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Interleukin-25 Mediated Induction of Angiogenin-4 Is Interleukin-13 Dependent.

Noor Z, Burgess SL, Watanabe K, Petri WA - PLoS ONE (2016)

Bottom Line: The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues.Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4.IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.

ABSTRACT
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

No MeSH data available.


rIL-25 administration increases angiogenin-4 expression in a dose dependent manner.CBA/J mice were treated with 0.5 micrograms of rIL-25 each day for a total 4 doses (triangle, n = 7) or 8 doses (inverse triangle, n = 5) and control mice received 4 or 8 doses of PBS (open circle, n = 7 for 4 doses and n = 5 for 8 doses). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin.
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pone.0153572.g002: rIL-25 administration increases angiogenin-4 expression in a dose dependent manner.CBA/J mice were treated with 0.5 micrograms of rIL-25 each day for a total 4 doses (triangle, n = 7) or 8 doses (inverse triangle, n = 5) and control mice received 4 or 8 doses of PBS (open circle, n = 7 for 4 doses and n = 5 for 8 doses). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin.

Mentions: In order to test if IL-25 induces angiogenin-4 production, we treated CBA/J mice with recombinant IL-25 (rIL-25), and then we measured in mouse ceca the mRNA expression level of angiogenin-4 by qPCR. We found that angiogenin-4 expression was more than 100 fold higher in rIL-25 treated mice than in PBS treated mice (Fig 1A). We confirmed this pattern of expression by performing immunohistochemistry for angiogenin-4 in cecal tissue. Angiogenin-4 protein expression was highly upregulated in rIL-25 treated mice with expression observed in intestinal epithelial cells in the crypts and villi (Fig 1B–1D). We then tested for a dose dependent induction of angiogenin-4 by rIL-25. We found that 4 vs 8 doses of rIL-25 induced a 7-fold and 218-fold increase in angiogenin-4 compared to the PBS control (Fig 2). We concluded that IL-25 induced angiogenin-4 expression in the cecal intestinal epithelium.


Interleukin-25 Mediated Induction of Angiogenin-4 Is Interleukin-13 Dependent.

Noor Z, Burgess SL, Watanabe K, Petri WA - PLoS ONE (2016)

rIL-25 administration increases angiogenin-4 expression in a dose dependent manner.CBA/J mice were treated with 0.5 micrograms of rIL-25 each day for a total 4 doses (triangle, n = 7) or 8 doses (inverse triangle, n = 5) and control mice received 4 or 8 doses of PBS (open circle, n = 7 for 4 doses and n = 5 for 8 doses). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835063&req=5

pone.0153572.g002: rIL-25 administration increases angiogenin-4 expression in a dose dependent manner.CBA/J mice were treated with 0.5 micrograms of rIL-25 each day for a total 4 doses (triangle, n = 7) or 8 doses (inverse triangle, n = 5) and control mice received 4 or 8 doses of PBS (open circle, n = 7 for 4 doses and n = 5 for 8 doses). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin.
Mentions: In order to test if IL-25 induces angiogenin-4 production, we treated CBA/J mice with recombinant IL-25 (rIL-25), and then we measured in mouse ceca the mRNA expression level of angiogenin-4 by qPCR. We found that angiogenin-4 expression was more than 100 fold higher in rIL-25 treated mice than in PBS treated mice (Fig 1A). We confirmed this pattern of expression by performing immunohistochemistry for angiogenin-4 in cecal tissue. Angiogenin-4 protein expression was highly upregulated in rIL-25 treated mice with expression observed in intestinal epithelial cells in the crypts and villi (Fig 1B–1D). We then tested for a dose dependent induction of angiogenin-4 by rIL-25. We found that 4 vs 8 doses of rIL-25 induced a 7-fold and 218-fold increase in angiogenin-4 compared to the PBS control (Fig 2). We concluded that IL-25 induced angiogenin-4 expression in the cecal intestinal epithelium.

Bottom Line: The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues.Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4.IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.

ABSTRACT
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

No MeSH data available.