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Prognostic and Clinicopathological Significance of Transducer-Like Enhancer of Split 1 Expression in Gastric Cancer.

Lee JH, Son MW, Kim KJ, Oh MH, Cho H, Lee HJ, Jang SH, Lee MS - J Gastric Cancer (2016)

Bottom Line: The expression of TLE1 was significantly associated with male gender (P=0.021), less frequent lymphatic (P=0.017) or perineural invasion (P=0.029), intestinal type according to the Lauren classification (P=0.024), good histologic grade (P<0.001), early pathologic T-stage (P=0.012), and early American Joint Committee on Cancer stage (P=0.022).In the Kaplan-Meier analysis, the TLE1 expression was significantly associated with longer disease-free (P=0.022) and overall (P=0.001) survival rates.We suggested that TLE1 expression is a good prognostic indicator in GCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Soonchunhyang University Cheonan Hospital, Cheonan, Korea.

ABSTRACT

Purpose: Transducer-like enhancer of split 1 (TLE1) is a member of the Groucho/TLE family of transcriptional co-repressors that regulate the transcriptional activity of numerous genes. TLE1 is involved in the tumorigenesis of various tumors. We investigated the prognostic significance of TLE1 expression and its association with clinicopathological parameters in gastric cancer (GC) patients.

Materials and methods: Immunohistochemical analysis of six tissue microarrays was performed to examine TLE1 expression using 291 surgically resected GC specimens from the Soonchunhyang University Cheonan Hospital between July 2006 and December 2009.

Results: In the non-neoplastic gastric mucosa, TLE1 expression was negative. In GC, 121 patients (41.6%) were positive for TLE1. The expression of TLE1 was significantly associated with male gender (P=0.021), less frequent lymphatic (P=0.017) or perineural invasion (P=0.029), intestinal type according to the Lauren classification (P=0.024), good histologic grade (P<0.001), early pathologic T-stage (P=0.012), and early American Joint Committee on Cancer stage (P=0.022). In the Kaplan-Meier analysis, the TLE1 expression was significantly associated with longer disease-free (P=0.022) and overall (P=0.001) survival rates.

Conclusions: We suggested that TLE1 expression is a good prognostic indicator in GCs.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical (IHC) staining of transducer-like enhancer of split 1 (TLE1) in normal gastric mucosa and gastric cancer (GC). (A) Representative images of TLE1 IHC of normal gastric mucosa (×200) exhibiting a score of 0. (B) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 1. (C) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 2. (D) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 3.
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Figure 1: Immunohistochemical (IHC) staining of transducer-like enhancer of split 1 (TLE1) in normal gastric mucosa and gastric cancer (GC). (A) Representative images of TLE1 IHC of normal gastric mucosa (×200) exhibiting a score of 0. (B) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 1. (C) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 2. (D) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 3.

Mentions: IHC staining was independently evaluated by JH Lee and KJ Kim, and in the rare instances where there was a discrepancy in their judgment, the two investigators reviewed the slides together using a multi-head microscope. Semi-quantitative IHC scores were assigned that included an assessment of both intensity and the extent of staining. The intensity of staining was scored on a scale of 0 to 3, corresponding to negative, weak, moderate, and strong positivity, respectively (Fig. 1). The extent of staining was also scored on a scale of 0 to 3, according to the percentages of cells (0, ≤10, >10 and ≤50, or >50%, respectively) that stained positively for each protein. The product of the intensity and extent scores was used to denote the final score (i.e., 0, 1, 2, 3, 4, 6, or 9). Tissues with a final score of ≥2 were considered positive for TLE1 expression. Similar semi-quantitative scoring systems have been successfully used for other TMA assessments.19


Prognostic and Clinicopathological Significance of Transducer-Like Enhancer of Split 1 Expression in Gastric Cancer.

Lee JH, Son MW, Kim KJ, Oh MH, Cho H, Lee HJ, Jang SH, Lee MS - J Gastric Cancer (2016)

Immunohistochemical (IHC) staining of transducer-like enhancer of split 1 (TLE1) in normal gastric mucosa and gastric cancer (GC). (A) Representative images of TLE1 IHC of normal gastric mucosa (×200) exhibiting a score of 0. (B) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 1. (C) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 2. (D) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834617&req=5

Figure 1: Immunohistochemical (IHC) staining of transducer-like enhancer of split 1 (TLE1) in normal gastric mucosa and gastric cancer (GC). (A) Representative images of TLE1 IHC of normal gastric mucosa (×200) exhibiting a score of 0. (B) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 1. (C) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 2. (D) Representative images of TLE1 IHC of GCs (×400) exhibiting a score of 3.
Mentions: IHC staining was independently evaluated by JH Lee and KJ Kim, and in the rare instances where there was a discrepancy in their judgment, the two investigators reviewed the slides together using a multi-head microscope. Semi-quantitative IHC scores were assigned that included an assessment of both intensity and the extent of staining. The intensity of staining was scored on a scale of 0 to 3, corresponding to negative, weak, moderate, and strong positivity, respectively (Fig. 1). The extent of staining was also scored on a scale of 0 to 3, according to the percentages of cells (0, ≤10, >10 and ≤50, or >50%, respectively) that stained positively for each protein. The product of the intensity and extent scores was used to denote the final score (i.e., 0, 1, 2, 3, 4, 6, or 9). Tissues with a final score of ≥2 were considered positive for TLE1 expression. Similar semi-quantitative scoring systems have been successfully used for other TMA assessments.19

Bottom Line: The expression of TLE1 was significantly associated with male gender (P=0.021), less frequent lymphatic (P=0.017) or perineural invasion (P=0.029), intestinal type according to the Lauren classification (P=0.024), good histologic grade (P<0.001), early pathologic T-stage (P=0.012), and early American Joint Committee on Cancer stage (P=0.022).In the Kaplan-Meier analysis, the TLE1 expression was significantly associated with longer disease-free (P=0.022) and overall (P=0.001) survival rates.We suggested that TLE1 expression is a good prognostic indicator in GCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Soonchunhyang University Cheonan Hospital, Cheonan, Korea.

ABSTRACT

Purpose: Transducer-like enhancer of split 1 (TLE1) is a member of the Groucho/TLE family of transcriptional co-repressors that regulate the transcriptional activity of numerous genes. TLE1 is involved in the tumorigenesis of various tumors. We investigated the prognostic significance of TLE1 expression and its association with clinicopathological parameters in gastric cancer (GC) patients.

Materials and methods: Immunohistochemical analysis of six tissue microarrays was performed to examine TLE1 expression using 291 surgically resected GC specimens from the Soonchunhyang University Cheonan Hospital between July 2006 and December 2009.

Results: In the non-neoplastic gastric mucosa, TLE1 expression was negative. In GC, 121 patients (41.6%) were positive for TLE1. The expression of TLE1 was significantly associated with male gender (P=0.021), less frequent lymphatic (P=0.017) or perineural invasion (P=0.029), intestinal type according to the Lauren classification (P=0.024), good histologic grade (P<0.001), early pathologic T-stage (P=0.012), and early American Joint Committee on Cancer stage (P=0.022). In the Kaplan-Meier analysis, the TLE1 expression was significantly associated with longer disease-free (P=0.022) and overall (P=0.001) survival rates.

Conclusions: We suggested that TLE1 expression is a good prognostic indicator in GCs.

No MeSH data available.


Related in: MedlinePlus