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Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Liu Q, Huang X, Yang D, Si T, Pan S, Yang F - EXCLI J (2016)

Bottom Line: It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield.In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L.The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Engineering, Hubei University Zhixing College, Wuhan 430011, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

No MeSH data available.


Related in: MedlinePlus

EPS yields of the 19 mutant strains and the control in MRS broth medium after incubation at 37 °C for 24 h (n=3, Mean±SEM)
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T4: EPS yields of the 19 mutant strains and the control in MRS broth medium after incubation at 37 °C for 24 h (n=3, Mean±SEM)

Mentions: The EPS yield of initial strain (control) was 1.05±0.06 g/L (Table 4(Tab. 4)), and the EPS yields of the 19 mutant strains that could increase the milk viscosity significantly were determined to gain the high-yielding mutant strains. Since the EPS yield of the No. 48 strains (2.92±0.05 g/L) was definitely higher than that of the other strains (P < 0.01), they were selected for the objection strain.


Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Liu Q, Huang X, Yang D, Si T, Pan S, Yang F - EXCLI J (2016)

EPS yields of the 19 mutant strains and the control in MRS broth medium after incubation at 37 °C for 24 h (n=3, Mean±SEM)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834753&req=5

T4: EPS yields of the 19 mutant strains and the control in MRS broth medium after incubation at 37 °C for 24 h (n=3, Mean±SEM)
Mentions: The EPS yield of initial strain (control) was 1.05±0.06 g/L (Table 4(Tab. 4)), and the EPS yields of the 19 mutant strains that could increase the milk viscosity significantly were determined to gain the high-yielding mutant strains. Since the EPS yield of the No. 48 strains (2.92±0.05 g/L) was definitely higher than that of the other strains (P < 0.01), they were selected for the objection strain.

Bottom Line: It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield.In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L.The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Engineering, Hubei University Zhixing College, Wuhan 430011, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

No MeSH data available.


Related in: MedlinePlus