Limits...
Interleukin-25 Mediated Induction of Angiogenin-4 Is Interleukin-13 Dependent.

Noor Z, Burgess SL, Watanabe K, Petri WA - PLoS ONE (2016)

Bottom Line: The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues.Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4.IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.

ABSTRACT
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

No MeSH data available.


rIL-25 administration induces angiogenin-4 expression.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 (triangle, n = 11) each day for a total of 10 doses over 10 days. Control mice received PBS (open circle, n = 11). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin (A). Histological scoring (1 to 5; low to high) for Angiogenin-4 in cecum from mice treated with PBS or rIL-25 (B). Representative IHC staining for angiogenin-4 in samples of cecum tissue from PBS treated (C) or rIL-25 treated mice (D).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835063&req=5

pone.0153572.g001: rIL-25 administration induces angiogenin-4 expression.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 (triangle, n = 11) each day for a total of 10 doses over 10 days. Control mice received PBS (open circle, n = 11). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin (A). Histological scoring (1 to 5; low to high) for Angiogenin-4 in cecum from mice treated with PBS or rIL-25 (B). Representative IHC staining for angiogenin-4 in samples of cecum tissue from PBS treated (C) or rIL-25 treated mice (D).

Mentions: In order to test if IL-25 induces angiogenin-4 production, we treated CBA/J mice with recombinant IL-25 (rIL-25), and then we measured in mouse ceca the mRNA expression level of angiogenin-4 by qPCR. We found that angiogenin-4 expression was more than 100 fold higher in rIL-25 treated mice than in PBS treated mice (Fig 1A). We confirmed this pattern of expression by performing immunohistochemistry for angiogenin-4 in cecal tissue. Angiogenin-4 protein expression was highly upregulated in rIL-25 treated mice with expression observed in intestinal epithelial cells in the crypts and villi (Fig 1B–1D). We then tested for a dose dependent induction of angiogenin-4 by rIL-25. We found that 4 vs 8 doses of rIL-25 induced a 7-fold and 218-fold increase in angiogenin-4 compared to the PBS control (Fig 2). We concluded that IL-25 induced angiogenin-4 expression in the cecal intestinal epithelium.


Interleukin-25 Mediated Induction of Angiogenin-4 Is Interleukin-13 Dependent.

Noor Z, Burgess SL, Watanabe K, Petri WA - PLoS ONE (2016)

rIL-25 administration induces angiogenin-4 expression.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 (triangle, n = 11) each day for a total of 10 doses over 10 days. Control mice received PBS (open circle, n = 11). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin (A). Histological scoring (1 to 5; low to high) for Angiogenin-4 in cecum from mice treated with PBS or rIL-25 (B). Representative IHC staining for angiogenin-4 in samples of cecum tissue from PBS treated (C) or rIL-25 treated mice (D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835063&req=5

pone.0153572.g001: rIL-25 administration induces angiogenin-4 expression.CBA/J mice were treated with 0.5 micrograms of recombinant IL-25 (triangle, n = 11) each day for a total of 10 doses over 10 days. Control mice received PBS (open circle, n = 11). Angiogenin-4 relative expression was measured from mouse cecal tissue and normalized with house keeping gene GAPDH and β actin (A). Histological scoring (1 to 5; low to high) for Angiogenin-4 in cecum from mice treated with PBS or rIL-25 (B). Representative IHC staining for angiogenin-4 in samples of cecum tissue from PBS treated (C) or rIL-25 treated mice (D).
Mentions: In order to test if IL-25 induces angiogenin-4 production, we treated CBA/J mice with recombinant IL-25 (rIL-25), and then we measured in mouse ceca the mRNA expression level of angiogenin-4 by qPCR. We found that angiogenin-4 expression was more than 100 fold higher in rIL-25 treated mice than in PBS treated mice (Fig 1A). We confirmed this pattern of expression by performing immunohistochemistry for angiogenin-4 in cecal tissue. Angiogenin-4 protein expression was highly upregulated in rIL-25 treated mice with expression observed in intestinal epithelial cells in the crypts and villi (Fig 1B–1D). We then tested for a dose dependent induction of angiogenin-4 by rIL-25. We found that 4 vs 8 doses of rIL-25 induced a 7-fold and 218-fold increase in angiogenin-4 compared to the PBS control (Fig 2). We concluded that IL-25 induced angiogenin-4 expression in the cecal intestinal epithelium.

Bottom Line: The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues.Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4.IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

View Article: PubMed Central - PubMed

Affiliation: Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia, United States of America.

ABSTRACT
The intestinal surface is directly exposed to both commensal microorganisms as well as pathogens with a single layer of epithelium separating luminal microorganisms from internal tissues. Antimicrobial peptides play a crucial role in allowing epithelial cells to contain in the lumen beneficial and pathogenic microorganisms. The commensal dependent, epithelial produced, Th2 cytokine IL-25 can induce IL-13 and potentially the antimicrobial peptide angiogenin-4. Here we show that IL-13 downstream of IL-25 is required to induce angiogenin-4. IL-25 mediated induction of angiogenin-4 is furthermore not dependent on IL-22 or IL-17.

No MeSH data available.