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The reproductive tracts of two malaria vectors are populated by a core microbiome and by gender- and swarm-enriched microbial biomarkers.

Segata N, Baldini F, Pompon J, Garrett WS, Truong DT, Dabiré RK, Diabaté A, Levashina EA, Catteruccia F - Sci Rep (2016)

Bottom Line: Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens.We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues.Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site.

View Article: PubMed Central - PubMed

Affiliation: Centre for Integrative Biology, University of Trento, Trento, Italy.

ABSTRACT
Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens. In Sub-Saharan Africa, two major malaria vectors, Anopheles gambiae and An. coluzzii, breed in distinct larval habitats characterized by different microorganisms that might affect their adult physiology and possibly Plasmodium transmission. We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues. Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site. Finally, we identified several endosymbiotic bacteria, including Spiroplasma, which have the ability to manipulate insect reproductive success. Our study provides a comprehensive analysis of the reproductive microbiome of important human disease vectors, and identifies a panel of core and endosymbiotic bacteria that can be potentially exploited to interfere with the transmission of malaria parasites by the Anopheles mosquito.

No MeSH data available.


Related in: MedlinePlus

The most variable taxa in the An. gambiae and An. coluzzii reproductive tract microbiomes.The 30 OTUs with highest score are reported here after computing the coefficient of variation for all the OTUs in our dataset. Each value in the heatmap represents the relative abundance of an OTU in a sample.
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f5: The most variable taxa in the An. gambiae and An. coluzzii reproductive tract microbiomes.The 30 OTUs with highest score are reported here after computing the coefficient of variation for all the OTUs in our dataset. Each value in the heatmap represents the relative abundance of an OTU in a sample.

Mentions: Even in the presence of a large core microbiome, we observed high alpha diversity (i.e. intra-sample diversity) due to the presence of many bacteria with partial prevalence but occasional high abundance. Many OTUs showed a large coefficient of variation across samples (Fig. 5), including members of Enterobacteriaceae, Pseudomonadaceae, Achromobacter, and Deftia. These highly variable OTUs were not statistically correlated with any of the metadata available (gender, species, tissue, village), which may suggest they are not strictly required for normal micro-ecology and host-microbiome homeostasis, and may result from local environmental acquisition events, specific amplification in favorable conditions, or horizontal transmission.


The reproductive tracts of two malaria vectors are populated by a core microbiome and by gender- and swarm-enriched microbial biomarkers.

Segata N, Baldini F, Pompon J, Garrett WS, Truong DT, Dabiré RK, Diabaté A, Levashina EA, Catteruccia F - Sci Rep (2016)

The most variable taxa in the An. gambiae and An. coluzzii reproductive tract microbiomes.The 30 OTUs with highest score are reported here after computing the coefficient of variation for all the OTUs in our dataset. Each value in the heatmap represents the relative abundance of an OTU in a sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834568&req=5

f5: The most variable taxa in the An. gambiae and An. coluzzii reproductive tract microbiomes.The 30 OTUs with highest score are reported here after computing the coefficient of variation for all the OTUs in our dataset. Each value in the heatmap represents the relative abundance of an OTU in a sample.
Mentions: Even in the presence of a large core microbiome, we observed high alpha diversity (i.e. intra-sample diversity) due to the presence of many bacteria with partial prevalence but occasional high abundance. Many OTUs showed a large coefficient of variation across samples (Fig. 5), including members of Enterobacteriaceae, Pseudomonadaceae, Achromobacter, and Deftia. These highly variable OTUs were not statistically correlated with any of the metadata available (gender, species, tissue, village), which may suggest they are not strictly required for normal micro-ecology and host-microbiome homeostasis, and may result from local environmental acquisition events, specific amplification in favorable conditions, or horizontal transmission.

Bottom Line: Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens.We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues.Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site.

View Article: PubMed Central - PubMed

Affiliation: Centre for Integrative Biology, University of Trento, Trento, Italy.

ABSTRACT
Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens. In Sub-Saharan Africa, two major malaria vectors, Anopheles gambiae and An. coluzzii, breed in distinct larval habitats characterized by different microorganisms that might affect their adult physiology and possibly Plasmodium transmission. We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues. Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site. Finally, we identified several endosymbiotic bacteria, including Spiroplasma, which have the ability to manipulate insect reproductive success. Our study provides a comprehensive analysis of the reproductive microbiome of important human disease vectors, and identifies a panel of core and endosymbiotic bacteria that can be potentially exploited to interfere with the transmission of malaria parasites by the Anopheles mosquito.

No MeSH data available.


Related in: MedlinePlus