significant geographical and ethnic variations in the frequencies of the C677T, A1298C, and A66G gene polymorphisms in the folate metabolism pathway among Chinese populations.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, Liuzhou Municipal Maternity and Child Healthcare Hospital, Liuzhou, Guangxi, China.

ABSTRACT

Background: The geographical and ethnic distributions of the polymorphic methylenetetrahydrofolate reductase (MTHFR) mutations (C677T and A1298C) and methionine synthase reductase (MTRR) mutation (A66G) remain heterogeneous in China. The goal of this study was to estimate the pooled frequencies of the alleles and associated genotypes of these gene polymorphisms among healthy populations in Mainland China.

Objective and methods: We systematically reviewed published epidemiological studies on the distributions of 3 genetic variants in Chinese healthy populations living in Mainland China through a meta-analysis. The relevant electronic databases were searched. All of the raw data of the eligible citations were extracted. The frequency estimates were stratified by geography, ethnicity and sex.

Results: Sixty-six studies were identified with a total of 92277 study participants. The meta-analysis revealed that the frequencies of the MTHFR C677T, A1298C, and MTRR A66G gene polymorphisms varied significantly between different ethnic groups and along geographical gradients. The frequencies of the 677T allele and 677TT genotype increased along the southern-central-northern direction across Mainland China (all Pvalues≤0.001). The frequencies of the 1298C, 1298CC, 66G and 66GG genotypes decreased along the south-central-north direction across the country (all Pvalues≤0.001).

Conclusions: Our meta-analysis strongly indicates significant geographical and ethnic variations in the frequencies of the C677T, A1298C, and A66G gene polymorphisms in the folate metabolism pathway among Chinese populations.

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Flow chart of the study selection process.
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pone.0152414.g001: Flow chart of the study selection process.

Mentions: In total, 495 articles were identified, of which 471 potentially relevant citations were included for further evaluation. Eventually, 68 articles (66 on the C677T, 51 on the A1298C, and 43 on the A66G polymorphisms) with a total of 92277 participants met the inclusion criteria [18–85] (Fig 1). The main characteristics of the studies on the MTHFR C677T and A1298C and MTRR A66G polymorphisms are listed in Tables 1–3, respectively. In the majority of the studies, buccal cells were obtained and tested with real-time polymerase chain reaction (RT-PCR); otherwise, blood samples were tested with restriction fragment length polymorphism (RFLP) analysis. The genotype frequencies indicated that all of the polymorphisms were in HWE in all of the samples.


Geographical and Ethnic Distributions of the MTHFR C677T, A1298C and MTRR A66G Gene Polymorphisms in Chinese Populations: A Meta-Analysis.

Wang X, Fu J, Li Q, Zeng D - PLoS ONE (2016)

Flow chart of the study selection process.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835080&req=5

pone.0152414.g001: Flow chart of the study selection process.
Mentions: In total, 495 articles were identified, of which 471 potentially relevant citations were included for further evaluation. Eventually, 68 articles (66 on the C677T, 51 on the A1298C, and 43 on the A66G polymorphisms) with a total of 92277 participants met the inclusion criteria [18–85] (Fig 1). The main characteristics of the studies on the MTHFR C677T and A1298C and MTRR A66G polymorphisms are listed in Tables 1–3, respectively. In the majority of the studies, buccal cells were obtained and tested with real-time polymerase chain reaction (RT-PCR); otherwise, blood samples were tested with restriction fragment length polymorphism (RFLP) analysis. The genotype frequencies indicated that all of the polymorphisms were in HWE in all of the samples.

Bottom Line: The frequency estimates were stratified by geography, ethnicity and sex.Sixty-six studies were identified with a total of 92277 study participants.Our meta-analysis strongly indicates significant geographical and ethnic variations in the frequencies of the C677T, A1298C, and A66G gene polymorphisms in the folate metabolism pathway among Chinese populations.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, Liuzhou Municipal Maternity and Child Healthcare Hospital, Liuzhou, Guangxi, China.

ABSTRACT

Background: The geographical and ethnic distributions of the polymorphic methylenetetrahydrofolate reductase (MTHFR) mutations (C677T and A1298C) and methionine synthase reductase (MTRR) mutation (A66G) remain heterogeneous in China. The goal of this study was to estimate the pooled frequencies of the alleles and associated genotypes of these gene polymorphisms among healthy populations in Mainland China.

Objective and methods: We systematically reviewed published epidemiological studies on the distributions of 3 genetic variants in Chinese healthy populations living in Mainland China through a meta-analysis. The relevant electronic databases were searched. All of the raw data of the eligible citations were extracted. The frequency estimates were stratified by geography, ethnicity and sex.

Results: Sixty-six studies were identified with a total of 92277 study participants. The meta-analysis revealed that the frequencies of the MTHFR C677T, A1298C, and MTRR A66G gene polymorphisms varied significantly between different ethnic groups and along geographical gradients. The frequencies of the 677T allele and 677TT genotype increased along the southern-central-northern direction across Mainland China (all Pvalues≤0.001). The frequencies of the 1298C, 1298CC, 66G and 66GG genotypes decreased along the south-central-north direction across the country (all Pvalues≤0.001).

Conclusions: Our meta-analysis strongly indicates significant geographical and ethnic variations in the frequencies of the C677T, A1298C, and A66G gene polymorphisms in the folate metabolism pathway among Chinese populations.

Show MeSH
Related in: MedlinePlus