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The reproductive tracts of two malaria vectors are populated by a core microbiome and by gender- and swarm-enriched microbial biomarkers.

Segata N, Baldini F, Pompon J, Garrett WS, Truong DT, Dabiré RK, Diabaté A, Levashina EA, Catteruccia F - Sci Rep (2016)

Bottom Line: Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens.We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues.Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site.

View Article: PubMed Central - PubMed

Affiliation: Centre for Integrative Biology, University of Trento, Trento, Italy.

ABSTRACT
Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens. In Sub-Saharan Africa, two major malaria vectors, Anopheles gambiae and An. coluzzii, breed in distinct larval habitats characterized by different microorganisms that might affect their adult physiology and possibly Plasmodium transmission. We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues. Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site. Finally, we identified several endosymbiotic bacteria, including Spiroplasma, which have the ability to manipulate insect reproductive success. Our study provides a comprehensive analysis of the reproductive microbiome of important human disease vectors, and identifies a panel of core and endosymbiotic bacteria that can be potentially exploited to interfere with the transmission of malaria parasites by the Anopheles mosquito.

No MeSH data available.


Related in: MedlinePlus

Schematic description of villages and mosquito swarm sites.The maps describe the three villages (Soumousso, VK5 and VK7) where An. gambiae and An. coluzzii couples were collected from mating swarms. Swarm sites are identified by numbers. Maps were modified from Fig. 1 published in20, originally adapted from A.A. Millogo, IRSS/Muraz.
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f1: Schematic description of villages and mosquito swarm sites.The maps describe the three villages (Soumousso, VK5 and VK7) where An. gambiae and An. coluzzii couples were collected from mating swarms. Swarm sites are identified by numbers. Maps were modified from Fig. 1 published in20, originally adapted from A.A. Millogo, IRSS/Muraz.

Mentions: To characterize the reproductive tract microbiomes of An. gambiae and An. coluzzii adults, mating couples were collected in natural swarms in three villages near Bobo-Dioulasso, Burkina Faso: Vallée du Kou 5 (VK5) and Vallée du Kou 7 (VK7), highly populated by An. coluzzii, and Soumousso, where An. gambiae are predominant (see Fig. 1 and Supplementary Table S1). Four reproductive tissues were isolated: the testes and male accessory glands (MAGs) from males, and the ovaries and lower reproductive tract (LRT, which comprises the atrium, the spermatheca and the parovarium) from females. We collected mating couples rather than resting males and females as we wanted to specifically study sexually active individuals. While the vast majority of collections (26 out of 30 mating couples) were composed of conspecific couples, we found 4 mixed An. gambiae/An. coluzzii couples, providing a 13.3% frequency of interspecific matings similar to previously reported hybridization frequencies in this geographical area25.


The reproductive tracts of two malaria vectors are populated by a core microbiome and by gender- and swarm-enriched microbial biomarkers.

Segata N, Baldini F, Pompon J, Garrett WS, Truong DT, Dabiré RK, Diabaté A, Levashina EA, Catteruccia F - Sci Rep (2016)

Schematic description of villages and mosquito swarm sites.The maps describe the three villages (Soumousso, VK5 and VK7) where An. gambiae and An. coluzzii couples were collected from mating swarms. Swarm sites are identified by numbers. Maps were modified from Fig. 1 published in20, originally adapted from A.A. Millogo, IRSS/Muraz.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834568&req=5

f1: Schematic description of villages and mosquito swarm sites.The maps describe the three villages (Soumousso, VK5 and VK7) where An. gambiae and An. coluzzii couples were collected from mating swarms. Swarm sites are identified by numbers. Maps were modified from Fig. 1 published in20, originally adapted from A.A. Millogo, IRSS/Muraz.
Mentions: To characterize the reproductive tract microbiomes of An. gambiae and An. coluzzii adults, mating couples were collected in natural swarms in three villages near Bobo-Dioulasso, Burkina Faso: Vallée du Kou 5 (VK5) and Vallée du Kou 7 (VK7), highly populated by An. coluzzii, and Soumousso, where An. gambiae are predominant (see Fig. 1 and Supplementary Table S1). Four reproductive tissues were isolated: the testes and male accessory glands (MAGs) from males, and the ovaries and lower reproductive tract (LRT, which comprises the atrium, the spermatheca and the parovarium) from females. We collected mating couples rather than resting males and females as we wanted to specifically study sexually active individuals. While the vast majority of collections (26 out of 30 mating couples) were composed of conspecific couples, we found 4 mixed An. gambiae/An. coluzzii couples, providing a 13.3% frequency of interspecific matings similar to previously reported hybridization frequencies in this geographical area25.

Bottom Line: Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens.We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues.Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site.

View Article: PubMed Central - PubMed

Affiliation: Centre for Integrative Biology, University of Trento, Trento, Italy.

ABSTRACT
Microbes play key roles in shaping the physiology of insects and can influence behavior, reproduction and susceptibility to pathogens. In Sub-Saharan Africa, two major malaria vectors, Anopheles gambiae and An. coluzzii, breed in distinct larval habitats characterized by different microorganisms that might affect their adult physiology and possibly Plasmodium transmission. We analyzed the reproductive microbiomes of male and female An. gambiae and An. coluzzii couples collected from natural mating swarms in Burkina Faso. 16S rRNA sequencing on dissected tissues revealed that the reproductive tracts harbor a complex microbiome characterized by a large core group of bacteria shared by both species and all reproductive tissues. Interestingly, we detected a significant enrichment of several gender-associated microbial biomarkers in specific tissues, and surprisingly, similar classes of bacteria in males captured from one mating swarm, suggesting that these males originated from the same larval breeding site. Finally, we identified several endosymbiotic bacteria, including Spiroplasma, which have the ability to manipulate insect reproductive success. Our study provides a comprehensive analysis of the reproductive microbiome of important human disease vectors, and identifies a panel of core and endosymbiotic bacteria that can be potentially exploited to interfere with the transmission of malaria parasites by the Anopheles mosquito.

No MeSH data available.


Related in: MedlinePlus