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Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Liu Q, Huang X, Yang D, Si T, Pan S, Yang F - EXCLI J (2016)

Bottom Line: It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield.In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L.The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Engineering, Hubei University Zhixing College, Wuhan 430011, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

No MeSH data available.


Related in: MedlinePlus

Experimental design and results of CCD on the extraction process optimization of EPS produced by activated L. acidophilus ATCC (n=3, Mean±SEM).
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T3: Experimental design and results of CCD on the extraction process optimization of EPS produced by activated L. acidophilus ATCC (n=3, Mean±SEM).

Mentions: The optimum ranges of the three factors (alcohol concentration, M/L ratio, and extraction time), which affected the extraction of EPS, were identified by signal factor experiments for the further optimization by CCD. The different alcohol concentrations (50 %, 60 %, 70 %, 80 %, and 95 %), M/L ratios (1:1, 1:2, 1:3, 1:4, and 1:5) and extraction times (6 h, 12 h, 24 h, 36 h, and 48 h) were operated in the signal factor experiments respectively. In each single factor experiment, one factor was changed while the other factors remained constant. The design matrix of the variables in coded units was presented in Table 3(Tab. 3). The model equation for analysis is as the same as Equation 1.


Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Liu Q, Huang X, Yang D, Si T, Pan S, Yang F - EXCLI J (2016)

Experimental design and results of CCD on the extraction process optimization of EPS produced by activated L. acidophilus ATCC (n=3, Mean±SEM).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834753&req=5

T3: Experimental design and results of CCD on the extraction process optimization of EPS produced by activated L. acidophilus ATCC (n=3, Mean±SEM).
Mentions: The optimum ranges of the three factors (alcohol concentration, M/L ratio, and extraction time), which affected the extraction of EPS, were identified by signal factor experiments for the further optimization by CCD. The different alcohol concentrations (50 %, 60 %, 70 %, 80 %, and 95 %), M/L ratios (1:1, 1:2, 1:3, 1:4, and 1:5) and extraction times (6 h, 12 h, 24 h, 36 h, and 48 h) were operated in the signal factor experiments respectively. In each single factor experiment, one factor was changed while the other factors remained constant. The design matrix of the variables in coded units was presented in Table 3(Tab. 3). The model equation for analysis is as the same as Equation 1.

Bottom Line: It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield.In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L.The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Engineering, Hubei University Zhixing College, Wuhan 430011, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

No MeSH data available.


Related in: MedlinePlus