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The Cultivation of Arabidopsis for Experimental Research Using Commercially Available Peat-Based and Peat-Free Growing Media.

Drake T, Keating M, Summers R, Yochikawa A, Pitman T, Dodd AN - PLoS ONE (2016)

Bottom Line: Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere.Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media.Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, University of Bristol, Bristol Life Sciences Building, 24 Tyndall Avenue, Bristol BS8 1TQ, United Kingdom.

ABSTRACT
Experimental research involving Arabidopsis thaliana often involves the quantification of phenotypic traits during cultivation on compost or other growing media. Many commercially-available growing media contain peat, but peat extraction is not sustainable due to its very slow rate of formation. Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere. Here, we compared the experimental performance of Arabidopsis on peat-based and several types of commercially-available peat-free growing media (variously formed from coir, composted bark, wood-fibre, and domestic compost), to provide guidance for reducing peat use in plant sciences research with Arabidopsis. Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media. Arabidopsis performed extremely poorly on coir alone, presumably because this medium was completely nitrate-free. Some peat-free growing media were more susceptible to fungal contamination. We found that autoclaving of control (peat-based) growing media had no effect upon any physiological parameters that we examined, compared with non-autoclaved control growing media, under our experimental conditions. Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used. This may be because standard laboratory protocols and growth conditions for Arabidopsis are optimized for peat-based media. However, during the vegetative growth phase several phenotypic traits were comparable between plants cultivated on peat-based and some peat-free media, suggesting that under certain circumstances peat-free media can be suitable for phenotypic analysis of Arabidopsis.

No MeSH data available.


Related in: MedlinePlus

Yield and germination of seed from Arabidopsis cultivated on peat-based and peat-free growing media.(a) Seed yield per plant (N = 3; Col-0 P < 0.001; L. er. P < 0.001; Welch’s test followed by post-hoc Games-Howell analyis); (b) Seed viability expressed as the mean proportion of seed germinating from each of 3 parent plants. In some instances, s.e.m. is zero because germination was 100% across seed derived from all parent plants. The experiment included a comparison of native (N) and autoclaved (A) media.
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pone.0153625.g006: Yield and germination of seed from Arabidopsis cultivated on peat-based and peat-free growing media.(a) Seed yield per plant (N = 3; Col-0 P < 0.001; L. er. P < 0.001; Welch’s test followed by post-hoc Games-Howell analyis); (b) Seed viability expressed as the mean proportion of seed germinating from each of 3 parent plants. In some instances, s.e.m. is zero because germination was 100% across seed derived from all parent plants. The experiment included a comparison of native (N) and autoclaved (A) media.

Mentions: Experimental research with Arabidopsis often requires the reliable generation of large quantities of high quality seed. Therefore, we assessed the quality and quantity of seed that was produced following the cultivation of Arabidopsis on several peat-free growing media. Col-0 plants produced significantly fewer seeds after cultivation on all peat-free media compared with the Levington peat-based control (Fig 6A). L. er. plants produced significantly fewer seed when grown on coir (Fig 6A). Interestingly, L. er. plants cultivated on wood-based growing media (Westland, Sylvamix) produced quantities of seed that were comparable with the peat-based control when the media was not autoclaved, but seed yield was reduced significantly on autoclaved wood-based growing media for L. er. (Fig 6A). No seed was obtained from Col-0 or L. er. plants cultivated on autoclaved Westland wood-based media (Fig 6A). Although numerically more seed was obtained from both background lines when cultivated on Levington peat-based media that was autoclaved relative to non-autoclaved Levington media, this was not statistically significant (Fig 6A).


The Cultivation of Arabidopsis for Experimental Research Using Commercially Available Peat-Based and Peat-Free Growing Media.

Drake T, Keating M, Summers R, Yochikawa A, Pitman T, Dodd AN - PLoS ONE (2016)

Yield and germination of seed from Arabidopsis cultivated on peat-based and peat-free growing media.(a) Seed yield per plant (N = 3; Col-0 P < 0.001; L. er. P < 0.001; Welch’s test followed by post-hoc Games-Howell analyis); (b) Seed viability expressed as the mean proportion of seed germinating from each of 3 parent plants. In some instances, s.e.m. is zero because germination was 100% across seed derived from all parent plants. The experiment included a comparison of native (N) and autoclaved (A) media.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835059&req=5

pone.0153625.g006: Yield and germination of seed from Arabidopsis cultivated on peat-based and peat-free growing media.(a) Seed yield per plant (N = 3; Col-0 P < 0.001; L. er. P < 0.001; Welch’s test followed by post-hoc Games-Howell analyis); (b) Seed viability expressed as the mean proportion of seed germinating from each of 3 parent plants. In some instances, s.e.m. is zero because germination was 100% across seed derived from all parent plants. The experiment included a comparison of native (N) and autoclaved (A) media.
Mentions: Experimental research with Arabidopsis often requires the reliable generation of large quantities of high quality seed. Therefore, we assessed the quality and quantity of seed that was produced following the cultivation of Arabidopsis on several peat-free growing media. Col-0 plants produced significantly fewer seeds after cultivation on all peat-free media compared with the Levington peat-based control (Fig 6A). L. er. plants produced significantly fewer seed when grown on coir (Fig 6A). Interestingly, L. er. plants cultivated on wood-based growing media (Westland, Sylvamix) produced quantities of seed that were comparable with the peat-based control when the media was not autoclaved, but seed yield was reduced significantly on autoclaved wood-based growing media for L. er. (Fig 6A). No seed was obtained from Col-0 or L. er. plants cultivated on autoclaved Westland wood-based media (Fig 6A). Although numerically more seed was obtained from both background lines when cultivated on Levington peat-based media that was autoclaved relative to non-autoclaved Levington media, this was not statistically significant (Fig 6A).

Bottom Line: Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere.Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media.Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, University of Bristol, Bristol Life Sciences Building, 24 Tyndall Avenue, Bristol BS8 1TQ, United Kingdom.

ABSTRACT
Experimental research involving Arabidopsis thaliana often involves the quantification of phenotypic traits during cultivation on compost or other growing media. Many commercially-available growing media contain peat, but peat extraction is not sustainable due to its very slow rate of formation. Moreover, peat extraction reduces peatland biodiversity and releases stored carbon and methane into the atmosphere. Here, we compared the experimental performance of Arabidopsis on peat-based and several types of commercially-available peat-free growing media (variously formed from coir, composted bark, wood-fibre, and domestic compost), to provide guidance for reducing peat use in plant sciences research with Arabidopsis. Arabidopsis biomass accumulation and seed yield were reduced by cultivation on several types of peat-free growing media. Arabidopsis performed extremely poorly on coir alone, presumably because this medium was completely nitrate-free. Some peat-free growing media were more susceptible to fungal contamination. We found that autoclaving of control (peat-based) growing media had no effect upon any physiological parameters that we examined, compared with non-autoclaved control growing media, under our experimental conditions. Overall, we conclude that Arabidopsis performs best when cultivated on peat-based growing media because seed yield was almost always reduced when peat-free media were used. This may be because standard laboratory protocols and growth conditions for Arabidopsis are optimized for peat-based media. However, during the vegetative growth phase several phenotypic traits were comparable between plants cultivated on peat-based and some peat-free media, suggesting that under certain circumstances peat-free media can be suitable for phenotypic analysis of Arabidopsis.

No MeSH data available.


Related in: MedlinePlus