Limits...
Transcriptome Analysis Revealed Changes of Multiple Genes Involved in Haliotis discus hannai Innate Immunity during Vibrio parahemolyticus Infection.

Nam BH, Jung M, Subramaniyam S, Yoo SI, Markkandan K, Moon JY, Kim YO, Kim DG, An CM, Shin Y, Jung HJ, Park JH - PLoS ONE (2016)

Bottom Line: A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes.Mostly, these groups of genes were dominated in hemocytes compared to other tissues.This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.

ABSTRACT
Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It's therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

No MeSH data available.


Related in: MedlinePlus

Differentially expressed genes (DEGs) upon V. parahemolyticus infection.Venn diagrams show DEGs of (A) non-infected and (B) V. parahemolyticus infected. The numbers shown in brackets correspond to the nucleotide sequences available in GenBank. (C) Tissue specific histogram of up and down regulated transcripts. (D) Heat map of transcripts with expression change more than 2-fold in each tissues.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835058&req=5

pone.0153474.g003: Differentially expressed genes (DEGs) upon V. parahemolyticus infection.Venn diagrams show DEGs of (A) non-infected and (B) V. parahemolyticus infected. The numbers shown in brackets correspond to the nucleotide sequences available in GenBank. (C) Tissue specific histogram of up and down regulated transcripts. (D) Heat map of transcripts with expression change more than 2-fold in each tissues.

Mentions: Crosstalk between stressors and their stress response have been extensively reported in abalones [5, 8, 27–32]. To obtain a general view of DEG expression patterns, pairwise comparison of expression abundance in the RNA-seq data of four library sets were first conducted in accordance with GenBank as described in methods. A Venn diagram in Fig 2B describes, overall, 10,575 transcripts expression were altered in all four tissues after exposure to V. parahemolyticus infection. Among these tissues hemocytes, hepatopancreas, mantle and gill, a total of 9,726, 332, 83 and 170 transcripts, respectively, were found to be differentially expressed (Fig 3).


Transcriptome Analysis Revealed Changes of Multiple Genes Involved in Haliotis discus hannai Innate Immunity during Vibrio parahemolyticus Infection.

Nam BH, Jung M, Subramaniyam S, Yoo SI, Markkandan K, Moon JY, Kim YO, Kim DG, An CM, Shin Y, Jung HJ, Park JH - PLoS ONE (2016)

Differentially expressed genes (DEGs) upon V. parahemolyticus infection.Venn diagrams show DEGs of (A) non-infected and (B) V. parahemolyticus infected. The numbers shown in brackets correspond to the nucleotide sequences available in GenBank. (C) Tissue specific histogram of up and down regulated transcripts. (D) Heat map of transcripts with expression change more than 2-fold in each tissues.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835058&req=5

pone.0153474.g003: Differentially expressed genes (DEGs) upon V. parahemolyticus infection.Venn diagrams show DEGs of (A) non-infected and (B) V. parahemolyticus infected. The numbers shown in brackets correspond to the nucleotide sequences available in GenBank. (C) Tissue specific histogram of up and down regulated transcripts. (D) Heat map of transcripts with expression change more than 2-fold in each tissues.
Mentions: Crosstalk between stressors and their stress response have been extensively reported in abalones [5, 8, 27–32]. To obtain a general view of DEG expression patterns, pairwise comparison of expression abundance in the RNA-seq data of four library sets were first conducted in accordance with GenBank as described in methods. A Venn diagram in Fig 2B describes, overall, 10,575 transcripts expression were altered in all four tissues after exposure to V. parahemolyticus infection. Among these tissues hemocytes, hepatopancreas, mantle and gill, a total of 9,726, 332, 83 and 170 transcripts, respectively, were found to be differentially expressed (Fig 3).

Bottom Line: A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes.Mostly, these groups of genes were dominated in hemocytes compared to other tissues.This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Division, Aquaculture Industry Department, National Fisheries Research and Development Institute, Busan 619-902, Republic of Korea.

ABSTRACT
Abalone (Haliotis discus hannai) is one of the most valuable marine aquatic species in Korea, Japan and China. Tremendous exposure to bacterial infection is common in aquaculture environment, especially by Vibrio sp. infections. It's therefore necessary and urgent to understand the mechanism of H. discus hannai host defense against Vibrio parahemolyticus infection. However studies on its immune system are hindered by the lack of genomic resources. In the present study, we sequenced the transcriptome of control and bacterial challenged H. discus hannai tissues. Totally, 138 MB of reference transcriptome were obtained from de novo assembly of 34 GB clean bases from ten different libraries and annotated with the biological terms (GO and KEGG). A total of 10,575 transcripts exhibiting the differentially expression at least one pair of comparison and the functional annotations highlight genes related to immune response, cell adhesion, immune regulators, redox molecules and mitochondrial coding genes. Mostly, these groups of genes were dominated in hemocytes compared to other tissues. This work is a prerequisite for the identification of those physiological traits controlling H. discus hannai ability to survive against Vibrio infection.

No MeSH data available.


Related in: MedlinePlus