Limits...
Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.

Li S, Yue W, Wang M, Qiu W, Zhou L, Shou H - Front Plant Sci (2016)

Bottom Line: In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice.Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement.In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; College of Life Sciences, Qingdao Agricultural UniversityQingdao, China.

ABSTRACT
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

No MeSH data available.


Related in: MedlinePlus

Analysis of proline and sucrose contents in WT and osgi plants. Proline and sucrose contents in leaves of 15-day-old WT and osgi plants grown under normal conditions. All data represent the mean of three biological replicates, with error bars indicating SD. The unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4834575&req=5

Figure 5: Analysis of proline and sucrose contents in WT and osgi plants. Proline and sucrose contents in leaves of 15-day-old WT and osgi plants grown under normal conditions. All data represent the mean of three biological replicates, with error bars indicating SD. The unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively.

Mentions: It is generally accepted that under abiotic stress conditions, plants often accumulate compatible osmolytes to maintain osmotic balance and protect their subcellular structures from damage. Several studies have shown that the accumulation of free proline is positively correlated to plant tolerance to dehydration stress (Shinozaki and Yamaguchi-Shinozaki, 2007; Xiong et al., 2012). In order to assess whether mutation of OsGI can enhance the accumulation of osmotic protectants, the free proline content in WT and osgi plants was analyzed (Figure 5). Leaves from plants grown under normal conditions were collected at four different time points at 15 DAG and the proline content measured. At all times, the proline content was higher in osgi than in WT plants. For example, 8 h after lights-on, the proline levels in osgi plants were approximately 1.7 times higher than in WT plants (Figure 5). Moreover, while sucrose levels increased throughout the day and decreased at night in both WT and osgi plants, osgi plants had higher sucrose levels than WT plants at all times (Figure 5). The sucrose content in L1 and L2 plants was not altered compared to WT plants (Supplementary Figure 3). These results suggest that osgi plants possess a much higher osmotic potential, which could be beneficial in protecting plants against osmotic stress.


Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.

Li S, Yue W, Wang M, Qiu W, Zhou L, Shou H - Front Plant Sci (2016)

Analysis of proline and sucrose contents in WT and osgi plants. Proline and sucrose contents in leaves of 15-day-old WT and osgi plants grown under normal conditions. All data represent the mean of three biological replicates, with error bars indicating SD. The unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834575&req=5

Figure 5: Analysis of proline and sucrose contents in WT and osgi plants. Proline and sucrose contents in leaves of 15-day-old WT and osgi plants grown under normal conditions. All data represent the mean of three biological replicates, with error bars indicating SD. The unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively.
Mentions: It is generally accepted that under abiotic stress conditions, plants often accumulate compatible osmolytes to maintain osmotic balance and protect their subcellular structures from damage. Several studies have shown that the accumulation of free proline is positively correlated to plant tolerance to dehydration stress (Shinozaki and Yamaguchi-Shinozaki, 2007; Xiong et al., 2012). In order to assess whether mutation of OsGI can enhance the accumulation of osmotic protectants, the free proline content in WT and osgi plants was analyzed (Figure 5). Leaves from plants grown under normal conditions were collected at four different time points at 15 DAG and the proline content measured. At all times, the proline content was higher in osgi than in WT plants. For example, 8 h after lights-on, the proline levels in osgi plants were approximately 1.7 times higher than in WT plants (Figure 5). Moreover, while sucrose levels increased throughout the day and decreased at night in both WT and osgi plants, osgi plants had higher sucrose levels than WT plants at all times (Figure 5). The sucrose content in L1 and L2 plants was not altered compared to WT plants (Supplementary Figure 3). These results suggest that osgi plants possess a much higher osmotic potential, which could be beneficial in protecting plants against osmotic stress.

Bottom Line: In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice.Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement.In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; College of Life Sciences, Qingdao Agricultural UniversityQingdao, China.

ABSTRACT
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

No MeSH data available.


Related in: MedlinePlus