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Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation.

Yi L, Wang Y, Ma Z, Lin HX, Xu B, Grenier D, Fan HJ, Lu CP - Vet. Res. (2016)

Bottom Line: Biofilm formation by this bacterium has been previously reported.In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus.Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria.

View Article: PubMed Central - PubMed

Affiliation: Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Streptococcus equi ssp. zooepidemicus (S. equi spp. zooepidemicus) is an opportunistic pathogen that causes major economic losses in the swine industry in China and is also a threat for human health. Biofilm formation by this bacterium has been previously reported. In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus. Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria. The immunogenicity and protective efficacy of the S. equi spp. zooepidemicus immunoreactive GroEL chaperone protein was further investigated in mice. The protein was expressed in vivo and elicited high antibody titers following S. equi spp. zooepidemicus infections of mice. An animal challenge experiment with S. equi spp. zooepidemicus showed that 75% of mice immunized with the GroEL protein were protected. Using in vitro biofilm inhibition assays, evidence was obtained that the chaperonin GroEL may represent a promising target for the prevention and treatment of persistent S. equi spp. zooepidemicus biofilm infections. In summary, our results suggest that the recombinant GroEL protein, which is involved in biofilm formation, may efficiently stimulate an immune response, which protects against S. equi spp. zooepidemicus infections. It may therefore be a candidate of interest to be included in vaccines against S. equi spp. zooepidemicus infections.

No MeSH data available.


Related in: MedlinePlus

Protective efficacy by vaccination in mice. Mice were injected with S. equispp.zooepidemicus ATCC35246 and mortality was recorded daily for 7 days. Mice in the non-immunized group died 24 to 48 h after the challenge, and the mortality rate reached 100%. Mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine died 48 to 72 h after the challenge with S. equi spp. zooepidemicus and 75% survived 7 days post-infection for these two groups. Significant differences in survival were noted, log rank test, P < 0.05. Compared with the non-immunized group, the mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine had higher survival rates (P < 0.05).
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Fig4: Protective efficacy by vaccination in mice. Mice were injected with S. equispp.zooepidemicus ATCC35246 and mortality was recorded daily for 7 days. Mice in the non-immunized group died 24 to 48 h after the challenge, and the mortality rate reached 100%. Mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine died 48 to 72 h after the challenge with S. equi spp. zooepidemicus and 75% survived 7 days post-infection for these two groups. Significant differences in survival were noted, log rank test, P < 0.05. Compared with the non-immunized group, the mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine had higher survival rates (P < 0.05).

Mentions: Immunized and non-immunized mice were monitored daily for 7 days following a challenge with S. equi spp. zooepidemicus. In the non-immunized group, the first death of mice occurred 24 h after the challenge, and the mortality rate reached 100% within 48 h. In the groups immunized with rGroEL or the inactivated bacterial vaccine, only 2 out of 8 mice (25%) died following the challenge with S. equi spp. zooepidemicus. More specifically, the deaths occurred at day 3 post-injection. The immunoprotection rate for these two groups was 75% in both cases (Figure 4). Compared with the non-immunized group,the mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine had higher survival rates (P < 0.05). No significant differences in survival rates were found between the group immunized with rGroEL and the group inactivated S. equi spp. zooepidemicus vaccine (P > 0.05).Figure 4


Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation.

Yi L, Wang Y, Ma Z, Lin HX, Xu B, Grenier D, Fan HJ, Lu CP - Vet. Res. (2016)

Protective efficacy by vaccination in mice. Mice were injected with S. equispp.zooepidemicus ATCC35246 and mortality was recorded daily for 7 days. Mice in the non-immunized group died 24 to 48 h after the challenge, and the mortality rate reached 100%. Mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine died 48 to 72 h after the challenge with S. equi spp. zooepidemicus and 75% survived 7 days post-infection for these two groups. Significant differences in survival were noted, log rank test, P < 0.05. Compared with the non-immunized group, the mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine had higher survival rates (P < 0.05).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4834820&req=5

Fig4: Protective efficacy by vaccination in mice. Mice were injected with S. equispp.zooepidemicus ATCC35246 and mortality was recorded daily for 7 days. Mice in the non-immunized group died 24 to 48 h after the challenge, and the mortality rate reached 100%. Mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine died 48 to 72 h after the challenge with S. equi spp. zooepidemicus and 75% survived 7 days post-infection for these two groups. Significant differences in survival were noted, log rank test, P < 0.05. Compared with the non-immunized group, the mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine had higher survival rates (P < 0.05).
Mentions: Immunized and non-immunized mice were monitored daily for 7 days following a challenge with S. equi spp. zooepidemicus. In the non-immunized group, the first death of mice occurred 24 h after the challenge, and the mortality rate reached 100% within 48 h. In the groups immunized with rGroEL or the inactivated bacterial vaccine, only 2 out of 8 mice (25%) died following the challenge with S. equi spp. zooepidemicus. More specifically, the deaths occurred at day 3 post-injection. The immunoprotection rate for these two groups was 75% in both cases (Figure 4). Compared with the non-immunized group,the mice in the groups immunized with rGroEL or the inactivated S. equi spp. zooepidemicus vaccine had higher survival rates (P < 0.05). No significant differences in survival rates were found between the group immunized with rGroEL and the group inactivated S. equi spp. zooepidemicus vaccine (P > 0.05).Figure 4

Bottom Line: Biofilm formation by this bacterium has been previously reported.In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus.Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria.

View Article: PubMed Central - PubMed

Affiliation: Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China.

ABSTRACT
Streptococcus equi ssp. zooepidemicus (S. equi spp. zooepidemicus) is an opportunistic pathogen that causes major economic losses in the swine industry in China and is also a threat for human health. Biofilm formation by this bacterium has been previously reported. In this study, we used an immunoproteomic approach to search for immunogenic proteins expressed by biofilm-grown S. equi spp. zooepidemicus. Seventeen immunoreactive proteins were found, of which nine common immunoreactive proteins were identified in planktonic and biofilm-grown bacteria. The immunogenicity and protective efficacy of the S. equi spp. zooepidemicus immunoreactive GroEL chaperone protein was further investigated in mice. The protein was expressed in vivo and elicited high antibody titers following S. equi spp. zooepidemicus infections of mice. An animal challenge experiment with S. equi spp. zooepidemicus showed that 75% of mice immunized with the GroEL protein were protected. Using in vitro biofilm inhibition assays, evidence was obtained that the chaperonin GroEL may represent a promising target for the prevention and treatment of persistent S. equi spp. zooepidemicus biofilm infections. In summary, our results suggest that the recombinant GroEL protein, which is involved in biofilm formation, may efficiently stimulate an immune response, which protects against S. equi spp. zooepidemicus infections. It may therefore be a candidate of interest to be included in vaccines against S. equi spp. zooepidemicus infections.

No MeSH data available.


Related in: MedlinePlus