Limits...
Electric Cell-Substrate Impedance Sensing (ECIS) with Microelectrode Arrays for Investigation of Cancer Cell-Fibroblasts Interaction.

Tran TB, Baek C, Min J - PLoS ONE (2016)

Bottom Line: In brief, a co-culture device consisting of 2 individual fluidic chambers in parallel, which were separated by a 100 μm fence was utilized for cell patterning.Microelectrodes arrays were installed within each chamber including electrodes at various distances away from the confrontation line for the electrochemical impedimetric sensing assessment of cell-to-cell influence.After the fence was removed and cell-to-cell contact occurred, by evaluating the impedance signal responses representing cell condition and behavior, both direct and indirect cell-to-cell interactions through conditioned media were investigated.

View Article: PubMed Central - PubMed

Affiliation: School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea.

ABSTRACT
The tumor microenvironment, including stromal cells, surrounding blood vessels and extracellular matrix components, has been defined as a crucial factor that influences the proliferation, drug-resistance, invasion and metastasis of malignant epithelial cells. Among other factors, the communications and interaction between cancer cells and stromal cells have been reported to play pivotal roles in cancer promotion and progression. To investigate these relationships, an on-chip co-culture model was developed to study the cellular interaction between A549-human lung carcinoma cells and MRC-5-human lung epithelial cells in both normal proliferation and treatment conditions. In brief, a co-culture device consisting of 2 individual fluidic chambers in parallel, which were separated by a 100 μm fence was utilized for cell patterning. Microelectrodes arrays were installed within each chamber including electrodes at various distances away from the confrontation line for the electrochemical impedimetric sensing assessment of cell-to-cell influence. After the fence was removed and cell-to-cell contact occurred, by evaluating the impedance signal responses representing cell condition and behavior, both direct and indirect cell-to-cell interactions through conditioned media were investigated. The impact of specific distances that lead to different influences of fibroblast cells on cancer cells in the co-culture environment was also defined.

No MeSH data available.


Related in: MedlinePlus

Histogram results of curcumin treatment at 30μM by FACS assay.Different percentages of apoptotic cells were obtained in the A549 tumor cells and MRC-5 fibroblasts monocultures and in the mixed culture of these 2 cell lines. [A color figure can be viewed in the online issue].
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835071&req=5

pone.0153813.g003: Histogram results of curcumin treatment at 30μM by FACS assay.Different percentages of apoptotic cells were obtained in the A549 tumor cells and MRC-5 fibroblasts monocultures and in the mixed culture of these 2 cell lines. [A color figure can be viewed in the online issue].

Mentions: As shown in Fig 3, curcumin treatment in the monocultures induced apoptosis in both A549 cells and MRC-5 cells at 9.87% and 19.05% apoptotic cells, respectively. This ratio was less significant than our MTS results as well as the results of other previous studies [20]. These differences were explained by the lack of dead cells, which cannot be discriminated by FACS with only PS staining.


Electric Cell-Substrate Impedance Sensing (ECIS) with Microelectrode Arrays for Investigation of Cancer Cell-Fibroblasts Interaction.

Tran TB, Baek C, Min J - PLoS ONE (2016)

Histogram results of curcumin treatment at 30μM by FACS assay.Different percentages of apoptotic cells were obtained in the A549 tumor cells and MRC-5 fibroblasts monocultures and in the mixed culture of these 2 cell lines. [A color figure can be viewed in the online issue].
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835071&req=5

pone.0153813.g003: Histogram results of curcumin treatment at 30μM by FACS assay.Different percentages of apoptotic cells were obtained in the A549 tumor cells and MRC-5 fibroblasts monocultures and in the mixed culture of these 2 cell lines. [A color figure can be viewed in the online issue].
Mentions: As shown in Fig 3, curcumin treatment in the monocultures induced apoptosis in both A549 cells and MRC-5 cells at 9.87% and 19.05% apoptotic cells, respectively. This ratio was less significant than our MTS results as well as the results of other previous studies [20]. These differences were explained by the lack of dead cells, which cannot be discriminated by FACS with only PS staining.

Bottom Line: In brief, a co-culture device consisting of 2 individual fluidic chambers in parallel, which were separated by a 100 μm fence was utilized for cell patterning.Microelectrodes arrays were installed within each chamber including electrodes at various distances away from the confrontation line for the electrochemical impedimetric sensing assessment of cell-to-cell influence.After the fence was removed and cell-to-cell contact occurred, by evaluating the impedance signal responses representing cell condition and behavior, both direct and indirect cell-to-cell interactions through conditioned media were investigated.

View Article: PubMed Central - PubMed

Affiliation: School of Integrative Engineering, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul, Republic of Korea.

ABSTRACT
The tumor microenvironment, including stromal cells, surrounding blood vessels and extracellular matrix components, has been defined as a crucial factor that influences the proliferation, drug-resistance, invasion and metastasis of malignant epithelial cells. Among other factors, the communications and interaction between cancer cells and stromal cells have been reported to play pivotal roles in cancer promotion and progression. To investigate these relationships, an on-chip co-culture model was developed to study the cellular interaction between A549-human lung carcinoma cells and MRC-5-human lung epithelial cells in both normal proliferation and treatment conditions. In brief, a co-culture device consisting of 2 individual fluidic chambers in parallel, which were separated by a 100 μm fence was utilized for cell patterning. Microelectrodes arrays were installed within each chamber including electrodes at various distances away from the confrontation line for the electrochemical impedimetric sensing assessment of cell-to-cell influence. After the fence was removed and cell-to-cell contact occurred, by evaluating the impedance signal responses representing cell condition and behavior, both direct and indirect cell-to-cell interactions through conditioned media were investigated. The impact of specific distances that lead to different influences of fibroblast cells on cancer cells in the co-culture environment was also defined.

No MeSH data available.


Related in: MedlinePlus