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Interleukin-25 Mediated Induction of Angiogenin-4 Is Interleukin-13 Dependent.

Noor Z, Burgess SL, Watanabe K, Petri WA - PLoS ONE (2016)

Bottom Line: The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues.Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4.IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.

ABSTRACT
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

No MeSH data available.


rIL-25 induced angiogenin-4 production is not significantly influenced by IL-17 or IL-22 blockade.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 each day for total 7 doses and control mice received PBS. rIL-25 treated mice received 200μg anti IL-17 antibody (inversed triangle) or 200μg anti IL-22 antibody (open square) or 200μg anti-IL-13 antibody (open circle), or isotype control on day 3, on day 5 and on day 7. Angiogenin-4 relative expression was measured from cecal tissue.
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pone.0153572.g006: rIL-25 induced angiogenin-4 production is not significantly influenced by IL-17 or IL-22 blockade.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 each day for total 7 doses and control mice received PBS. rIL-25 treated mice received 200μg anti IL-17 antibody (inversed triangle) or 200μg anti IL-22 antibody (open square) or 200μg anti-IL-13 antibody (open circle), or isotype control on day 3, on day 5 and on day 7. Angiogenin-4 relative expression was measured from cecal tissue.

Mentions: IL-17 and IL-22 are both known to be potent inducers of angiogenin-4 from Paneth cells during infection. We therefore tested the requirement of IL-22 or IL-17 in IL-25 mediated angiogenin-4 production. We depleted IL-22 or IL-17 by monoclonal antibodies in rIL-25 treated mice. We found that angiogenin-4 expression was partially decreased by the depletion of either IL-22 or IL-17 in rIL-25 treated mice, whereas IL-13 neutralization completely abrogated IL-25 induced angiogenin-4 expression (Fig 6). From these results, we concluded IL-25 induced angiogenin-4 expression largely depends on downstream IL-13, rather than IL-22 or IL-17.


Interleukin-25 Mediated Induction of Angiogenin-4 Is Interleukin-13 Dependent.

Noor Z, Burgess SL, Watanabe K, Petri WA - PLoS ONE (2016)

rIL-25 induced angiogenin-4 production is not significantly influenced by IL-17 or IL-22 blockade.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 each day for total 7 doses and control mice received PBS. rIL-25 treated mice received 200μg anti IL-17 antibody (inversed triangle) or 200μg anti IL-22 antibody (open square) or 200μg anti-IL-13 antibody (open circle), or isotype control on day 3, on day 5 and on day 7. Angiogenin-4 relative expression was measured from cecal tissue.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835063&req=5

pone.0153572.g006: rIL-25 induced angiogenin-4 production is not significantly influenced by IL-17 or IL-22 blockade.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 each day for total 7 doses and control mice received PBS. rIL-25 treated mice received 200μg anti IL-17 antibody (inversed triangle) or 200μg anti IL-22 antibody (open square) or 200μg anti-IL-13 antibody (open circle), or isotype control on day 3, on day 5 and on day 7. Angiogenin-4 relative expression was measured from cecal tissue.
Mentions: IL-17 and IL-22 are both known to be potent inducers of angiogenin-4 from Paneth cells during infection. We therefore tested the requirement of IL-22 or IL-17 in IL-25 mediated angiogenin-4 production. We depleted IL-22 or IL-17 by monoclonal antibodies in rIL-25 treated mice. We found that angiogenin-4 expression was partially decreased by the depletion of either IL-22 or IL-17 in rIL-25 treated mice, whereas IL-13 neutralization completely abrogated IL-25 induced angiogenin-4 expression (Fig 6). From these results, we concluded IL-25 induced angiogenin-4 expression largely depends on downstream IL-13, rather than IL-22 or IL-17.

Bottom Line: The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues.Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4.IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.

ABSTRACT
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

No MeSH data available.