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Monitoring the progression of renal fibrosis by T2-weighted signal intensity and diffusion weighted magnetic resonance imaging in cisplatin induced rat models.

Wu HH, Jia HR, Zhang Y, Liu L, Xu DB, Sun HR - Chin. Med. J. (2015)

Bottom Line: OM revealed remarkable hyperintense and broadened before it became an obscure thread, while CO demonstrated moderate hyperintense and IM didn't show significant change on T2WI.On all three stripes, ADC values decreased firstly then kept increasing since the 4 th day; f values decreased on all stripes; D values had a tendency to increase with fluctuations but the changes didn't achieve statistical significance; DFNx01 values increased at the 2 nd day then tended to be steady thereafter.All of T2-weighted SI, ADC, and biexponential models parameters vary during fibrotic process; biexponential model is superior to monoexponential model in separating changes of microperfusion together with tubular flow from pure diffusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Tianjin Medical University Hospital, Tianjin, China.

ABSTRACT

Background: Diffusion weighted imaging (DWI), with the applying of intravoxel incoherent motion model, has showed promising results in obtaining additional information about microperfusion and tubular flow associated with morphologic changes in chronic kidney diseases. The study aims to evaluate the potential of T2-weighted signal intensity (SI) and DWI with mono- and bi-exponential models to reflect the serial changes on cisplatin (CP) induced rat renal fibrosis models.

Methods: Magnetic resonance exams were performed prior to and 2 nd day, 4 th day, 6 th day, 8 th day, 2 nd week, 3 rd week and 4 th week after CP injection at a 3.0T with an animal coil. Besides T2-weighted images (T2WI), DWI of 13 b values from 0 to 1500 s/mm 2 was acquired. Apparent diffusion coefficient (ADC), fluid fraction f, pure diffusivity D and pseudodiffusivity DFNx01 values were calculated. The regions of interest were placed on cortex (CO), outer stripe of the outer medulla (OM) and inner stripe of the outer medulla (OM), parameters were measured and compared among different time points. Five rats were scarified at each time point for pathological examination.

Results: OM revealed remarkable hyperintense and broadened before it became an obscure thread, while CO demonstrated moderate hyperintense and IM didn't show significant change on T2WI. On all three stripes, ADC values decreased firstly then kept increasing since the 4 th day; f values decreased on all stripes; D values had a tendency to increase with fluctuations but the changes didn't achieve statistical significance; DFNx01 values increased at the 2 nd day then tended to be steady thereafter. Pathological findings revealed tubules epitheliums swelling followed by inflammation cells infiltration, interstitial fibrosis was observed since the 2 nd week.

Conclusions: All of T2-weighted SI, ADC, and biexponential models parameters vary during fibrotic process; biexponential model is superior to monoexponential model in separating changes of microperfusion together with tubular flow from pure diffusion.

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Serial pathological changes of the renal parenchyma in rats after CP administration (H and E, ×200). (a) At the 2nd day after CP injection, the epitheliums of renal tubules showed obviously cell swelling; (b) At the 6th day, cell turbidity (yellow arrow) and renal tubular mold could be observed (orange arrow); (c) At the 8th day, inflammation cells infiltrated in renal interstitium (arrow) resulting in cellularity increasing; (d) At the 4th week, massive fibrotic collagen (arrow) within broadened renal interstitial space was demonstrated together with tubular atrophy. CP: Cisplatin.
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Figure 4: Serial pathological changes of the renal parenchyma in rats after CP administration (H and E, ×200). (a) At the 2nd day after CP injection, the epitheliums of renal tubules showed obviously cell swelling; (b) At the 6th day, cell turbidity (yellow arrow) and renal tubular mold could be observed (orange arrow); (c) At the 8th day, inflammation cells infiltrated in renal interstitium (arrow) resulting in cellularity increasing; (d) At the 4th week, massive fibrotic collagen (arrow) within broadened renal interstitial space was demonstrated together with tubular atrophy. CP: Cisplatin.

Mentions: At the 2nd and 4th day after CP injection, the epitheliums of renal tubules showed obviously cell swelling and tubules narrowing, and OM stripes which was consisting of proximal convoluted tubules demonstrated the most remarkable changes [refer to the acute stage, Figure 4a]. During the 6th and 8th day, cell swelling relieved while cell turbidity and renal tubular mold could be observed, massive inflammation cells began to infiltrate in the interstitium [refer to the chronic stage, Figure 4b and c]. Renal interstitial fibrosis was initially observed at 2nd week; tubular atrophy and necrosis, broadened interstitial space with proliferation of myofibroblasts and cellular density increasing could be demonstrated throughout the fibrotic process after the 3rd week [refer to the fibrosis stage, Figure 4d].


Monitoring the progression of renal fibrosis by T2-weighted signal intensity and diffusion weighted magnetic resonance imaging in cisplatin induced rat models.

Wu HH, Jia HR, Zhang Y, Liu L, Xu DB, Sun HR - Chin. Med. J. (2015)

Serial pathological changes of the renal parenchyma in rats after CP administration (H and E, ×200). (a) At the 2nd day after CP injection, the epitheliums of renal tubules showed obviously cell swelling; (b) At the 6th day, cell turbidity (yellow arrow) and renal tubular mold could be observed (orange arrow); (c) At the 8th day, inflammation cells infiltrated in renal interstitium (arrow) resulting in cellularity increasing; (d) At the 4th week, massive fibrotic collagen (arrow) within broadened renal interstitial space was demonstrated together with tubular atrophy. CP: Cisplatin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834773&req=5

Figure 4: Serial pathological changes of the renal parenchyma in rats after CP administration (H and E, ×200). (a) At the 2nd day after CP injection, the epitheliums of renal tubules showed obviously cell swelling; (b) At the 6th day, cell turbidity (yellow arrow) and renal tubular mold could be observed (orange arrow); (c) At the 8th day, inflammation cells infiltrated in renal interstitium (arrow) resulting in cellularity increasing; (d) At the 4th week, massive fibrotic collagen (arrow) within broadened renal interstitial space was demonstrated together with tubular atrophy. CP: Cisplatin.
Mentions: At the 2nd and 4th day after CP injection, the epitheliums of renal tubules showed obviously cell swelling and tubules narrowing, and OM stripes which was consisting of proximal convoluted tubules demonstrated the most remarkable changes [refer to the acute stage, Figure 4a]. During the 6th and 8th day, cell swelling relieved while cell turbidity and renal tubular mold could be observed, massive inflammation cells began to infiltrate in the interstitium [refer to the chronic stage, Figure 4b and c]. Renal interstitial fibrosis was initially observed at 2nd week; tubular atrophy and necrosis, broadened interstitial space with proliferation of myofibroblasts and cellular density increasing could be demonstrated throughout the fibrotic process after the 3rd week [refer to the fibrosis stage, Figure 4d].

Bottom Line: OM revealed remarkable hyperintense and broadened before it became an obscure thread, while CO demonstrated moderate hyperintense and IM didn't show significant change on T2WI.On all three stripes, ADC values decreased firstly then kept increasing since the 4 th day; f values decreased on all stripes; D values had a tendency to increase with fluctuations but the changes didn't achieve statistical significance; DFNx01 values increased at the 2 nd day then tended to be steady thereafter.All of T2-weighted SI, ADC, and biexponential models parameters vary during fibrotic process; biexponential model is superior to monoexponential model in separating changes of microperfusion together with tubular flow from pure diffusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology, Tianjin Medical University Hospital, Tianjin, China.

ABSTRACT

Background: Diffusion weighted imaging (DWI), with the applying of intravoxel incoherent motion model, has showed promising results in obtaining additional information about microperfusion and tubular flow associated with morphologic changes in chronic kidney diseases. The study aims to evaluate the potential of T2-weighted signal intensity (SI) and DWI with mono- and bi-exponential models to reflect the serial changes on cisplatin (CP) induced rat renal fibrosis models.

Methods: Magnetic resonance exams were performed prior to and 2 nd day, 4 th day, 6 th day, 8 th day, 2 nd week, 3 rd week and 4 th week after CP injection at a 3.0T with an animal coil. Besides T2-weighted images (T2WI), DWI of 13 b values from 0 to 1500 s/mm 2 was acquired. Apparent diffusion coefficient (ADC), fluid fraction f, pure diffusivity D and pseudodiffusivity DFNx01 values were calculated. The regions of interest were placed on cortex (CO), outer stripe of the outer medulla (OM) and inner stripe of the outer medulla (OM), parameters were measured and compared among different time points. Five rats were scarified at each time point for pathological examination.

Results: OM revealed remarkable hyperintense and broadened before it became an obscure thread, while CO demonstrated moderate hyperintense and IM didn't show significant change on T2WI. On all three stripes, ADC values decreased firstly then kept increasing since the 4 th day; f values decreased on all stripes; D values had a tendency to increase with fluctuations but the changes didn't achieve statistical significance; DFNx01 values increased at the 2 nd day then tended to be steady thereafter. Pathological findings revealed tubules epitheliums swelling followed by inflammation cells infiltration, interstitial fibrosis was observed since the 2 nd week.

Conclusions: All of T2-weighted SI, ADC, and biexponential models parameters vary during fibrotic process; biexponential model is superior to monoexponential model in separating changes of microperfusion together with tubular flow from pure diffusion.

Show MeSH
Related in: MedlinePlus