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LIM Homeobox Domain 2 Is Required for Corneal Epithelial Homeostasis.

Sartaj R, Chee RI, Yang J, Wan P, Liu A, Guaiquil V, Fuchs E, Rosenblatt MI - Stem Cells (2016)

Bottom Line: Immunodetection on corneal sections were used to visualize conjunctivalization, a sign of limbal barrier failure.Cell based assays showed that Lhx2cKO derived corneal epithelial cells have a significantly lower capacity to form colonies over time and delayed wound-healing recovery when compared to wildtype cells.We conclude that Lhx2 is required for maintenance of the corneal epithelial cell compartment and the limbal barrier.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, Chicago, Illinois, USA.

No MeSH data available.


Related in: MedlinePlus

Lhx2 contributes to maintenance of the limbal barrier.Detection of debridement‐induced conjunctivalization of the cornea in Lhx2cKO mice was visible in eye sections stained with anti Keratin 15 (K15) antibody (red) and Periodic Acid Schiff (PAS) staining which is specific for goblet cells. In normal (A,a) and debrided WT corneas (B, b) no changes in morphology were observed, and K15 and goblet cells were detected only in the conjunctiva. In non‐debrided Lhx2cKO cornea a similar pattern is observed with no alteration in the cornea (C,c), but upon epithelial debridement, goblet cells and K15 positive staining were present in both the conjunctiva and the cornea (D,d). Furthermore, some Lhx2cKO mice developed neovascularization (NV) (E, e) or neovascularization plus persistent epithelial defects (PED) (F, f). In the immunofluorescence images, selected areas (small box) are enlarged for better visualization of K15. Black arrows indicate goblet cells. c = cornea; cj = conjunctiva. Scale bars: A–F = 1000μm; a,b,c,e,f  = 200μm; and d = 100μm.
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stem2257-fig-0006: Lhx2 contributes to maintenance of the limbal barrier.Detection of debridement‐induced conjunctivalization of the cornea in Lhx2cKO mice was visible in eye sections stained with anti Keratin 15 (K15) antibody (red) and Periodic Acid Schiff (PAS) staining which is specific for goblet cells. In normal (A,a) and debrided WT corneas (B, b) no changes in morphology were observed, and K15 and goblet cells were detected only in the conjunctiva. In non‐debrided Lhx2cKO cornea a similar pattern is observed with no alteration in the cornea (C,c), but upon epithelial debridement, goblet cells and K15 positive staining were present in both the conjunctiva and the cornea (D,d). Furthermore, some Lhx2cKO mice developed neovascularization (NV) (E, e) or neovascularization plus persistent epithelial defects (PED) (F, f). In the immunofluorescence images, selected areas (small box) are enlarged for better visualization of K15. Black arrows indicate goblet cells. c = cornea; cj = conjunctiva. Scale bars: A–F = 1000μm; a,b,c,e,f  = 200μm; and d = 100μm.

Mentions: In the normal eye, the limbus separates the avascular cornea from the conjunctiva. The limbus is rich in stem cells and highly vascularized and by mechanisms that are not totally understood, it prevents blood vessels penetrating into the cornea. We observed that after epithelial debridement, some corneas in our Lhx2cKO population showed signs of neovascularization. Therefore, we investigated whether WT and Lhx2cKO corneas showed histological signs of neovascularization and conjunctivalization by looking for specific markers, such as presence of goblet cells (normally only present in the conjunctiva) using PAS and keratin 15 (K15), a marker of conjunctival and limbal epithelial tissue in mice 21. Nondebrided and after the third debridement corneas from WT and Lhx2cKO mice were sectioned and stained, including the cornea, bulbar, forniceal, and palpebral conjunctiva. Higher power images are shown (large white boxes) for immunofluorescence and PAS (Fig. 6).


LIM Homeobox Domain 2 Is Required for Corneal Epithelial Homeostasis.

Sartaj R, Chee RI, Yang J, Wan P, Liu A, Guaiquil V, Fuchs E, Rosenblatt MI - Stem Cells (2016)

Lhx2 contributes to maintenance of the limbal barrier.Detection of debridement‐induced conjunctivalization of the cornea in Lhx2cKO mice was visible in eye sections stained with anti Keratin 15 (K15) antibody (red) and Periodic Acid Schiff (PAS) staining which is specific for goblet cells. In normal (A,a) and debrided WT corneas (B, b) no changes in morphology were observed, and K15 and goblet cells were detected only in the conjunctiva. In non‐debrided Lhx2cKO cornea a similar pattern is observed with no alteration in the cornea (C,c), but upon epithelial debridement, goblet cells and K15 positive staining were present in both the conjunctiva and the cornea (D,d). Furthermore, some Lhx2cKO mice developed neovascularization (NV) (E, e) or neovascularization plus persistent epithelial defects (PED) (F, f). In the immunofluorescence images, selected areas (small box) are enlarged for better visualization of K15. Black arrows indicate goblet cells. c = cornea; cj = conjunctiva. Scale bars: A–F = 1000μm; a,b,c,e,f  = 200μm; and d = 100μm.
© Copyright Policy - creativeCommonsBy-nc-nd
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4834794&req=5

stem2257-fig-0006: Lhx2 contributes to maintenance of the limbal barrier.Detection of debridement‐induced conjunctivalization of the cornea in Lhx2cKO mice was visible in eye sections stained with anti Keratin 15 (K15) antibody (red) and Periodic Acid Schiff (PAS) staining which is specific for goblet cells. In normal (A,a) and debrided WT corneas (B, b) no changes in morphology were observed, and K15 and goblet cells were detected only in the conjunctiva. In non‐debrided Lhx2cKO cornea a similar pattern is observed with no alteration in the cornea (C,c), but upon epithelial debridement, goblet cells and K15 positive staining were present in both the conjunctiva and the cornea (D,d). Furthermore, some Lhx2cKO mice developed neovascularization (NV) (E, e) or neovascularization plus persistent epithelial defects (PED) (F, f). In the immunofluorescence images, selected areas (small box) are enlarged for better visualization of K15. Black arrows indicate goblet cells. c = cornea; cj = conjunctiva. Scale bars: A–F = 1000μm; a,b,c,e,f  = 200μm; and d = 100μm.
Mentions: In the normal eye, the limbus separates the avascular cornea from the conjunctiva. The limbus is rich in stem cells and highly vascularized and by mechanisms that are not totally understood, it prevents blood vessels penetrating into the cornea. We observed that after epithelial debridement, some corneas in our Lhx2cKO population showed signs of neovascularization. Therefore, we investigated whether WT and Lhx2cKO corneas showed histological signs of neovascularization and conjunctivalization by looking for specific markers, such as presence of goblet cells (normally only present in the conjunctiva) using PAS and keratin 15 (K15), a marker of conjunctival and limbal epithelial tissue in mice 21. Nondebrided and after the third debridement corneas from WT and Lhx2cKO mice were sectioned and stained, including the cornea, bulbar, forniceal, and palpebral conjunctiva. Higher power images are shown (large white boxes) for immunofluorescence and PAS (Fig. 6).

Bottom Line: Immunodetection on corneal sections were used to visualize conjunctivalization, a sign of limbal barrier failure.Cell based assays showed that Lhx2cKO derived corneal epithelial cells have a significantly lower capacity to form colonies over time and delayed wound-healing recovery when compared to wildtype cells.We conclude that Lhx2 is required for maintenance of the corneal epithelial cell compartment and the limbal barrier.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology and Visual Sciences, Illinois Eye and Ear Infirmary, University of Illinois at Chicago, Chicago, Illinois, USA.

No MeSH data available.


Related in: MedlinePlus