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Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Liu Q, Huang X, Yang D, Si T, Pan S, Yang F - EXCLI J (2016)

Bottom Line: It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield.In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L.The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Engineering, Hubei University Zhixing College, Wuhan 430011, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

No MeSH data available.


Related in: MedlinePlus

The viscosity of milk fermented with 54 mutant strains and the control* (n=3, Mean±SEM).* No. 1 - No. 54 represented the 54 mutant strains and the No. 55 represented the control (initial strain).
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Figure 1: The viscosity of milk fermented with 54 mutant strains and the control* (n=3, Mean±SEM).* No. 1 - No. 54 represented the 54 mutant strains and the No. 55 represented the control (initial strain).

Mentions: 54 mutant strains were obtained by 0.2 % diethyl sulfate and the viscosity of the milk fermented by the 54 mutant strains was determined and the results were shown in Figure 1(Fig. 1). It showed that there was no significant difference between the viscosities of milk fermented by 8 mutations (No. 17, No. 18, No. 19, No. 27, No. 28, No. 31, No. 32, and No. 34) and that of initial strains (P > 0.05). Among the other mutations, the viscosity of milk fermented with 19 mutant strains rose significantly compared with that of initial strains (P < 0.05), while the other 27 mutant strains decreased the viscosity of milk significantly (P < 0.05). The highest viscosity of the fermented milk was 3303.3±17.5 mp·s.


Yield improvement of exopolysaccharides by screening of the Lactobacillus acidophilus ATCC and optimization of the fermentation and extraction conditions.

Liu Q, Huang X, Yang D, Si T, Pan S, Yang F - EXCLI J (2016)

The viscosity of milk fermented with 54 mutant strains and the control* (n=3, Mean±SEM).* No. 1 - No. 54 represented the 54 mutant strains and the No. 55 represented the control (initial strain).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834753&req=5

Figure 1: The viscosity of milk fermented with 54 mutant strains and the control* (n=3, Mean±SEM).* No. 1 - No. 54 represented the 54 mutant strains and the No. 55 represented the control (initial strain).
Mentions: 54 mutant strains were obtained by 0.2 % diethyl sulfate and the viscosity of the milk fermented by the 54 mutant strains was determined and the results were shown in Figure 1(Fig. 1). It showed that there was no significant difference between the viscosities of milk fermented by 8 mutations (No. 17, No. 18, No. 19, No. 27, No. 28, No. 31, No. 32, and No. 34) and that of initial strains (P > 0.05). Among the other mutations, the viscosity of milk fermented with 19 mutant strains rose significantly compared with that of initial strains (P < 0.05), while the other 27 mutant strains decreased the viscosity of milk significantly (P < 0.05). The highest viscosity of the fermented milk was 3303.3±17.5 mp·s.

Bottom Line: It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield.In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L.The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Engineering, Hubei University Zhixing College, Wuhan 430011, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

ABSTRACT
Exopolysacharides (EPS) produced by Lactobacillus acidophilus play an important role in food processing with its well-recognized antioxidant activity. In this study, a L. acidophilus mutant strain with high-yielding EPS (2.92±0.05 g/L) was screened by chemical mutation (0.2 % diethyl sulfate). Plackett-Burman (PB) design and response surface methodology (RSM) were applied to optimize the EPS fermentation parameters and central composite design (CCD) was used to optimize the EPS extraction parameters. A strain with high-yielding EPS was screened. It was revealed that three parameters (Tween 80, dipotassium hydrogen phosphate and trisodium citrate) had significant influence (P < 0.05) on the EPS yield. The optimal culture conditions for EPS production were: Tween 80 0.6 mL, dipotassium hydrogen phosphate 3.6 g and trisodium citrate 4.1 g (with culture volume of 1 L). In these conditions, the maximum EPS yield was 3.96±0.08 g/L. The optimal extraction conditions analyzed by CCD were: alcohol concentration 70 %, the ratio of material to liquid (M/L ratio) 1:3.6 and the extraction time 31 h. In these conditions, the maximum EPS extraction yield was 1.48±0.23 g/L. It was confirmed by the verification experiments that the EPS yield from L. acidophilus mutant strains reached 5.12±0.73 g/L under the optimized fermentation and extraction conditions, which was 3.8 times higher than that of the control (1.05±0.06 g/L). The results indicated that the strain screening with high-yielding EPS was successful and the optimized fermentation and extraction conditions significantly enhanced EPS yield. It was efficient and industrially promising.

No MeSH data available.


Related in: MedlinePlus