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Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.

Li S, Yue W, Wang M, Qiu W, Zhou L, Shou H - Front Plant Sci (2016)

Bottom Line: In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice.Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement.In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; College of Life Sciences, Qingdao Agricultural UniversityQingdao, China.

ABSTRACT
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

No MeSH data available.


Related in: MedlinePlus

Analysis of stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants. Stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants after PEG treatment for 1, 3, and 5 h. 35-day-old WT and osgi plants grown under normal conditions or treated with 18% PEG 8000 at 4, 6, and 8 h after lights-on, respectively, were used for the experiment. The gs and Tr were recorded in osgi and WT plants using an Li-6400 at 9 h after lights-on. All data represent the mean of three biological replicates, with error bars indicating SD.
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Figure 6: Analysis of stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants. Stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants after PEG treatment for 1, 3, and 5 h. 35-day-old WT and osgi plants grown under normal conditions or treated with 18% PEG 8000 at 4, 6, and 8 h after lights-on, respectively, were used for the experiment. The gs and Tr were recorded in osgi and WT plants using an Li-6400 at 9 h after lights-on. All data represent the mean of three biological replicates, with error bars indicating SD.

Mentions: Under normal growth conditions, there was no difference in the gs and Trbetween WT and osgi plants (Figure 6). PEG treatment reduced the gs and Tr in both WT and osgi plants. The gs decreased by 70, 75, and 90% in WT plants after PEG treatment for 1, 3, and 5 h, respectively. In contrast, the gs in osgi seedlings decreased rapidly by about 90% after only 1 h of PEG treatment and stayed at a similarly low level after 3 and 5 h of PEG exposure. The change in Tr in response to PEG treatment followed a similar pattern as gs (Figure 6). These results indicate that under osmotic stress conditions, stomata movement changes faster in osgi mutant plants than in WT plants.


Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.

Li S, Yue W, Wang M, Qiu W, Zhou L, Shou H - Front Plant Sci (2016)

Analysis of stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants. Stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants after PEG treatment for 1, 3, and 5 h. 35-day-old WT and osgi plants grown under normal conditions or treated with 18% PEG 8000 at 4, 6, and 8 h after lights-on, respectively, were used for the experiment. The gs and Tr were recorded in osgi and WT plants using an Li-6400 at 9 h after lights-on. All data represent the mean of three biological replicates, with error bars indicating SD.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
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Figure 6: Analysis of stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants. Stomata conductance (gs) and transpiration rate (Tr) in WT and osgi plants after PEG treatment for 1, 3, and 5 h. 35-day-old WT and osgi plants grown under normal conditions or treated with 18% PEG 8000 at 4, 6, and 8 h after lights-on, respectively, were used for the experiment. The gs and Tr were recorded in osgi and WT plants using an Li-6400 at 9 h after lights-on. All data represent the mean of three biological replicates, with error bars indicating SD.
Mentions: Under normal growth conditions, there was no difference in the gs and Trbetween WT and osgi plants (Figure 6). PEG treatment reduced the gs and Tr in both WT and osgi plants. The gs decreased by 70, 75, and 90% in WT plants after PEG treatment for 1, 3, and 5 h, respectively. In contrast, the gs in osgi seedlings decreased rapidly by about 90% after only 1 h of PEG treatment and stayed at a similarly low level after 3 and 5 h of PEG exposure. The change in Tr in response to PEG treatment followed a similar pattern as gs (Figure 6). These results indicate that under osmotic stress conditions, stomata movement changes faster in osgi mutant plants than in WT plants.

Bottom Line: In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice.Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement.In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; College of Life Sciences, Qingdao Agricultural UniversityQingdao, China.

ABSTRACT
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

No MeSH data available.


Related in: MedlinePlus