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Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus

Migration of keratinocytes in the wound.(a) The expression of E-cadherin at the wound edge at 3 days post-wounding, which revealed that the staining of E-cadherin at the wound edge decreased without a typical linear pattern when MP-PU membrane was applied. (b) E-cadherin and β-actin protein levels were determined by Western blot, and (c) relative densities of E-cadherin protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01.
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f9: Migration of keratinocytes in the wound.(a) The expression of E-cadherin at the wound edge at 3 days post-wounding, which revealed that the staining of E-cadherin at the wound edge decreased without a typical linear pattern when MP-PU membrane was applied. (b) E-cadherin and β-actin protein levels were determined by Western blot, and (c) relative densities of E-cadherin protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01.

Mentions: Second, we investigated the migration of keratinocyte in the neo-epithelium at the wound edge. As one of the components of adherens junctions, downregulation of E-cadherin might lead to a loosening of cell-cell contact, which contributes to keratinocyte migration15. The expression of E-cadherin was detected by immunofluorescence and we observed that the staining of E-cadherin in blank and EHP groups exhibited a typical linear pattern outlining the cell. However, the staining of E-cadherin in other groups was weak and blurred especially in the HP and MP groups (Fig. 9a), which indicated that expression of E-cadherin was down regulated and thus led to the keratinocyte migration at the wound edge. Western blot analysis also revealed that expression of the E-cadherin in the blank group was significantly higher than that in the other groups (Fig. 9b,c, the relative density of E-cadherin in blank group was larger than that of all the others).


Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement.

Xu R, Xia H, He W, Li Z, Zhao J, Liu B, Wang Y, Lei Q, Kong Y, Bai Y, Yao Z, Yan R, Li H, Zhan R, Yang S, Luo G, Wu J - Sci Rep (2016)

Migration of keratinocytes in the wound.(a) The expression of E-cadherin at the wound edge at 3 days post-wounding, which revealed that the staining of E-cadherin at the wound edge decreased without a typical linear pattern when MP-PU membrane was applied. (b) E-cadherin and β-actin protein levels were determined by Western blot, and (c) relative densities of E-cadherin protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4834567&req=5

f9: Migration of keratinocytes in the wound.(a) The expression of E-cadherin at the wound edge at 3 days post-wounding, which revealed that the staining of E-cadherin at the wound edge decreased without a typical linear pattern when MP-PU membrane was applied. (b) E-cadherin and β-actin protein levels were determined by Western blot, and (c) relative densities of E-cadherin protein level in each group are shown. The values were calculated as the mean ± SD (n = 3), **p < 0.01.
Mentions: Second, we investigated the migration of keratinocyte in the neo-epithelium at the wound edge. As one of the components of adherens junctions, downregulation of E-cadherin might lead to a loosening of cell-cell contact, which contributes to keratinocyte migration15. The expression of E-cadherin was detected by immunofluorescence and we observed that the staining of E-cadherin in blank and EHP groups exhibited a typical linear pattern outlining the cell. However, the staining of E-cadherin in other groups was weak and blurred especially in the HP and MP groups (Fig. 9a), which indicated that expression of E-cadherin was down regulated and thus led to the keratinocyte migration at the wound edge. Western blot analysis also revealed that expression of the E-cadherin in the blank group was significantly higher than that in the other groups (Fig. 9b,c, the relative density of E-cadherin in blank group was larger than that of all the others).

Bottom Line: Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies.It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model.Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model.

View Article: PubMed Central - PubMed

Affiliation: Institute of Burn Research, Southwest Hospital; State Key Lab of Trauma, Burn and Combined Injury; Chongqing Key Laboratory for Disease Proteomics, Third Military Medical University, Chongqing 400038, China.

ABSTRACT
A desirable microenvironment is essential for wound healing, in which an ideal moisture content is one of the most important factors. The fundamental function and requirement for wound dressings is to keep the wound at an optimal moisture. Here, we prepared serial polyurethane (PU) membrane dressings with graded water vapor transmission rates (WVTRs), and the optimal WVTR of the dressing for wound healing was identified by both in vitro and in vivo studies. It was found that the dressing with a WVTR of 2028.3 ± 237.8 g/m(2)·24 h was able to maintain an optimal moisture content for the proliferation and regular function of epidermal cells and fibroblasts in a three-dimensional culture model. Moreover, the dressing with this optimal WTVR was found to be able to promote wound healing in a mouse skin wound model. Our finds may be helpful in the design of wound dressing for wound regeneration in the future.

No MeSH data available.


Related in: MedlinePlus