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Transcriptome Analysis Revealed Changes of Multiple Genes Involved in Haliotis discus hannai Innate Immunity during Vibrio parahemolyticus Infection.

Nam BH, Jung M, Subramaniyam S, Yoo SI, Markkandan K, Moon JY, Kim YO, Kim DG, An CM, Shin Y, Jung HJ, Park JH - PLoS ONE (2016)

Bottom Line: A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes.Mostly, these groups of genes were dominated in hemocytes compared to other tissues.This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.

ABSTRACT
Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It's therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

No MeSH data available.


Related in: MedlinePlus

Heat map of mitochondrial genes expressed (2-fold) in H. discus hannai transcriptome.
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pone.0153474.g004: Heat map of mitochondrial genes expressed (2-fold) in H. discus hannai transcriptome.

Mentions: Mitochondria emerge as a key component in host innate immune defense, as this organelle is a major cellular source of ROS, which contribute in many ways to host defense against pathogens. We performed an in-house coverage analysis of our infected transcriptome profile with the complete H. discus hannai mitochondrial genome published recently by our group [25]. The results are presented as total number of reads matching with the genes. Overall, 96.57% (16,307 bp) of the mitochondrial genome was mapped with our transcriptome data. Amongst, 13 coding genes, 2 rRNAs and 3 tRNAs showed 100% coverage with the reads. In contrast, the tRNAs varied enormously in their coverage levels. However, when comparing normalized expression, the most highly expressed genes were nd4L and nd6, especially in gill. Five genes had low expression in hepatopancreas, namely nd4, nd4L, nd6, rrn12 and rrn16. The genes encoding subunits of the respiratory complexes (nd1, nd2, nd3, cytb, cox1 to cox3, atp6 and atp8) showed low expression in all tissues and after normalization (Fig 4).


Transcriptome Analysis Revealed Changes of Multiple Genes Involved in Haliotis discus hannai Innate Immunity during Vibrio parahemolyticus Infection.

Nam BH, Jung M, Subramaniyam S, Yoo SI, Markkandan K, Moon JY, Kim YO, Kim DG, An CM, Shin Y, Jung HJ, Park JH - PLoS ONE (2016)

Heat map of mitochondrial genes expressed (2-fold) in H. discus hannai transcriptome.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835058&req=5

pone.0153474.g004: Heat map of mitochondrial genes expressed (2-fold) in H. discus hannai transcriptome.
Mentions: Mitochondria emerge as a key component in host innate immune defense, as this organelle is a major cellular source of ROS, which contribute in many ways to host defense against pathogens. We performed an in-house coverage analysis of our infected transcriptome profile with the complete H. discus hannai mitochondrial genome published recently by our group [25]. The results are presented as total number of reads matching with the genes. Overall, 96.57% (16,307 bp) of the mitochondrial genome was mapped with our transcriptome data. Amongst, 13 coding genes, 2 rRNAs and 3 tRNAs showed 100% coverage with the reads. In contrast, the tRNAs varied enormously in their coverage levels. However, when comparing normalized expression, the most highly expressed genes were nd4L and nd6, especially in gill. Five genes had low expression in hepatopancreas, namely nd4, nd4L, nd6, rrn12 and rrn16. The genes encoding subunits of the respiratory complexes (nd1, nd2, nd3, cytb, cox1 to cox3, atp6 and atp8) showed low expression in all tissues and after normalization (Fig 4).

Bottom Line: A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes.Mostly, these groups of genes were dominated in hemocytes compared to other tissues.This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.

ABSTRACT
Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It's therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

No MeSH data available.


Related in: MedlinePlus