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Midkine and Pleiotrophin Concentrations in Amniotic Fluid in Healthy and Complicated Pregnancies.

Jee YH, Lebenthal Y, Chaemsaithong P, Yan G, Peran I, Wellstein A, Romero R, Baron J - PLoS ONE (2016)

Bottom Line: Midkine (MDK) and pleiotrophin (PTN) are heparin-binding growth factors that, in rodents, are highly expressed in early life and decrease to undetectable levels by adulthood.We found that both of these growth factors could be readily measured in AF and that the concentrations were higher than most cytokines previously reported in AF.Both MDK and PTN concentrations were found to be lower in pregnancies that were complicated by chorioamnionitis at term, raising the possibility that these growth factors might be useful as markers for infection.

View Article: PubMed Central - PubMed

Affiliation: Section on Growth and Development, Program on Developmental Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America.

ABSTRACT

Background: Midkine (MDK) and pleiotrophin (PTN) are heparin-binding growth factors that, in rodents, are highly expressed in early life and decrease to undetectable levels by adulthood. The potential roles of MDK and PTN in human growth and development are not completely elucidated.

Method and findings: To delineate the role of MDK and PTN in human development, we developed high sensitivity assays to measure their concentrations in amniotic fluid (AF) at various gestational ages in both healthy and complicated pregnancies. We found that both of these growth factors could be readily measured in AF and that the concentrations were higher than most cytokines previously reported in AF.

Conclusion: The concentration of MDK but not that of PTN declined with gestational age. Both MDK and PTN concentrations were found to be lower in pregnancies that were complicated by chorioamnionitis at term, raising the possibility that these growth factors might be useful as markers for infection.

No MeSH data available.


Related in: MedlinePlus

Plasma MDK concentrations during pregnancy and in non-pregnant healthy female controls.Plasma MDK concentrations were similar among non-pregnant healthy women, normal preterm pregnancies in the absence of labor, preterm in labor, PPROM, term not in labor, and term in labor. Data are presented as mean ± SEM.
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pone.0153325.g003: Plasma MDK concentrations during pregnancy and in non-pregnant healthy female controls.Plasma MDK concentrations were similar among non-pregnant healthy women, normal preterm pregnancies in the absence of labor, preterm in labor, PPROM, term not in labor, and term in labor. Data are presented as mean ± SEM.

Mentions: The average age of the pregnant women at time of plasma sampling was similar to that of the non-pregnant healthy controls [27.6 years (18–40 years) vs. 25.2 years (17–37 years), P = 0.18]. Plasma MDK concentrations did not significantly differ between the pregnant women and non-pregnant age-matched controls (0.19 ± 0.01 ng/ml vs. 0.16 ± 0.02 ng/ml, P = 0.79). No significant differences in plasma MDK concentrations were found among non-pregnant healthy women, normal mid-term pregnancy, preterm in labor, PPROM, term without labor, and term with labor (Fig 3).


Midkine and Pleiotrophin Concentrations in Amniotic Fluid in Healthy and Complicated Pregnancies.

Jee YH, Lebenthal Y, Chaemsaithong P, Yan G, Peran I, Wellstein A, Romero R, Baron J - PLoS ONE (2016)

Plasma MDK concentrations during pregnancy and in non-pregnant healthy female controls.Plasma MDK concentrations were similar among non-pregnant healthy women, normal preterm pregnancies in the absence of labor, preterm in labor, PPROM, term not in labor, and term in labor. Data are presented as mean ± SEM.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835047&req=5

pone.0153325.g003: Plasma MDK concentrations during pregnancy and in non-pregnant healthy female controls.Plasma MDK concentrations were similar among non-pregnant healthy women, normal preterm pregnancies in the absence of labor, preterm in labor, PPROM, term not in labor, and term in labor. Data are presented as mean ± SEM.
Mentions: The average age of the pregnant women at time of plasma sampling was similar to that of the non-pregnant healthy controls [27.6 years (18–40 years) vs. 25.2 years (17–37 years), P = 0.18]. Plasma MDK concentrations did not significantly differ between the pregnant women and non-pregnant age-matched controls (0.19 ± 0.01 ng/ml vs. 0.16 ± 0.02 ng/ml, P = 0.79). No significant differences in plasma MDK concentrations were found among non-pregnant healthy women, normal mid-term pregnancy, preterm in labor, PPROM, term without labor, and term with labor (Fig 3).

Bottom Line: Midkine (MDK) and pleiotrophin (PTN) are heparin-binding growth factors that, in rodents, are highly expressed in early life and decrease to undetectable levels by adulthood.We found that both of these growth factors could be readily measured in AF and that the concentrations were higher than most cytokines previously reported in AF.Both MDK and PTN concentrations were found to be lower in pregnancies that were complicated by chorioamnionitis at term, raising the possibility that these growth factors might be useful as markers for infection.

View Article: PubMed Central - PubMed

Affiliation: Section on Growth and Development, Program on Developmental Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, United States of America.

ABSTRACT

Background: Midkine (MDK) and pleiotrophin (PTN) are heparin-binding growth factors that, in rodents, are highly expressed in early life and decrease to undetectable levels by adulthood. The potential roles of MDK and PTN in human growth and development are not completely elucidated.

Method and findings: To delineate the role of MDK and PTN in human development, we developed high sensitivity assays to measure their concentrations in amniotic fluid (AF) at various gestational ages in both healthy and complicated pregnancies. We found that both of these growth factors could be readily measured in AF and that the concentrations were higher than most cytokines previously reported in AF.

Conclusion: The concentration of MDK but not that of PTN declined with gestational age. Both MDK and PTN concentrations were found to be lower in pregnancies that were complicated by chorioamnionitis at term, raising the possibility that these growth factors might be useful as markers for infection.

No MeSH data available.


Related in: MedlinePlus