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Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.

Li S, Yue W, Wang M, Qiu W, Zhou L, Shou H - Front Plant Sci (2016)

Bottom Line: In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice.Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement.In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; College of Life Sciences, Qingdao Agricultural UniversityQingdao, China.

ABSTRACT
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

No MeSH data available.


Related in: MedlinePlus

Characterization of the growth phenotype of WT and osgi plants. (A) Expression of OsGI in WT and osgi (gi) plants throughout the day. Unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively. Gene expression was normalized to the expression level of OsACTIN. (B) WT and osgi plants were grown hydroponically under normal growth conditions for 30 days. (C) Shoot length, root length and root/shoot ratio of WT and osgi plants. All data represent the mean of three biological replicates, with error bars indicating SD. Significant differences relative to the corresponding WT strain are indicated with asterisks (***P < 0.001; and **P < 0.01). Bars = 4 cm. nd, not detectable.
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Figure 1: Characterization of the growth phenotype of WT and osgi plants. (A) Expression of OsGI in WT and osgi (gi) plants throughout the day. Unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively. Gene expression was normalized to the expression level of OsACTIN. (B) WT and osgi plants were grown hydroponically under normal growth conditions for 30 days. (C) Shoot length, root length and root/shoot ratio of WT and osgi plants. All data represent the mean of three biological replicates, with error bars indicating SD. Significant differences relative to the corresponding WT strain are indicated with asterisks (***P < 0.001; and **P < 0.01). Bars = 4 cm. nd, not detectable.

Mentions: The TOS17 insertion mutant of osgi was obtained and characterized. As shown in Figure 1, the cyclic expression pattern of OsGI, which peaks at dusk in wild type (WT) plants, was completely abolished in osgi mutant plants (Figure 1A). Circadian rhythm genes, such as OsRFT1 and OsHd3a, were also suppressed in osgi plants (Supplementary Figure 1). Osgi mutants displayed a growth inhibited phenotype (Figure 1B; Itoh and Izawa, 2011). Shoot and root lengths of 30-day-old osgi plants were reduced by 35 and 15% compared to WT plants, respectively, resulting in an increased root-to-shoot ratio (Figure 1C).


Mutation of OsGIGANTEA Leads to Enhanced Tolerance to Polyethylene Glycol-Generated Osmotic Stress in Rice.

Li S, Yue W, Wang M, Qiu W, Zhou L, Shou H - Front Plant Sci (2016)

Characterization of the growth phenotype of WT and osgi plants. (A) Expression of OsGI in WT and osgi (gi) plants throughout the day. Unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively. Gene expression was normalized to the expression level of OsACTIN. (B) WT and osgi plants were grown hydroponically under normal growth conditions for 30 days. (C) Shoot length, root length and root/shoot ratio of WT and osgi plants. All data represent the mean of three biological replicates, with error bars indicating SD. Significant differences relative to the corresponding WT strain are indicated with asterisks (***P < 0.001; and **P < 0.01). Bars = 4 cm. nd, not detectable.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834575&req=5

Figure 1: Characterization of the growth phenotype of WT and osgi plants. (A) Expression of OsGI in WT and osgi (gi) plants throughout the day. Unfilled and filled bars below the x-axis indicate times of lights-on and lights-off, respectively. Gene expression was normalized to the expression level of OsACTIN. (B) WT and osgi plants were grown hydroponically under normal growth conditions for 30 days. (C) Shoot length, root length and root/shoot ratio of WT and osgi plants. All data represent the mean of three biological replicates, with error bars indicating SD. Significant differences relative to the corresponding WT strain are indicated with asterisks (***P < 0.001; and **P < 0.01). Bars = 4 cm. nd, not detectable.
Mentions: The TOS17 insertion mutant of osgi was obtained and characterized. As shown in Figure 1, the cyclic expression pattern of OsGI, which peaks at dusk in wild type (WT) plants, was completely abolished in osgi mutant plants (Figure 1A). Circadian rhythm genes, such as OsRFT1 and OsHd3a, were also suppressed in osgi plants (Supplementary Figure 1). Osgi mutants displayed a growth inhibited phenotype (Figure 1B; Itoh and Izawa, 2011). Shoot and root lengths of 30-day-old osgi plants were reduced by 35 and 15% compared to WT plants, respectively, resulting in an increased root-to-shoot ratio (Figure 1C).

Bottom Line: In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice.Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement.In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang UniversityHangzhou, China; College of Life Sciences, Qingdao Agricultural UniversityQingdao, China.

ABSTRACT
Water deficit is one of the most important environmental stresses limiting plant growth and crop yield. While the identification of many key factors involved in the plant water deficit response has greatly increased our knowledge about the regulation system, the mechanisms underlying dehydration tolerance in plants are still not well understood. In our current study, we investigated the roles of the key flowering time regulator, OsGIGANTEA (OsGI), in the osmotic stress tolerance in rice. Results showed that mutation of OsGI conferred tolerance to osmotic stress generated by polyethylene glycol (PEG), increased proline and sucrose contents, and accelerated stomata movement. In addition, qRT-PCR and microarray analysis revealed that the transcript abundance of some osmotic stress response genes, such as OsDREB1E, OsAP37, OsAP59, OsLIP9, OsLEA3, OsRAB16A, and OsSalT, was significantly higher in osgi than in WT plants, suggesting that OsGI might be a negative regulator in the osmotic stress response in rice.

No MeSH data available.


Related in: MedlinePlus